ORIGINAL_ARTICLE
Concentration of heavy metals (Cd, Cr, Zn, and Pb) in muscle and liver tissues of common carp (Cyprinus carpio L., 1758) from coastal waters of Golestan Province
Concentrations of four heavy metals (Cd, Cr, Zn, and Pb) in the common carp (Cyprinus carpio)
were assessed in winter, spring and summer seasons of the years 2008. Four coastal areas in the west
and east, Gomishan Marsh and Gorgan Bay including 11 sites were sampled. After biometrical
measurements of the fish, specimens of muscle (104) and liver (36) tissues were immediately frozen
and transferred to laboratory for assessment of heavy metal concentration. Dry ash method and atomic
absorption spectrometer (AAS) of Perkin Almer (400 model, German) were used to assess metals
concentration. In the process, analysis of variance (ANOVA) and Tukey-test were implemented.
Heavy metals concentration in kidney tissue was higher than muscle tissue. In carp muscle tissue,
level of zinc was highest and the next categories were those of lead, cadmium and chromium,
respectively. A significant difference was observed between chromium concentrations in muscle tissue
of Cyprinus carpio with sex (P
https://isfj.areeo.ac.ir/article_109955_08759ecdac0fa6899ca77df528c2a53d.pdf
2011-05-22
1
10
10.22092/isfj.2017.109955
heavy metals
Cyprinus carpio
Golestan Province
Gh.A.
Bandani
bandany_a@yahoo.com
1
LEAD_AUTHOR
H.A.
Khoshbavar Rostami
2
AUTHOR
S.
Yelghi
3
AUTHOR
M.
Shokrzadeh
4
AUTHOR
H.
Nazari
5
AUTHOR
ORIGINAL_ARTICLE
Effects of dietary nucleotides levels on whole body composition of orange spotted grouper (Epinephelus coioides)
This research was carried out for 10 successive weeks (2 weeks for adaptation and 8
weeks for culture) in summer 2008 at Abzisytan Shrimp Hatchery Center in Bushehr
province. The effects of dietary nucleotides at 5 levels including 0.0% (control), 0.15%,
0.25%, 0.35% and 0.5% on the growth performance and whole-body composition of
Epinephelus coioides was investigated. Juvenile fish specimens with average weight of
10.70±0.29g were fed by experimental diets for 8 weeks. The trial was carried out in 300 liter
fiberglass tanks. The highest protein content was observed in 0.15% diet nucleotide and the
highest fat in 0.15% and 0.25% diet nucleotide which showed significant difference with the
control group (P
https://isfj.areeo.ac.ir/article_109956_40cda517e95c9eb0a340f95e64b604e0.pdf
2011-05-22
11
20
10.22092/isfj.2017.109956
muscle
food
Growth
Epinephelus coioides
B.
Bahmani
mahmoudbahmani@ymail.com
1
LEAD_AUTHOR
A.
Zariffard
2
AUTHOR
M.
Khodadadi
3
AUTHOR
N.
Mahmoudi
4
AUTHOR
A.
Ojeefard
5
AUTHOR
ORIGINAL_ARTICLE
Production of single cell protein from stickwater of kilka fish meal factory using Lactobacillus acidophilus and Aspergillus niger
We investigated production of single cell protein (SCP) from stickwater of kilka fish meal
factory as medium using Lactobacillus acidophilus and Aspergillus niger. Stickwater was
used instead of the standard media of bacterium and fungus in a batch culture method.
Amount of biomass, COD, RNA and protein in the bacterium and fungus in control and
stickwater treatments were investigated. In maximum growth time, amino acids profile of the
bacterium and fungus were measured and compared between treatments. Bacterial biomass
production in the control and stickwater treatments were 3.16 and 5.12g/l, COD reduction was
33270 and 53330mg/l, the measured RNA were 15.27% and 15.04%, the amount of protein
were 71.13% and 68.37%, respectively. The difference between bacterium and fungus
biomass production was slight. We found that the amount of the fungus biomass in control
and stickwater were 6.31 and 7.28g/l, COD reduction were 47800 and 55200mg/l, RNA was
9.36% and 9.09%, the amount of protein were 51.36% and 48.66%, respectively. In both
bacterium and fungus, the maximum and minimum amount of amino acid of the control and
stickwater was glutamic acid and methionin. The amount of methionin in bacterium was not
different with fish meal and FAO reference and in fungus was a little lower than FAO
reference. According to the results, application of pure stickwater was suitable for production
of Lactobacillus acidophilus and Aspergillus niger.
https://isfj.areeo.ac.ir/article_109957_4507a48a3d1f51ed8181453d48917716.pdf
2011-05-22
21
32
10.22092/isfj.2017.109957
biomass
Microorganism
Stickwater
Amino acids
S.
