حسین
آذری
author
رضا
قربانی واقعی
author
مهران
جواهری بابلی
author
text
article
2013
per
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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no.
2013
1
22
https://isfj.areeo.ac.ir/article_110082_926011c14d74b654f77c4b804109a5de.pdf
dx.doi.org/10.22092/isfj.2017.110082
Effect of Zataria multiflora essential oils on rainbow trout (Oncorhynchus mykis) complement component activity and lysozyme
M.
Soltani
author
T.
Zarifmanesh
author
S.J.
Zorriehzahra
author
text
article
2013
per
Influence of dietary administration of Zataria multiflora Bioss was evaluated on complement
component activity and lysozyme of rainbow trout (Oncorhynchus mykiss) to determine stimulatory
effect of essential oils. Fish weighting 100±10 g were fed with different doses of 10, 20 and 40 ml/kg
food for a period of 10 days. Alternative Complement Activity (CH50%) and serum lysozyme activity
were measured on days 1, 8, 15 and 29 after the essential oils administration. The obtained results
showed that for first and eighth days of sampling, the complement component mean in treatment
groups were significant higher than control group, while negligible difference is shown between
treatment groups. For fifteenth and twenty ninth days of sampling, no significant difference was seen
in the complement component mean among all examined groups. Also, the results of experiment show
that for all of days of sampling, essential oils of Zataria multiflora Bioss have no effect on serum
lysozyme activity.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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https://isfj.areeo.ac.ir/article_110083_f68e4a52484a9235f5df2d598d9befec.pdf
dx.doi.org/10.22092/isfj.2017.110083
Identification, abundance and biomass of benthic communities in south east coasts of the Caspian Sea (Golestan Province)
S.
Sharbati
author
R.
Akrami
author
S.
Yelghi
author
J.
Mirdar
author
Z.
Ahmadi
author
text
article
2013
per
The frequency and distribution of benthic species in the south east coast of Caspian Sea (proposed
site for cage and pen culture) were studied. Sampling was carried out in 2 water depths (1, 5) meters
and 12 stations were sampled in each depth using VanVeen sampler. Totally, 11 taxa were identified:
Pyrgulidae,Ampharetidae, Neritidae, Nereidae, Cardidae, Gammaridae, Naididae, Balanidae,
Foraminifera, Ostracoda and Cumaceae. The most abundant taxa were Gastropoda (66.36%),
Foraminifera (15.66%), Polychaeta (14.09%) and Bivalvia (1.65%) respectively. The maximum and
minimum biomass was 164.1 g/m in summer and 6.56 g/m in spring. Depth, substratum, season,
season-depth interaction, season- substratum âdepth interaction had significant effects on biomass and
had no significant effect on abundance.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
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https://isfj.areeo.ac.ir/article_110084_8f25e4e8aa92c35667f4bd3dc6b2b7d6.pdf
dx.doi.org/10.22092/isfj.2017.110084
Investigation of anesthetizing effect of tobacco (Nicotiana tabacum) aqueous and alcoholic extract on Rainbow trout (Oncorhynchus mykiss)
D.
Zargham
author
M.
Sharifrohani
author
I.
Falahat Naserabad
author
T.
Bashti
author
text
article
2013
per
The main goal of anesthesia in rainbow trout is decrease mobility and reduce stress of fish while
breeding, and also facilitate operation such as manipulation to Tagging, Biometry, Sorting, Inspection
and Sampling for pathology test. In the present study, Aqueous and alcoholic extract of tobacco plant
(Nicotiana tabacum) for Anaesthetizing Rainbow trout (Oncohrynchus mykiss) in 3 weight groups: fry
(less than 80 g), growing(about 200 g) and breeding(larger than 1500g) were used. The effective dose
of tobacco in treatments 200, 400, 800 and 1600mg/lit in aqueous and 0.7, 1, 1.7 and 3.5mg/lit in
alcoholic extract were tested. Totally, we can say that tobacco extract Anaesthetizes the rainbow trout
in less than 3 minutes and recovery time is less that 5 minutes. Regarding to anesthetizing time,
recovery time and fish relaxing during anesthesia, aqueous extract applies more gentle anesthesia in
rainbow trout, and dose 200 to 800 mg/lit of aqueous extract were more successful. Also Regarding to
significant correlation between doze and Anesthetics extremity and recovery time (P
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
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https://isfj.areeo.ac.ir/article_110085_c78040fa3e178503bf1e6c27366f207e.pdf
dx.doi.org/10.22092/isfj.2017.110085
Effect of Dietary Protein and Energy Levels on the Growth and Body Composition of Juvenile Orange-spotted Grouper, Epinephelus coioides
J.
