The determinant factors underlying habitat selectivity and preference for Black fish Capoeta capoeta gracilis (Keyserling 1891) in Siyahrud River (a tributary of Sefidrud River basin)
H.
Asadi
author
M.
Sattari
author
S.
Eagderi
author
text
article
2014
per
Understanding habitat requirements of aquatic organisms is key factor for sustainable exploitation and biodiversity conservation of riverine ecosystems. The habitat preference in fish has extremely important for the management of fish populations. The habitat requirements of Black fish (Capoeta capoeta gracilis) in Siyahrud River -tributaries of the Sefidrud River in Caspian basin- were studied in 13 stations along the river during October to November 2013. Hydrological characteristics of the river including water velocity, depth and width, slope, altitude, type of substrate materials, and plant coverage were recorded for each station. Ranges of habitat use and habitat selectivity with regard to the availability of habitat units for different variables were measured using HABSEL software. Despite significant correlation between the water velocity and depth, results from multiple regressions showed preference for the depth range of 17 to 38 cm. The results showed that behavioral preference in C. capoeta gracilis for the velocity was between 0.44 and 0.56 m/s. Also, specimens tended to be present in cobble stone substrate. Therefore, the anthropogenic activities over the Siyahrod River changing the river width, depth, substrate and the flow rate may affect the presence and distribution of C. capoeta gracilis in the mentioned river.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
1
10
https://isfj.areeo.ac.ir/article_103536_132e48ab093ddfb72be170bdb766db08.pdf
dx.doi.org/10.22092/isfj.2014.103536
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota)
S.
Bahroodi
author
M. A.
Nematollahi
author
M. R.
Aghasadeghi
author
M.
Nazemi
author
B.
Behroz
author
text
article
2014
per
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
11
20
https://isfj.areeo.ac.ir/article_103537_e5ffa7ef660afe73b9239e011cf92551.pdf
dx.doi.org/10.22092/isfj.2014.103537
Effect of herbicide Atrezine chronic toxicity on bioaccumulation process in fillet of Barbus grypus
A.
Khabazian Zadeh
author
A.
Dadolahi Sohrab
author
M.
Alishahi
author
S. H.
Khazaei
author
H. Mohammad
Asgari
author
text
article
2014
per
Atrazine is one of the most important and effective pollutant in aquatic ecosystems. The largest sugar cane farms of Middle East is located in Khouzestan Province, Iran in which large amounts of Atrezine are being used in farming. The aim of this study was to investigate acute toxicity (LC50 96 h) of atrazine on barbus grypus and the effects of chronic toxicity with sub-lethal concentration of atrazine on bioaccumulation of atrazine in fish fillet. LC50 96 h of atrazine on barbus grypus was measured according to the OECD standard method, 180 barbus grypus were divided into 4 equal groups (in triplicates). Groups 1, 2 and 3 were exposed to 3.25 mg l-1 (5%), 6.5 mg l-1 (10%) and 13 mg l-1 (20%) of LC50 96 h concentrations, respectively. Group 4 exposed to toxin free water as a control group. Experimental exposures did last for 21 days, muscles samples of the large dorsal muscle were taken on days 0, 7, 14 and 21 of experiment. Bioaccumulation of Atrazine was measured in the muscle at days 0, 7, 14 and 21 in all groups. Results indicated that atrazine was toxic for barbus grypus and its toxicity increased not only with increase in atrazine but also with increase in the exposure time. The bioaccumulation of atrazine in fish muscles was increased significantly in groups 2 and 3 in all sampling periods and in groups 1 only in day 21.
The results of present study showed that placing barbus grypus in the presence of chronic and sub lethal concentrations of the herbicide Atrazine for three weeks may lead to accumulation of toxins in the fish fillets.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
21
30
https://isfj.areeo.ac.ir/article_103538_220f5bf59d94880454f886ecef00febf.pdf
dx.doi.org/10.22092/isfj.2014.103538
Effect of herbicide Atrezine chronic toxicity on bioaccumulation process in fillet of Barbus grypus
A.
Dashtiannasab
author
M.
Mesbah
author
R.
Peyghan
author
S.
Kakoolaki
author
text
article
2014
per
Atrazine is one of the most important and effective pollutant in aquatic ecosystems. The largest sugar cane farms of Middle East is located in Khouzestan Province, Iran in which large amounts of Atrezine are being used in farming. The aim of this study was to investigate acute toxicity (LC50 96 h) of atrazine on barbus grypus and the effects of chronic toxicity with sub-lethal concentration of atrazine on bioaccumulation of atrazine in fish fillet. LC50 96 h of atrazine on barbus grypus was measured according to the OECD standard method, 180 barbus grypus were divided into 4 equal groups (in triplicates). Groups 1, 2 and 3 were exposed to 3.25 mg l-1 (5%), 6.5 mg l-1 (10%) and 13 mg l-1 (20%) of LC50 96 h concentrations, respectively. Group 4 exposed to toxin free water as a control group. Experimental exposures did last for 21 days, muscles samples of the large dorsal muscle were taken on days 0, 7, 14 and 21 of experiment. Bioaccumulation of Atrazine was measured in the muscle at days 0, 7, 14 and 21 in all groups. Results indicated that atrazine was toxic for barbus grypus and its toxicity increased not only with increase in atrazine but also with increase in the exposure time. The bioaccumulation of atrazine in fish muscles was increased significantly in groups 2 and 3 in all sampling periods and in groups 1 only in day 21.