Bebekam
1
AUTHOR
A.M.
Abedian
edian@modares.ac.ir
2
LEAD_AUTHOR
H.
Younesi
3
AUTHOR
ORIGINAL_ARTICLE
Effects of plant proteins as food on growth performance, carcass quality and plasma biochemical parameters of Beluga juvenile (Huso huso)
The possibility of replacing fish meal with plant protein sources (soybean meal and corn
gluten) for beluga (initial body mass 159.55±2.14g) was studied in autumn 2009.
Experimental feeds with 46.5% of crude protein, GE: 5.4 Cal g-1 in four replacement levels: 0,
270, 450 and 660 gr kg1 were prepared and fish were fed on the diets for 60 days. Significant
differences were found in growth performance (P
https://isfj.areeo.ac.ir/article_109958_074ab6c54e6ee2e5cd6bf6ea63240dfd.pdf
2011-05-22
33
42
10.22092/isfj.2017.109958
Beluga
Corn Gluten
SOYBEAN MEAL
V.
Taghizadeh
vahid_taghizadeh54@yahoo.com
1
LEAD_AUTHOR
M.R.
Imanpoor
2
AUTHOR
R.
Asadi
3
AUTHOR
V.
Chamanara
4
AUTHOR
S.
Sharbati
s_sharbaty@yahoo.com
5
AUTHOR
ORIGINAL_ARTICLE
Qualitative assessment of organic pollution from aquaculture activities on benthic organisms using ABC (Abundance Biomass Curves) in Ghazale creek (Persian Gulf)
The present study was carried out to find the probable effects of marine fish cage culture
on benthic communities as pollutant and stress indicators. Also, the biotic health condition
was assessed using ABC index, in Ghazale creek, Khore-Mussa area in northwest of the
Persian Gulf.
Monthly sampling from four stations was conducted from June 2007 to March 2008.
Stations were selected according to distance from cages in Ghazale creek. The distances were
immediately under the cages, 50, 150 and 400m from the cages. At each station, three samples
for macrobenthos and one sample for sediment grain size analysis and total organic matter
(TOM) were collected by Van Veen grab with 0.0225m2 area. The range of total organic
matter percentage in sediments was (6.17-23.26) and the range of silt-clay percentage was
(4.76-97.47).
We found 12 macrobenthic orders and Polychaets (60.62%), Mullusca (19.67%), Crustacea
(16.49%) were the dominant groups. Macrobenthic abundance, biomass and diversity index
value under cage station were less than those 400m away from the cages. Capitella sp. as
opportunist species was dominant under cage station. This genus is introduced as the pollution
indicator in the area. The result of ABC index show that under the cages and distances 50 and
150m away from the cages have average pollution, while the 400m distance from the cage
(control site) is unpolluted.
https://isfj.areeo.ac.ir/article_109959_05508e1bbc8e35eb142f742649003392.pdf
2011-05-22
43
54
10.22092/isfj.2017.109959
cage culture
Macrobenthos
Pollutant
Capitell sp
Persian Gulf
N.
Jahani
jahani4563@yahoo.com
1
LEAD_AUTHOR
S.M.B.
Nabavi
2
AUTHOR
S.
Dehghan Madiseh
s_dehghan2002@yahoo.com
3
AUTHOR
S.R.