Marammazi
پژوهشکده آبزی پروری جنوب کشور، اهواز، صندوق پستی: 866-61645
author
E.
Pagheh
پژوهشکده آبزی پروری جنوب کشور، اهواز، صندوق پستی: 866-61645
author
Z.
Mokhaiier
موسسه تحقیقات علوم شیلاتی تهران صندوق پستی: 6116-14155
author
text
article
2013
per
To evaluate the protein and energy requirement of orange-spotted grouper, Epinephelus
coioides fingerlings, nine experimental diets including 3 levels of crude protein (40%, 50%
and 60%) and 3 levels of digestible energy (DE, 14, 16 and 18KJ.g-1), were tested. Triplicate
300 L circular tanks provided with flow-through water system (2L. min-1) and air stone and
each stocked with 20 juvenile of grouper fish (16.79±0.4g) were applied to adopt an
experimental test for 8-weeks rearing period. The stocked fishes were fed experimental diets
to approximate satiation, twice a day. Results showed that the survival rate, hepatosomatic
index (HSI) and condition factor (CF) were independent of the dietary treatments, but final
weight, weight gain, specific growth rate(SGR), feed efficiency (FE), feed conversion ratio
(FCR), protein efficiency ratio (PER) and daily feed intake (DFI) were significantly affected
by protein and digestible energy levels (P0.05) between the treatments. viscerosomatic index(VSI) increased
significantly with the increasing of diet energy level. Body lipid, ash and moisture contents
were affected by dietary protein and energy levels. Body lipid content increased with
increasing dietary energy levels, but carcass ash and moisture decreased. Body protein content
was not affected by dietary energy level, but significantly increased when protein increased
from 40% to 50% at same energy levels (P
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
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https://isfj.areeo.ac.ir/article_110086_17c0025d4b39cab4b02f87839653e5fc.pdf
dx.doi.org/10.22092/isfj.2017.110086
Changes in stocks of anchovy kilka, Clupeonella engrauliformis in Iranian coastal zone of the Caspian Sea (1995-2011)
H.
Fazli
پژوهشکده اکولوژی دریای خزر، ساری صندوق پستی 916
author
A.A
Janbaz
پژوهشکده اکولوژی دریای خزر، ساری صندوق پستی 916
author
F.
Keymaram
موسسه تحقیقات علوم شیلاتی ایران، تهران صندوق پستی: 1661-14155
author
SH.
Abdolmaleki
پژوهشکده آبزی پروری آبهای داخلی، بندر انزالی، صندوق پستی: 66
author
K.
Khedmatei
پژوهشکده آبزی پروری آبهای داخلی، بندر انزالی، صندوق پستی: 66
author
text
article
2013
per
The main objective of the present study was qualitative and quantitative assessment of
anchovy stock, Clupeonella engrauliformis in Iranian waters of the Caspian Sea during (1995-
2011). Over this period, total catch of anchovy kilka increased from 32000 tonnes in 2005 to
67000 tonnes in 1999 and decreased sharply to 80 tonnes in 2011. In the years 1995-1999,
catch per unit effort (CPUE) was fairly constant (2.92-3.52 tonnes/(VesselsÃNights) which
fell to 0.01 tonnes/(VesselsÃNights) in 2011. Total biomass and spawning stock biomass
(SSB) had the same trend as CPUE. During 1995-1999, total biomass of anchovy kilka ranged
between 158500-185900 tonnes which dropped to 2000 tonnes in 2007. The SSB showed a
downwardtrend from 105500 tonnes in 1997 to 1950 tonnes in 2007. In age compositions of
the catch, age 3+ was the largest age group. From 2001 to 2003, age 2+ predominated. Finally,
number of young specimens declined and age 6+ and 7+ groups were predominated in the last
years. Present study indicated that the situation of the anchovy kilka is critical. A coordinated
international effort is needed to provide implementation of sustainable management and
responsible fishing activities in the whole Caspian Sea.
