ORIGINAL_ARTICLE
Investigation of antibacterial activity of Virgularia juncea extracts from the Persian Gulf
Although Iran covers most of the northern coast from the Persian Gulf, marine organisms from this region have not been investigated very well. Herein, we aimed at a detailed analyses of antibacterial profiles against Escherichia coli ATCC, Bacillus subtilis spizizenii ATCC and Staphylococcus aureus ATCC of the marine sea pen Virgularia juncea collected from the Persian Gulf in Iran. A bioguided isolation approach was used to purify the active compounds based on the MIC and MBC parameters obtained for the extracts and fractions. The n-hexane, ethyl acetate and methanol extracts of V. juncea were tested in multiple in-house bioassays, and the ethyl acetate extract showed in vitro antibacterial activity against S. aureus (MIC value 7.5 µg/mL). This extract was fractionated by using open column chromatography. The isolated active fraction (F6d) which had high activity against the S. aureus strain at the lowest concentrations (MBC value 10 µg/mL) of other fractions, was purified using the HPLC method. The pure compound Cholest-5-en-3β-ol was isolated from the active fraction (F6d), and showed moderate activity against the S. aureus bacterial strain (MBC value 50 µg/mL). This compound was identified using the GC-MS technique. This is the first study that has dealt with bioactivity profiling of V. juncea, leading to isolation and identification of pure sea pen metabolite.
https://isfj.areeo.ac.ir/article_125139_f9ad501f5ffd5558b5302a994b5eca6e.pdf
2021-10-23
1
12
10.22092/isfj.2021.125139
Persian Gulf
Marine natural products
Sea pen
Virgularia juncea
Antibacterial
Reza
Mohsenian kochsari
1
AUTHOR
Melika
Nazemi
melikanazemi@yahoo.com
2
AUTHOR
Mehdi
Moridi farimani
3
AUTHOR
ORIGINAL_ARTICLE
Isolation of Aeromonas hydrophila effective phage from wastewater and using it in order to control the bacterial growth in minced meat of common carp (Cyprinus carpio)
In this study is to isolate and identify the molecular identity of effective phage on Aeromonas hydrophila in Urmia Urban Sewage System and control the experimental contamination in common minced carp fillet. For to isolate bacteriophage in the treated water, the existing bacteria were destroyed by pure chloroform in order to release the existing phage. Also the standard strains of Aeromonas hydrophila were used to evaluate the antibacterial assay of isolated bacteriophage (via CODE PTCC1310). Likewise, fish samples were prepared as fresh and minced. Then minced meat was contaminated with 108 CFU/g of Aeromonas hydrophila and four levels of effective bacteriophage, 108, 107, 106 and 105 PFU/g respectively, per one gram of meat were added and mixed. Meat samples containing negative and positive (2ppm of nisin) control and four levels of bacteriophage (108, 107, 106 and 105 PFU/g) were stored in refrigerator for a week. During the storage, sampling was done for bacterial count and bacteriophage titration. Results showed that effective phage on Aeromonas hydrophila isolated from Urmia sewage system could prevent the growth of bacteria. Also, findings showed that the levels of 10000 and 100000 PFU/g could significantly prevent the growth of Aeromonas hydrophila in common minced carp meat. It can be concluded that the phage effective on Aeromonas hydrophila in Urmia sewage system can suitably be used for biological control in minced meat of common carp (Cyprinus carpio).
https://isfj.areeo.ac.ir/article_125141_a99a8f6a5df4d3cf84d6aa4cfb9c3dc4.pdf
2021-10-23
13
25
10.22092/isfj.2021.125141
: Bacteriophage
Wastewater
common carp
minced meat
Aeromonas hydrophila
Samira
Sharafkhani
1
AUTHOR
Ahamad
gharehkahni
2
AUTHOR
ORIGINAL_ARTICLE
The study of distribution, abundance and biomass of Streblospio gynobranchiata in the sediments of southern coasts of Caspian Sea (Bandar Torkman and Babolsar)
The purpose of this study was to investigate the seasonal variation of distribution, abundance and biomass Streblospio gynobranchiata of substrate sediments on the Southern coasts of the Caspian Sea (Bandar Torkman and Babolsar) in 2018-2019. Sampling was done seasonally and 6 stations at depths of 5, 10 and 30 meters of the 2 transects. The results showed that the mean (±SE) of abundance and biomass were in the Babolsar transect (respectively) 207.06±51.40 ind.m-2 and 0.042±0.006 g.m-2 and seasonal study, the highest abundance and biomass in Babolsar transect with mean (respectively) 1297.47±284.95 ind.m-2 and 0.222±0.040 g.m-2. So there was a significant difference between the abundance and biomass in different seasons (p<0.05). Also showed that the mean (±SE) of abundance and biomass were in the Bandar Torkman transect (respectively) 206.51±53.07 ind.m-2 and 0.043±0.005 g.m-2. According to seasonal study, the highest abundance in Bandar Torkman transect with mean 942.27±180.07 ind.m-2 in summer and highest biomass 0.153 ± 0.03 g.m-2 recorded in spring, so there was a significant difference between the abundance and biomass in different seasons (p<0.05), which is due to their reproductive cycle. The total organic matter in different seasons was fluctuating, which could be due to the depth, substrate and ecological origin.