Seyed Mortezaie
4
AUTHOR
ORIGINAL_ARTICLE
The probiotic effects of dietary inactive yeast Saccharomyces cerevisiae var. ellipsoideus on growth factors, survival, body composition and intestinal microbiota of Beluga juvenile (Huso huso)
The probiotic effects of inactive yeast, Saccharomyces cerevisiae var. ellipsoideus was
studied on growth performance, survival and intestinal microbiota of beluga juveniles (Huso
huso). The study was done in complete randomize design that included feeding of beluga
juveniles with diets supplemented with 0 (control), 1, 2 and 5% yeast (4 treatments with 3
replicates). Beluga juveniles (11.40±0.56g) were randomly allocated in 12 oval tanks at a
density of 35 fish per tank and triplicate group were fed with experimental diets. At the end
of the trial, growth factors (final weight, weight gain, SGR, CF) as well as feed conversion
ratio (FCR), body composition (protein, lipid, ash, moisture) and intestinal microbiota (total
viable bacteria and Lactobacillus spp. levels) were determined. Our results confirmed that
juveniles fed on diet supplemented with 5% S. cerevisiae var. ellipsoideus had significantly
higher final weight, weight gain, specific growth rate (SGR) and lower food conversion ratio
compared to control and 1% treatment (P0.05). The study of body
composition showed no significant difference between treatments (P>0.05). Total viable
bacteria and Lactobacillus spp. count were significantly higher in 5% treatment compared to
control (P0.05).
https://isfj.areeo.ac.ir/article_109960_291352be7edfd7653b789aab282b031b.pdf
2011-05-22
55
66
10.22092/isfj.2017.109960
Feeding
Intestinal microbiota
Beluga
S.H.
Hoseinifar
hoseinifar@ut.ac.ir
1
LEAD_AUTHOR
A.R.
Mirvaghefi
2
AUTHOR
B.
Mojazi Amiri
bmamiri@ut.ac.ir
3
AUTHOR
H.A.
Khoshbavar Rostami
4
AUTHOR
M.
Poor Amini
5
AUTHOR
K.
Darvish Bastami
6
AUTHOR
ORIGINAL_ARTICLE
Daphnia pulex through physical and chemical factors
Regarding the key role and importance of Daphnia in aquaculture, particularly for survival
and adaptation of fishes in larval stages, a comprehensive study on hatching rate of Daphnia
ephippial eggs was implemented to obtain an optimum technique. The effects of abiotic
factors was investigated on the hatching response of Daphnia pulex diapausing eggs collected
from live food ponds in Cultivation and Breeding Centre of Gorgan. After isolation, the eggs
were kept in dry and wet conditions at 4°C for 2 months in darkness. Following the preincubation
period, the eggs in each group were subdivided into 2 parts and treated with 1%
NaOCl and distilled water. The effect of temperature levels (15 C, 20 C, 25 C) and
photoperiod levels (12L: 12D, 24L: 0D) were identified on the hatching of eggs in AdaM
medium during 15 days. Results indicated that the exposure of wet-kept eggs to 12-hour
photoperiod and 25 C without soaking in 1% NaOCl, caused the maximum rate and speed of
hatching.
https://isfj.areeo.ac.ir/article_109961_07217dc046bf00cf87efbd9cd16ba369.pdf
2011-05-22
67
76
10.22092/isfj.2017.109961
Aquaculture
Feed
LIVE FOOD
Daphnia pulex
S.
Haghparast
sarah_haghparast@yahoo.com
1
LEAD_AUTHOR
K.
Darvish Bastami
2
AUTHOR
ORIGINAL_ARTICLE
Shelf-life determination of tiger-toothed Croaker (Otolithes ruber) during flake ice storage
Quality of tiger-toothed croaker stored in flake ice was studied for 19 days by sensory, chemical (total volatile basic nitrogen (TVB-N)), (thiobarbituric values (TBA)), pH and microbial (total viable count, TVC) methods. Sensory scheme was modified according to the panelists perception and a specific schema was created for this fish. Sensory analyses showed that: "gills color and odor", "convexity of eyes", "peritoneum" and "elasticity of flesh" are the best sensory parameters. Results of sensory and microbial analyses had high correlation. Total viable count of flesh was 2.86 log cfu g-1 at first day and reached the limiting level of TVC (106) after 15 days of storage (P
https://isfj.areeo.ac.ir/article_109962_980a1c280a7ea47b4125300434331b6a.pdf
2011-05-22
87
96
10.22092/isfj.2017.109962
shelf-life
quality
Tiger-toothed croaker
Flake ice
S.