*Corresponding author
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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no.
2013
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66
https://isfj.areeo.ac.ir/article_110087_723c6a887ee704852d41d465519886e5.pdf
dx.doi.org/10.22092/isfj.2017.110087
حمید
قاسمی
author
محمود
رامین
author
text
article
2013
per
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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no.
2013
67
74
https://isfj.areeo.ac.ir/article_110088_98fd836ebbfdbc319f7c2879fe698cdd.pdf
dx.doi.org/10.22092/isfj.2017.110088
Determination of growth parameters of the king soldier bream (Argyrops spinifer), using the backcalculation method and otolith reading data in coastal waters of Bushehr Province, Persian Gulf
M.
Ghanbarzadeh
author
N.
Mahboobi Soofiani
author
Y.
Keivany
author
S.
Asadollah
author
S.A.
Taghavi Motlagh
author
text
article
2013
per
Age and growth of the king soldier bream, Argyrops spinifer (Forsskal 1775) in the Persian
Gulf (Bushehr coasts) were investigated. A total of 622 samples were obtained from June
2010 to May 2011. Total length (TL) of fish ranged from 13.5 to 64.2cm (females) and 15 to
61.2cm (males). Male and female fish age ranged between 2+ to 25+ and 2+ to 21+ years,
respectively. Values of the von Bertalanffy growth function were calculated in females, males
and total population by using the backcalculation method and otolith reading data. The values
of these parameters according to otolith reading data were K=0.061, Lâ=82.10cm (TL), t0=-
2.39 years for females and K=0.082, Lâ=67.90cm (TL), t0= - 2.88 for males and K=0.065,
Lâ=75.50cm (TL), t0=-3.21 for total population and according to backcalculation method
were K=0.088, Lâ=76.37cm (TL), t0=-0.329 years for females and K=0.101, Lâ=65.71cm
(TL), t0=-0.168 for males and K=0.094, Lâ=74.55cm (TL), t0=-0.191 for total population. The
results of the study provide the direction for the future management models for the continued
sustainable exploitation of this species in the Persian Gulf region.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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2013
75
84
https://isfj.areeo.ac.ir/article_110089_364d2fdc430c82bdad872320755dd105.pdf
dx.doi.org/10.22092/isfj.2017.110089
Effects of dietary carbohydrate levels on growth, survival and body composition in Caspian Kutum (Rutilus frisii kutum, Kamenskii, 1901)
S.
Mohammadzadeh
author
H.
Noverian
author
H.
Ouraji
author
B.
Falahatkar
author
text
article
2013
per
This study was carried out to evaluate the effects of dietary carbohydrate levels on growth
performance and body composition of juveniles Caspian Kutum (Rutilus frisii kutum). Fish
with initial average weight of 0.8±0.2g (±SD) were fed five isoproteic and isenergic
formulated diets with different carbohydrate levels including 15, 20, 25, 30 and 35% in
triplicate groups, 4 times daily according to their satiation for 10 weeks. Results showed that
increasing the level of carbohydrate to 35% significantly increase some growth indices such
as final weight (Wf), weight gain (WG), body weight increase (BWI), specific growth rate
(SGR) and protein efficiency ratio (PER). In addition, result of body composition analysis
showed that the increase in carbohydrate level lead to increase of protein level. So, the highest
amount of protein was observed in fingerlings fed with diet containing 35% carbohydrate. In
conclusion, the results indicate that increase in carbohydrate levels from 15 to 35% have not
any negative effect on growth parameters and therefore, the level of 35% carbohydrate in the
diet of 1-2g Caspian Kutum fingerlings can lead to better efficient in protein and desirable
growth.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
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no.
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94
https://isfj.areeo.ac.ir/article_110090_d06fcc024bd334b55e7b61088c6b739d.pdf
dx.doi.org/10.22092/isfj.2017.110090
Histopathological Effects of Mercuric Chloride on Kidney and Intestine of Persian Sturgeon, Acipenser persicus fry
S.