The results of present study showed that placing barbus grypus in the presence of chronic and sub lethal concentrations of the herbicide Atrazine for three weeks may lead to accumulation of toxins in the fish fillets.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
31
40
https://isfj.areeo.ac.ir/article_103539_ce3ccddb1752ad82491a3cebc4317c04.pdf
dx.doi.org/10.22092/isfj.2014.103539
Effect of nicin z on some of spoilage chemical and bacterial properties in surimi of kilka (Clupeonella cultriventris caspia) stored in 4° c
A.
Dehbandi
author
A. A.
Motallebi
author
V.
Razavilar
author
R.
Pourgholam
author
text
article
2014
per
The effect of antibacterial and antioxidant of nicin z in two forms (free and encapsulated) was investigated on Total Viable Count(TVC) ,Psychrotrophic Counts(PTC) , Peroxide Value(PV) , Thiobarbituric acid (TBA) and Total volatile nitrogen(TVN) in zero, 3, 6, 9, 12, 15 days of storage.Two concentrations (700IU/gr ,1000IU/gr) of free and encapsulated nicin in liposome (by spray-dried method) were added as spray on surimi of kilka and one treatment was selected as a control. The results showed that change of chemical and bacterial parameters in treatment of encapsulated nicin was lower than free nicin and control treatments. Concentration of 1000IU/gr of nicin was better than results of other treatments. The shelf life of surimi of kilka in control, free and encapsulated nicin treatments for bacterial results were 9,12 , 15 days, respectively, and forTBA , TVN and PV were 6 , 15 and at least 15 days, respectively. The conclusion was that encapsulated nicin in liposome improved shelf life of surimi of kilka.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
41
56
https://isfj.areeo.ac.ir/article_103540_fe604426b6802678a02bd61b45db793d.pdf
dx.doi.org/10.22092/isfj.2014.103540
Introduce of metallic pollutant reducing bacteria (Fi , Ni) in
M.
Ramin
author
M.
Doustdar
author
text
article
2014
per
In order to isolate bacterial strains resistant to iron and nickel, deposit samples were collected from five stations in Musa Bay and cultured on BHI agar medium containing 1000 ppm of iron and 50 ppm of nickel. Endurance threshold of strains isolated to different concentrations of iron (1000- 15000 ppm) and nickel (100- 25000 ppm) in BHI broth medium were evaluated, the most resistant strains were selected and MIC and MBC were determined. Results of the current study demonstrated that among 16 and 12 resistant strains to nickel and iron, two selected strains (Bacillus subtilis and Micrococcus sp. ) as the most resistant strains had MIC and MBC (29700 and 29800 ppm) and (26300 and 26500 ppm ), respectively, Ironâs MIC was not assessed. Results of atomic absorption analysis demonstrated that the highest amount of nickel reduction in a medium with concentration of 194 ppm and 71 ppm were 16.7% and 63% after 72 hours by selected strain of Bacillus subtilis respectively, and in a medium containing 158 ppm iron, after 50 hours 8.4% by selected strain 7, while dead biomass of strain 16 reduced nickel to the amount of 40.6% and it reduced 21% and 4% of iron in solutions with 46 ppm and 792 ppm of iron. Results of molecular analysis demonstrated that strain 16 was Bacillus subtilis. In addition, Bacillus subtilis being isolated in this study with ability of nickel and iron removal is an appropriate candidate to be used in aquatic environments.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
57
68
https://isfj.areeo.ac.ir/article_110172_b551d225cc67abbb89e29da1b8e37240.pdf
dx.doi.org/10.22092/isfj.2017.110172
Study of measuring Tilapia (Oreochromis niloticus) freshness using quality index method (QIM)
G.
Zarh gashti
author
A.
Motalebi
author
Y.
Moradi
author
A.
Khanipour
author
N.
Mashaii
author
H.
Jalili
author
M.
Seifzade
author
F.
Rafipour
author
F.
Lagzaii
author
text
article
2014
per
Fish freshness by using QIM and according to scoring to all of parts variable (positive and negative) was by sensory method and finally become the parameters of quantitative and qualitative with statistical analysis is based, tables used in volatility score of 0 to 23, with a score of 0 to 3 respectively, for their excellent quality, 3 to 7good, from 7 to 10 and from 10 to above average evaluation is unacceptable.