https://isfj.areeo.ac.ir/article_125142_22b0339017380e204196986be0dbe1b0.pdf
2021-10-23
27
37
10.22092/isfj.2021.125142
Abundance
biomass
Streblospio gynobranchiata
Bandar Torkman
Babolsar
Caspian Sea
Matin
Shakoori
matin.shakoori@yahoo.com
1
AUTHOR
Mohammadali
Afarii bandpey
2
AUTHOR
Hasan
Nasrollahzadeh saravi
hnsaravi@gmail.com
3
AUTHOR
Meysam
Erfani
4
AUTHOR
Iraj
Rajabi
5
AUTHOR
ORIGINAL_ARTICLE
Study of abundant and some reproduction traits of two species crabs Matuta victor (Fabricius, 1781) and Matuta planipes (Fabricius, 1798) in Gehrdo coast, Oman Sea
This study was done to investigate of abundance and some reproduction traits of Matuta victor and Matuta planipes on Gehrdo coast, the Oman Sea from December 2019 to November 2020. Overall, 310 crabs of M. victor and 297 crabs of M. planipes were collected. Sex ratio (M/F) was observed as 1.4:1 for M. victor and 1.7: 1 for M. planipes (p<0.05). Based on the results, the average carapace width and weight of male crabs were obtained more than female crabs for two studied species. In this study, the highest and lowest fecundity were 43423 eggs (carapace width of 26.7 mm and weight of 6.25 g) and 11635 eggs (carapace width of 28.55 mm and weight of 8.79 g) for M. victor and 45353 eggs (carapace width of 24.6 mm and weight of 6.35 g) and 14365 eggs (carapace width of 31.9 mm and weight of 10.29 g) for M. planipes. In two studied species, the result showed inversely relationship between egg number with carapace width and body weight. The results showed that there is an inverse relationship between temperature and gonadosomatic index for two studied species. The highest GSI was in February (10.71±6.44) and in October (5.41±2.13) for M. planipes and M. victor respectively. According to the results, abundance of M. planipes was more in autumn and winter while, abundance of M. victor was more in spring and summer.
https://isfj.areeo.ac.ir/article_125143_ed5b27ca97f86bf16fe87e33772b51bd.pdf
2021-10-23
39
51
10.22092/isfj.2021.125143
Sex ratio
fecundity
Crab
GSI
Mojtaba
Naderi
1
AUTHOR
Mina
Mahigir
2
AUTHOR
Fatemeh
Shahbazi
3
AUTHOR
ORIGINAL_ARTICLE
Vulnerability assessment of capture fishing major fish species based on demographic parameters in Persian Gulf and Oman Sea
The marine ecosystems are getting warmer and less oxygen and eventually becoming more acidic, there is also clear evidence that fish species are threatened by capture fishery on a regional and global scale. For this reason, the assessment of the inherent vulnerability of major species of catches is of great importance. There are several indicators of vulnerability classifications, and one of the most important is the biological and ecological characteristics of fish. This research tries to classify their vulnerability by examining the demographic parameters of major catch fish species in southern Iran and the Intergovernmental Panel on Climate Change (IPCC). In this study, according to the vulnerability index, which has values from 0 to 100, the following classification was performed. Low vulnerability fish (liza kluzengerii, Nemipterus japonicas) with values from 0 to 25, the medium vulnerability (Platycephalus indicus, Psettodes erumei) with values of 25 to 50, the high vulnerability (Pumpus argenteus, Tenualosa ilisha) 50 to 75, and very high vulnerability (Argyrosomus hololepidotus, Rhynchobatus djiddensis) 75 to 100. Undoubtedly, this type of classification can be useful for fishery managers and fishery managers and help them to better understand species and planning appropriately. In fact, we will build on this approach and have a better understanding of species variation and climate change, and will examine the risk of fish species being confronted with and their compatibility.