Sharifian
sharifian.salim@hotmail.com
1
LEAD_AUTHOR
M.S.
Mortazavi
2
AUTHOR
E.
Zakipour Rahimabadi
3
AUTHOR
A.
Arshadi
4
AUTHOR
ORIGINAL_ARTICLE
Concentration of heavy metal (Cd, Pb, Ni, Hg) in muscle, gill and liver tissues of Barbus xanthopterus in Karoon River
We determined bioaccumulation of heavy metals (Cd, Pb, Ni and Hg) in the muscle,
gill and liver organs of Barbus xanthopterus in summer 2009 in Karoon River, south west
of Iran. Gill net was used to sample 48 random size fish specimens from Karoon River
downward of Ahwaz city (Shekariyeh 3 village). The tissues were extracted and the elements
were determined using atomic absorption spectrophotometer. Concentrations of heavy
metals Cd, Pb, Ni , Hg in muscle tissue of Barbus xanthopterus were 1.679, 2.37, 1.16,
1.29mg/kg dry wt and in gill were 2.17, 2.92, 1.44, 1.34mg/kg dry wt and in liver were
1.92, 2.79, 1.31, 1.43mg/kg dry wt, respectively. All the results were statistically significant
(P 0.05). The concentrations of heavy metals were higher than standards of the World
Health Organization (WHO) in the muscle tissue of Barbus xanthopterus in Karoon
River.
https://isfj.areeo.ac.ir/article_109963_1e14aebd673f19f95f9dee90bb1fb9c0.pdf
2011-05-22
97
106
10.22092/isfj.2017.109963
Pollution
Barbus xanthopterus
Karoon River
Khuzestan Province
A.
Askary Sary
1
AUTHOR
M.
Khodadadi
2
AUTHOR
M.
Mohammadi
40_mohammadi@yahoo.com
3
LEAD_AUTHOR
ORIGINAL_ARTICLE
Tracking of Hepatopancreatic parvo-like virus (HPV) disease in Litopenaeus vannamei of the hatcheries in the Bushehr Province
Presence of hepatopancreatic parvo-like vines (HPV) disease was assessed from June until
October 2009 in Litopenaeus vannamei hatcheries and grow-out farms of the Bushehr
province. Samples were collected from 6 hatcheries and 6 grow-out farms located in coasted
areas. From each hatchery, 100 PL samples with average age PL5-PL8 and 20-30 samples
from each grow-out farm with average age 105 to 120 days were collected. The samples were
divided into three groups one used for gross sign and wet mount with Gimsa, the second
group was preserved in Davidson Fixative and used for histopathology and the third group was
fixed in ethyl alcohol 95% and used for polymerase chain Reaction (PCR). In gross sign 30%-
40% of the shrimp showed different sizes and some were smaller than the others. In the wet
mount group with Gimsa staining of hepatopancrease, the inclusion body with basophilic color
was seen. The histopathology indicated that the hepatopancreatic cell was infected and the
basophilic inclusion body observed in many samples. The PCR examined with IQ 2000 Kit
was negative. The rate of infection (ROI) was 1.1% for hatcheries and 32% for grow-out
farms.
https://isfj.areeo.ac.ir/article_109964_74239ff9a4a3fb59b12a675dbcd448e6.pdf
2011-05-22
107
118
10.22092/isfj.2017.109964
Litopenaveus vannamei HPV Gross Sign
Histopathology PCR
T.
Ghaedi
tghaedi@gmail.com
1
LEAD_AUTHOR
M.
Afsharnasab
2
AUTHOR
A.M.
Koosarinejad
3
AUTHOR
Gh.H.
Mohammadi
4
AUTHOR
ORIGINAL_ARTICLE
Temporal and spatial distribution of fish larvae in Khur-e-Mussa Channel (Khuzestan-Persian Gulf)
Temporal and spatial distribution of fish larvae population in eastern and western parts of
Khure e Mussa channel in Khuzestan province (Persian Gulf) was studied during 2007-2008.