In this study to measure the freshness of fish species Nile Tilapia (Oreochromis niloticus), black and red in 4 treatments, whole fish and empty stomach is considered and the objective of this study was to calculate the survival rate of fish in ice cover practically immediately after harvest to measure qualitative factors (sensory evaluation, chemical and microbiological).For this study 50 pieces of red and 50 pieces black Tilapia after weighed, washed and then selection 25 pieces for each treatment in unlit, ratio of 3 to 1 (ice - fish) was covered .The mean (± SD) change in temperature the fish flesh during study were between 0.2 ±0.07 a to 0.3 ±0.1a degrees Celsius, that need to raise the quality temperature is maintained at 0 degrees Celsius in unlit center.
Fish covered with ice keep at ambient temperature, and after measuring the change free nitrogen, pH and microbial tests, the results showed maximum shelf life of whole fish in ice cover 9 and for empty stomach fish was7 days .And also statistical analysis (Duncan test) of data between treatments (Whole and empty fish covered in ice ) was significant (P
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
69
79
https://isfj.areeo.ac.ir/article_110173_7db98319680d1c621765031f1a2862d8.pdf
dx.doi.org/10.22092/isfj.2017.110173
Utilization of poultry by product meal as an alternative protein source for Huso huso within growth period
M. H.
Sayed Hassani
author
D.
Talebii Haghighi
author
M.
Hafeziehe
author
M. A.
Yazdani Sadati
author
H.M.
Pourali
author
H.
Yeganeh
author
text
article
2014
per
With regard to increasing the fish meal price, utilization of hygienic and analyzed poultry by product meal as an alternative protein source in diets for Huso huso within growth period were investigated. Six test diets isonitrogenous and isocaleric (contain 42% protein and 20 Mj kg-1 energy) were prepared. Fish meal was substituted by poultry by product at 20% (PBM20), 40% (PBM40), 60% (PBM60), 80% (PBM80) and 100% (PBM100) in base diet, respectively. A total of 180 Huso huso with average (±SD) weight of 107.89±5.9gr were stocked in 18 fiberglass tanks (Voloum:2000 lit,Temperature:20±2C0) and fed satiation to 18 weeks. No significant differences were detected between final weight (FW),weight gain (WG) and specific growth rate of fish fed (PBM0), (PBM20), (PBM40), (PBM60) and (PBM80) respectively. The Highest protein belonged to fish fed PBM20 (%16.97±0.36)with significant difference with PBM40 (% 15.41 ± 0.67), respectively. Body lipid fish fed PBM80 and PBM100 (%6.63±0.016) (%7.49±0.17) were significantly lower than other treatments. The Synchronic hepatosomatic index increased with increasing poultry by product in diets and highest rate were found in fish fed PBM 100 , but except PBM20, visceral index in fish fed other treatments was same. The result showed that up to 80% fish meal could be replaced by poultry by product with no adverse effect on growth and feed conversion ratio for Huso huso within growth period.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
81
96
https://isfj.areeo.ac.ir/article_103554_a86792eb9ae8e7b7ef726dc092448087.pdf
dx.doi.org/10.22092/isfj.2014.103554
Effect of fluoxetine on food intake and feeding behavior of Goldfish Carassius auratus
N.
Forsatkar
author
K.
Kukaram
author
M.A.
Nematolahi
author
text
article
2014
per
Serotonin is one of the monoamines that play an important role in food intake in mammalian species. The increase in serotonergic activity may reduce appetite. Fluoxetine as an active ingredient of Prozac is increased in serotonin content by blocking reuptake of it. Due to the release of these substances into the ecosystem, the role of fluoxetine on food intake and feed behavior of goldfish, Carassius auratus was investigated. In experiment 1, fish with 21-49 g weight divided in four groups of control: with no injection; saline injected; and two groups with 1 µg g-1 and 10 µg g-1 body weight fluoxetine. Animals were injected every other day for a total of 5 injections. Food intake calculated after each injection and fish reweighted at the end of the experiment to achieved weight changes. In experiment 2, 20 goldfish were selected in two groups of control and injected with 10 µg g-1 body weight fluoxetine to asses feed behavior test. Fish were injected every other day for a total of 5 injections and feed behavior was investigated after each injection. Food intake was significantly decreased after fluoxetine injections. In the Flu-10 group, low intake of food resulted in minimum weight gain among all treatments. Also, fluoxetine affected the feed behavior of goldfish and significantly was decreased in search and consumption of food. Results showed that this recently toxic environmental material can largely affect the food and weight parameters of goldfish.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
97
108
https://isfj.areeo.ac.ir/article_103555_c9b35a712836ffde04ea1337a1befb96.pdf
dx.doi.org/10.22092/isfj.2014.103555
The effects of Zataria multiflora on inhibition of polyphenoloxidase and melanosis formation in shrimp
(Litopenaeus vannamei)
E.