Mosafer Khoorjestan
دانشکده منابعطبیعی و علومدریایی دانشگاه تربیت مدرس، مازندران، نور- ایران.
author
S.
Khodabandeh
دانشکده منابعطبیعی و علومدریایی دانشگاه تربیت مدرس، مازندران، نور- ایران.
author
Z.
Khoshnood
دانشکده منابعطبیعی و علومدریایی دانشگاه تربیت مدرس، مازندران، نور- ایران.
author
text
article
2013
per
To study the effects of mercuric chloride on kidney and intestine tissue of Persian Sturgeon,
Acipenser persicus fry, fish exposed freshwater contain 15 ppb of mercuric chloride and control group
were kept in non polluted freshwater for 48 hours. Histological studies of the processed tissues
showed marked histological alterations than the control group that exposed only to freshwater. The
pathologic lesions of the kidney included severely epithelial cell necrosis, lesion of epithelial cells
from the basement membrane in the proximal and distal tubules. Proximal tubule also showed
inflammation and incorporation of brush borders. Decrease of lymphoid and hematopoietic tissue
observed. Pathologic lesions of intestine tissue were showed the epithelial necrosis, shrinkages of
secretory follicule, lesion of epithelial microvilli in intestine. Results showed that low concentration
of mercuric chloride can pose undesirable alternation in Persian Sturgeon fry, an economic and
endangered species of the Caspian Sea.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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no.
2013
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104
https://isfj.areeo.ac.ir/article_110091_80d2e89ebd885602ee8bd5e8e038cee9.pdf
dx.doi.org/10.22092/isfj.2017.110091
The effects of dietary selenium on the oxidative stress of beluga, Huso huso, fed high copper
M.
Mohseni
author
A.M.
Sotudeh
author
text
article
2013
per
The effects of dietary selenium (Se) were investigated on the oxidative stress of beluga,
Huso huso, fed high dietary copper (Cu). The basal diet with high Cu (50mg Cu/kg) was
supplemented with low (0mg Se/kg, HCuLSe), adequate (11mg Se/kg, HCuNSe) and 5Ã
adequate (55mg Se/kg, HCuHSe) of Seandfed to fish with initial body mean (±SD) weight
14.96 ± 1.39g for 12 weeks. Basal diets containing adequate Se (11mg Se/kg) and adequate
Cu (10mg Cu/kg) were used as controls (NCuNSe). Weight gain, specific growth rate and
condition factor for fish fed the HCuHSe diet was significantly lower than fish that fed the
other diets. Se and Cu content was positively correlated with dietary Se and Cu
concentrations. Hepatic Se concentration was higher in fish fed the HCuHSe diet than fish
that fed other diets. Thiobarbituric acid-reactive substances (TBARS) were significantly
lower. Whereas, Glutathione peroxidase activity activities were significantly higher in liver
tissue of fish fed HCuHSediet than those fish fed other diets. Plasma total immunoglobulin
concentration and lysozyme activity were higherin fish fed the NCuNSe diet, followed by fish
fed the HCuNSe diet, and were lower in fish fed the HCuHSe diet. The results indicated that
optimum levels of Cu and Se ingestion induced an oxidative stress in beluga and improved
their immune response. High dietary Se (5Ãadequate) supplementation reduced the oxidative
stress and depressed the immune response of the fish.
*Corresponding author
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
21
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no.
2013
105
114
https://isfj.areeo.ac.ir/article_110092_8d55353a21a4c0e9f4ccb446408bef14.pdf
dx.doi.org/10.22092/isfj.2017.110092
Effect of reused water on growth index and survival rate of the Rainbow trout (Oncorhynchus mykiss)
A.
Nekuie Fard
author
H.
Hossainzadeh Sahhafi
author
A.A.
Motalebi
author
A.H.
Rastiannassab
author
داریوش
Azadikhah D.
author
B.
Mostafazadeh
author
text
article
2013
per
This research was carried out to determine water reuse effects on growth indices of rainbow trout.