Monthly samples were collected by 300μm mesh size plankton net during which 9440 larvae
were collected. Totally, 22 families were identified including Bregmacerotidae,
Callionymidae, Carangidae, Clupeidae, Cepolidae, Cynoglossidae, Gobiidae, Leiognathidae,
Mugilidae, Platycephalidae, Scaridae, Sciaenidae, Scatophagidae, Scorpaenidae, Sillaginidae,
Soleidae, Sparidae, Stromateidae, Syngnathidae, Engraulidae, Trichiuridae, Triacanthidae.
The most frequent families were Gobiidae (22.26 ), Clupeidae (17.61 , and Sparidae
(8.77%), respectively. The highest densities of larvae were observed in beginning of spring.
Two peak of population was observed. The first one in early spring (April) and the second
peak was in mid-summer (August and September). Cluster analysis based on average annual
abundance of 12 important families classified stations into three groups with 70% similarity.
The results of cluster analysis separated western stations 1, 2 and 3 from others. Higher
densities of fish larvae in the western coast of Khur-e-Mussa channel showed that western
parts better for nursery grounds for the studied fish larvae
https://isfj.areeo.ac.ir/article_109965_4c34b08b65466eb53573cce42e48020c.pdf
2011-05-22
119
128
10.22092/isfj.2017.109965
Fish larvae
plankton
biosystematic
Khur-e-Mussa
Persian Gulf
E.
Koochaknjad
koochaknejad@gmail.com
1
LEAD_AUTHOR
S.
Dehghanmadiseh
s_dehghan2002@yahoo.com
2
AUTHOR
A.
Savari
savari53@gmail.com
3
AUTHOR
Gh.
Eskandari
4
AUTHOR
N.
Sakhiee
nsakhaee@yahoo.com
5
AUTHOR
ORIGINAL_ARTICLE
Effects of feeding frequency on growth, survival and water quality of rearing tanks of the Indian white shrimp (Penaeus indicus)
The effects of feeding frequency on growth performance, feed conversion ratio and
survival of post-larvae Indian white shrimp, Penaeus indicus were evaluated in a 56-day
study. The study was conducted in sixteen fiberglass tanks provided with aerated recirculating
water with four replicate tanks for each treatment. Twenty post larvae with a mean
weight of 1.56 0.02g were hand-counted and stocked into each of the replicate tanks and fed
2, 4, 6 and 8 times a day, respectively. Final body weights were measured significantly lower
at 2 and 4 times/day (5.76 0.08 and 5.96±0.15g, respectively) than 6 and 8 times/day
(8.54±0.16 and 8.31 0.19g, respectively) treatments (P
https://isfj.areeo.ac.ir/article_109966_5f9f0f29bc1a968f2e91198ddc169ee0.pdf
2011-05-22
129
136
10.22092/isfj.2017.109966
Nutrition
Penaeus indicus
Feed Conversion Ratio
H.
Moradizadeh Fard
h.moradizadeh@yahoo.com
1
LEAD_AUTHOR
M.
Soudagar
sudagar_m@yahoo.cm
2
AUTHOR
S.
Gorgin
3
AUTHOR
A.
Pasandi
4
AUTHOR
ORIGINAL_ARTICLE
Determination of artificial propagation biotechnic of Barbus xanthopterus
This project was carried out in the year 2003 in Khuzestan province waters to determine
the best artificial propagation techniques for mass production of Barbus xanthopterus. The
fish is one of the most valuable and economic species in the area. The propagation was started
in late March and continued till late April while suitable temperature was 21-24.5 C. A
number of 23 female broodstock with mean weight and length 3.85±0.45kg and 64.95±21cm
respectively with a sex ratio of 2:1 male to female were used in the process. The amount of
hypophysis injection was 4mg/kg weight of fish and two injections with 10-12 h interval to
10:90 were undertaken. Spawning success was 87% in broodstock. Latency period was 15-
17h and the incubation duration was 59-60h in 23-25 C.The fish eggs has a low stickiness
and the count of dry and water-absorbed eggs were 480±32 and 287±25g, respectively. In the
twice-injected broodstock, the fertilization rate was 77.22±3.1%, the hatching rate was 81.2 ±1.89%
and the survival rate was 83.4±2%. Size of the dry and water-absorbed eggs was 1248±45 and
2110±125, respectively. Washing time with fertilization liquid was 10 min for removing
stickiness. In the end, 660 thousand larvae were produced and released to earthen ponds for
culture.
https://isfj.areeo.ac.ir/article_109967_6b66a154855f62a874d2ce779ac2dbed.pdf
2011-05-22
137
142
10.22092/isfj.2017.109967
Aquaculture
Propagation
Broodstock
SPAWNING
S.A.