Nasiri
author
M.
Moosavi-Nasab
author
S. S.
Shekarforoush
author
M. T.
Golmakani
author
text
article
2014
per
Shrimp melanosis (black spot) is an important surface discoloration caused by polyphenol oxidase (tyrosinase) enzyme, which oxidizes phenols and leads to insoluble black pigments, the melanins. Sulphiting agents are widely used as melanosis inhibitors; but, the hazards related to sulphated foods, such as allergic reactions and severe disorders in asthmatic patients have created a necessity to find the effective natural alternatives. The current study was accomplished to assay the in vitro antityrosinase effect of Z. multiflora EO as well as its capability to retard the melanosis formation in shrimp during iced storage. According to GC/MS results, carvacrol, thymol and p-cymene were the major components of Z.multiflora EO, representing 50.8, 14.4 and 10.6, respectively. DPPH radical scavenging activity of EO was 0.8±0.02 mg/ml and 63.2% of tyrosinase activity decreased when EO with a concentration of 0.25% was applied. Furthermore, it has been observed that immersing the shrimps in1% EO aqueous suspensionretarded themelanosis formation in shrimp during 10 days of iced storage. It can be concluded that Z. multiflora EO could be used as an effective natural processing aid to increase the shrimp shelf-life during iced storage.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
109
118
https://isfj.areeo.ac.ir/article_103556_f633a3865112cc9124172e5f13e92ca5.pdf
dx.doi.org/10.22092/isfj.2014.103556
Some biological aspects of Mesopotamichthys sharpeyi in Shadegan Wetland, Iran
S.A.
Hashemi
author
R.
Ghorbani
author
F.
Kaymaram
author
S.A.
Hossini
author
G.
Eskandari
author
A.
Hedayati
author
text
article
2014
per
Some biological aspects of Mesopotamichthys sharpeyi were studied in Shadegan Wetland, Iran. Monthly samples were collected from five stations: Doragh, Rogabe, Khorosy, Salmane and Atish. A total of 437 specimens were measured from April 2013 to March 2014. Mean (±SD) (Range) length values for the male and female were calculated as 252±28 (95-357), 249±38 (115-374)mm respectively and mean (±SD) (Range) weight values were 218±31 (18-366 ),239±39(15-651)g respectively. The length-weight relationships were calculated as W=0.000006L3.11 (R2=0.85) for male, W=0.000005L3.14 (R2= 0.86) for female. The spawning occurred from April to July, and length at maturity (LM), weight at maturity (WM) and production per biomass (P/B) were calculated for male and female as LM=208, 220 mm WM=97, 133 g and P/B= 0.53, 0.50 per year, respectively.
*Corresponding author
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
119
128
https://isfj.areeo.ac.ir/article_110174_51cbc0d6b9df2bdfb49d241525486a0f.pdf
dx.doi.org/10.22092/isfj.2017.110174
The effect of dry salting on the shelf life of big mouth saury (Scomberoides commersonnianus)
Z.
Hadizadeh
author
N.
Mooraki
author
S.
Moini
author
text
article
2014
per
The present study investigated the effects of salting process on shelf life of big mouth saury (Scomberoides commersonnianus) filets. To determine the quality, chemical experiments including crude protein, peroxide value, crude lipid, ash-salt and moisture measurements were conducted at time intervals of 0, 15, 30, 90, 150 and 190 days. Moreover, fatty acid's profile was measured at time intervals of 0, 90 and 190 days. Mean (±SD) crude protein, crude lipid, ash-salt and moisture contents in fresh fish were 20.07±0.85, 6.32±0.42, 2.17±0.2and 71.44±0.19, respectively, reaching 30.5±1.19 8±0.12, 17.6±0.1 and 43.9±0.91, respectively, after salting and storing at the ambient temperatures for 190 days. The mean (±SD) peroxide was 0.05±0.01 meqO2 kg-1, and in fresh fish reached to 2.5±0.05 meqO2 kg-1 at the end of storage time as salted products. Variations in all of these factors were significant. According to the obtained results, the best time period for storing is 90 days. In the present study, 21 fatty acids were recognized. Total saturated, and unsaturated fatty acids in fresh samples (time 0) were 53.41 and 54.47%, respectively, and reached 56.99 and 39.92 at the end of storage time as the salted product. The result of the organoliptic showed that the new method of the dry salting give a better quality to the product in comparison with the traditional method of dry salting.
*Corresponding author
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
23
v.
3
no.
2014
129
138
https://isfj.areeo.ac.ir/article_110175_780bcd12615ac26f15663826902ef8b5.pdf
dx.doi.org/10.22092/isfj.2017.110175