For this reason 4 groups including: Control (with use of 100% fresh water), Test 1(rearing with use of
30% recycled water (with physical filtration and 10% fresh water), Test 2 (using of 10% recycled
water (with physical filtration and 30% fresh water), Test 3(using of 100% recycled water) was done.
Fish stock of each groups were done with 62 fish in each square meter and average (±SD) weight
(15±1.5). The Growth index, SGR, CF, FCR, SR between different groups in each month was
calculated and determined. In this research comparing the average of monthly growth increasment
between different groups indicated that significant decrease of growth in test groups with control
group as well as groups 1 and 2 with 3 groups. There were no significant differences in this factor
between groups 1 and 2. FCR, SR and MR showed a significant difference between different groups.
These results showed with increasing the replacement of water in rainbow trout culture ponds with
recycled water cased significant decrease in SR and CF and significant increase in MR and finally
decrease in production and density per unit area.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
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124
https://isfj.areeo.ac.ir/article_110093_9c3a5efd256fc315a514e5bbe27c73fc.pdf
dx.doi.org/10.22092/isfj.2017.110093
Study on Bentic organisms of Ghazal Ozan River in Zanjan Province
Navan
Maghsoodi M.
author
text
article
2013
per
Ghazal Ozen is one of the two main Caspian Sea catchments entering to the Sefidrood Dam.
Sampling was done within 45 days from October 2009 till October 2011 on 3 stations. Benthic
samples were fixed with %4 fomaline and were identified to the genus or species levels and
investigated using to the Hilsenhoff biogogical index. In this study, 20 genera 7 orders were
identified chironomidae as dominant family. Hilsenhoff Biological Index (HBI) for the 9 studied
stations, were respectively, 5.4, 6.3, 5.46, 5.11, 5.13, 4.13, 4.25, 4, 4.23.The biological status of
rivers based on HBI data was in the range of very good level. In the study, Chironomidae (30%),
caenis sp. (16%), Baetis sp.(12%), Hydropsyche sp. (13%), Simmulium sp. (11%), Tabantus atratus
and Sericostoma sp. (2%) and remaining benthos (14%) in Ghazan Ozan River. The results showed
that the presence of organic matter pollutant has reduced oxygen levels and also affected the biota
in Ghazal Ozan River.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
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2013
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138
https://isfj.areeo.ac.ir/article_110094_6813bb10e3d5f977f87afc2f72086131.pdf
dx.doi.org/10.22092/isfj.2017.110094
Diet composition, Trophic level estimation and Food consumption rate of Saurida tumbil (Teleostomi/Synodontidae) in the Bushehr coastal waters, Persian Gulf, Iran
A.
Vahabnezhad
author
S.A.
Taghavi Motlagh
author
Javad
Shaebani
author
text
article
2013
per
In order To increase the knowledge of Saurida tumbil biology and ecology, an
investigation of food habits of this species was conducted from October 2010 to September
2011 in Bushehr coastal waters, Persian Gulf. 485 fish stomach contents were studied. In
this study the frequency of occurrence of prey, feeding intensity index, the index is empty
stomachs, the mean Trophic levels and the rate of food consumption was calculated. Total
three major taxonomic categories were identified in the stomach contents of this species and
were weighted including arthropods (crustaceans higher crustaceans), mollusks
(Cephalopod) and Teleostei and the results show that Teleostei was the most abundant prey
item which including the family Carangidae, Nemipteridae, Synodontidae, Mullidae,
Sphyraenidae, Leiognathidae , Haemulinae and Trichiuridae. The results showed that the
highest intensity of feeding occurs in the fall and lowest in summer. S. tumbil also shows
cannibalistic behavior in ecosystem. The mean trophic level for S. tumbil was estimated 4.64
for the first time in Persian Gulf, indicating that S.tumbil is largely carnivore and has a high
rate of food consumption.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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2013
125
150
https://isfj.areeo.ac.ir/article_110095_6833caa12b0abf46e7ba042e96a5e170.pdf
dx.doi.org/10.22092/isfj.2017.110095
Parasitic infection in Hemiculter lucisculus, Liza auratus and Gasterosteus aculeatus of the Zardi River(Mazandaran)
M.
Taghavi
author
B.
Mokhayer
author
A.A.
Saeedi
author
H.