Mortezavi Zadeh
saheb.mortazavi@gmail.com
1
LEAD_AUTHOR
J.
Moazedi
jmoazedi@yahoo.com
2
AUTHOR
M.
Yooneszadeh Feshalami
3
AUTHOR
E.
Jorfi
4
AUTHOR
ORIGINAL_ARTICLE
Morphology of swim bladder in false trevally (Lactarius lactarius)
The swim bladder status of false trevally (Lactarius Lactarius) was studied from November 2007 to October 2008 in coastal waters of the Oman Sea. A number of 702 swim bladders of false trevally specimens were collected randomly from catch composition of gillnets and bottom trawler. False trevally has an unusual swim bladder, as the first and the second spines of its anal fin are joined that creates a single bone, which is called anchorage bone, and passes from the middle of the swim bladder and 2 lobes of the gonad and connects to the 10th vertebra of the spinal column. Another ending of this bone is wide and reaches anal fin base. This bone is like an anchorage for swim bladder of false trevally and it functions as a center of gravity of the fish in anal fin base. It firms the exterior part of the fish body, so the fish swims through rough waters easily.
https://isfj.areeo.ac.ir/article_109968_6b79ab415a93fe7fda07a152f9d6f509.pdf
2011-05-22
143
146
10.22092/isfj.2017.109968
False Trevally
LACTARIUS LACTARIUS
Swim bladder
Oman Sea
M.
Doustdar
mastooreh_doustdar@yahoo.com
1
LEAD_AUTHOR
Gh.R.
Daryanabard
daryanabard@yahoo.com
2
AUTHOR
Gh.H.
Vosoughi
3
AUTHOR
R.
Rahmati
4
AUTHOR
ORIGINAL_ARTICLE
Spatial and temporal variability of macrobenthic communities in Chalus Shore of the Caspian Sea
The changes in density and biomass of macrobenthos were seasonally investigated from winter 2005 till fall 2006 in southern Caspian Sea. Samples were taken with a 225cm2 Van Veen grab from depths of 7 and 20 meters along two transects: Line 8 area (western part) and Radio Darya area (eastern part). Totally, 5 classes of macrobenthos were identified of which the most abundant populations were Polychaeta 68.7%, Oligochaeta 14.6%, Bivalvia 8.5%, Crustacea 8.2% and Gastropoda 0.08%. The maximum density (4251.8 1419 (ME SE) ind/m2) of macrobenthos was observed during summer in the depth of 20m of Radio Darya area, while the minimum density (725.9 64.5 (ME SE) ind/m2) was observed during spring in the depth of 7m in the same area. Between the two studied depths, the mean annual density in 7m depth (2266.7 350.3 (ME SE)ind/m2), was higher from 20m depth (1977.8 290.1 (ME SE)ind/m2). The maximum biomass (475 243.8 (ME SE)g/m2), of macrobenthos was observed during autumn in the depth of 20 meter of Radio Darya area, while the minimum biomass (0.3 0.1 (ME SE)g/m2), was observed during winter in the depth of 7m in the same area. Between the two studied depths, the mean annual biomass in 20m depth (184.8 24.6 (ME SE)g/m2), was higher from 7m depth (73.7 24.6 (ME SE)g/m2). Generally speaking, the eastern part of Chalus coast had more density and biomass compared with the western part. *Corresponding author
https://isfj.areeo.ac.ir/article_109969_b76e25b551ddaa91db0a87b91f0bf6bb.pdf
2011-05-22
147
152
10.22092/isfj.2017.109969
biomass
Macrobenthos
Ecology
Caspian Sea
M.
Tavoli
eysamtavoli@yahoo.com
1
LEAD_AUTHOR
M.
Islami
2
AUTHOR
S.M.
Mahdavi
3
AUTHOR