Mosavi
author
text
article
2013
per
A total of 76 specimens of three fish species including: Hemiculter lucisculus (41), Liza
auratus (16) and Gasterosteus aculeutus (19) were caught using handnet from 3 station in
Zardi River in the south-eastern costal of Caspian Sea at autumn and winter 2009 and spring
2010. The fishes were transported alive to the laboratory of Shahid Rajaee Site. They were
killed by cutting their spinal cord and their length and weight were measured. The eye, skin,
gill, digestive system, muscle and other organism's of the specimens were examined for
parasite infestation. Thirteen parasites species were identified. Ichthyobodo necator was
collected from skin of Hemiculter lucisculus and Liza auratus. Ichthyophthirius multifiliis was
collected from skin or branchia of Liza auratus and Gasterosteus aculeutus, Chilodonella sp.
was collected from skin of Gasterosteus aculeutus, Trichodina sp. from skin and branchia of
Hemiculter lucisculus, Liza auratus and Gasterosteus aculeutus, Tetrahymena sp. was
collected from skin of Gasterosteus aculeutus , Myxobolus sp. from branchia of Hemiculter
lucisculus, Dactylogyrus sp. from branchia of Hemiculter lucisculus and Gasterosteus
aculeutus, Gyrodactylus sp. from skin or branchia of Gasterosteus aculeutus, Discocotyle sp.
and Diplozoon sp. from branchia of Hemiculter lucisculus, Diplostomum spathaceum from
eyes of Hemiculter lucisculus, Saccocoelium obseum from intestine of Liza auratus and
Corynosoma stromosum from abdominal cavity of Gasterosteus aculeutus. In present study
Hemiculter lucisculus was as a new host for Ichthyobodo necator, Myxobolus sp., Discocotyle
sp. and Diplozoon sp. also except Saccocoelium obseum from Liza auratus and Corynosoma
stromosum from Gasterosteus aculeatus, the other parasites were reported for the first time
in Iran.
*Corresponding
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
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2013
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156
https://isfj.areeo.ac.ir/article_110096_33b39e862ec7f8539880f45b72167085.pdf
dx.doi.org/10.22092/isfj.2017.110096
Short term preservation of spermatozoa of rainbow trout (Oncorhynchus mykiss)
M.
Pourkazemi
author
A.
Shakibi Daryakenari
author
M.R.
Kalbasi
author
H.
Abdolhay
author
S.
Baradaran Noveiri
author
text
article
2013
per
Short term preservation of spermatozoa of salmonid fishes for practical use, increasing of
reproduction potentials and reducing of expenses, has an important role in hatcheries. In this
study spermatozoan motility and fertilization rate of 29 commercially cultured rainbow trout
were examined. Semen was collected by abdominal stripping at the volume of 4.49±1
(mean±SD) ml and sperm concentration was determined by heamocytometer following 1:600
dilution with saline solution (0.9% NaCl). To find the effects of preservation time on
fertilization capacity, samples were diluted with extender (Glucose 54gL-1, Tris 0.5gL-1,
DMSO 9% , Egg yolk 20%) and held at 3 different cool conditions : room temperature
(+15°C), refrigerator (+5°C) and crashed ice (+ 1-2°C) and sperm motility followed up each
30 mins until complete ceasation. Fertilization, hatching and larval stage rate were determined
after 24, 48 and 72 hours. Results showed that the motility of sperm samples held at 15 °C
and 5°C were completely ceased after 2 and 24 hours respectively. However it lasted up to
144 hours at1-2°C.Fertilization, hatching and larval stage rates of sperm samples hold at 1-
2°C were 68.45% , 62.1% and 58.2% (after 24 hours), 53.2% , 43.2% and 40.9% (after 48
hours) and 36.6% , 31.6% and 23.7% after 72 hours, respectively, and all showed a
significant decrease compared with control (90.32%, 83.27% and 81.12% respectively).
Comparing the results showed that holding samples in test tubes at 1-2°C is the best method
for short term storage of rainbow trout spermatozoa.
Iranian Scientific Fisheries Journal
Agricultural Research,Education and Extension Organization
1026-1354
21
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dx.doi.org/10.22092/isfj.2017.110097