https://isfj.areeo.ac.ir/article_124874_11719ba8b18bfa8507f419db483243fa.pdf
2021-10-23
53
63
10.22092/isfj.2021.124874
Intrinsic vulnerability
Demographic parameters
Intergovernmental Panel on Climate Change (IPCC)
Seyed ahmadreza
Hashemi
1
AUTHOR
Seyed aminollah
Taghavi motlagh
2
AUTHOR
ORIGINAL_ARTICLE
Evaluation of antioxidant activity of hydrolyzed peptides from Portunus segnis shell Crab
True crabs are always part of the catch, especially trawling. Given the lack of direct consumption of crabs in the region, their shell waste can be used as a valuable source of protein to extract antioxidant compounds. In this study, samples of Portunus segnis crabs were collected by trawl nets with apertures of 40 mm from depths of 20 to 30 m from the west coast of Khuzestan province. The enzymes a-chymotrypsin, Trypsin, Pepsin, Papain, Neutrase and Alcalase were used to hydrolyze crab crust proteins. The antioxidant activity of the crab shell was investigated by removing the free radical activity of DPPH, reducing antioxidant power assay and measuring the ACE inhibitory activity of hydrolyzed proteins. The highest radical removal activity of diphenyl picrylhydrazyl (DPPH) was observed for proteins hydrolyzed with chymotrypsin and papain enzymes at 23.67±13.65 and 23.13 02.85, respectively. The lowest reduction power of hydrolyzing enzymes with chymotrypsin was 0.072 and the highest value for papain was 0.122. The level of inhibitory activity of crab shell hydrolyzates showed that the highest activity was related to Papain enzyme (16.20%) and the lowest was related to Alkalase enzyme (12.51%). According to the observations, it seems that crab shell proteins after enzymatic hydrolysis have moderate antioxidant activity, reducing power and inhibition of ACE, and probably after additional research of hydrolyzed proteins of crab exoskeleton as a suitable source of Antioxidant compounds can be used.
https://isfj.areeo.ac.ir/article_125169_16700abf953891decc465fe3dd1da541.pdf
2021-10-23
65
77
10.22092/isfj.2021.125169
Crab
DPPH
antioxidant peptides
enzyme hydrolysis
Pepsin
Zeynab
Ebadi
1
AUTHOR
Babak
Doostshenas
doustshenas@kmsu.ac.ir
2
AUTHOR
Nasrin
Sakhaii
nsakhaee@yahoo.com
3
AUTHOR
Kamal
ghanemi
4
AUTHOR
ORIGINAL_ARTICLE
Comparison of the effect of sodium hypochlorite and clay on the de-adhesion of fertilized egg of Siberian sturgeon (Acipenser baerii Brandt, 1869)
In the present study, sodium hypochlorite with a concentration 0.01% for 5 minutes was used as a new technique to de-adhesion of fertilized egg of Siberian sturgeon (Acipenser baerii) and its efficiency was compared with clay solution. The lowest amount of dead and fungal eggs (37.78±2.10%) and the highest fertilization (69.35±1.75%) and hatching rates (62.21±2.10%) were observed in eggs treated with sodium hypochlorite (p<0.05). Moreover, the highest weight (13.03±0.23 mg) and length (11.82±0.17 mm) of larvae were found in the de-adhesion treatment with sodium hypochlorite which had a significant difference with clay treatment (p<0.05). There was a significant difference in the incubation time between treatments (p<0.05), so that the de-adhesion treatment with clay had the highest incubation time (2093.75±21.62 degree-hour). The results of this study demonstrated that using sodium hypochlorite with a concentration of 0.01% is a simple, cheap and fast method for egg de-adhesion of Siberian sturgeon.
https://isfj.areeo.ac.ir/article_125189_ae3bf54266edde5dfae68ffbbde8b365.pdf
2021-10-23
79
93
10.22092/isfj.2021.125189
Acipenser baerii
Artificial reproduction
Egg de-adhesion
Sodium Hypochlorite
Bahram
Falahatkar
bfalahatkar@yahoo.com
1
AUTHOR
Hadiseh
Alizadeh
2
AUTHOR
Hamed
Abdollahpoor
3
AUTHOR
Naghmeh
Jaafari
4
AUTHOR
Mahdi
Rahmati
5
AUTHOR
ORIGINAL_ARTICLE
Study of the effect of oral troxerutin on resistance against Ichthyophthirius multifilis in goldfish (Carassius auratus)
Troxerutin (C33H42O19), known as vitamin P4, is a tri-hydroxyethylated derivative of natural bioflavonoid rutin. In the previous studies, the beneficial effects of troxerutin in enhancing the growth performance, physiological and immunological indices were proved. This study was designed to evaluate the effect of troxerutin on resistance against the exoparasite Ichthyophthirius multifilis in goldfish. Fish with mean weight 2.74±0.08 g were distributed in eight glass tanks and four experimental groups, including one control group and three treatment groups. During the feeding trail, fish were fed on diets supplemented with different levels of 0, 0.1, 0.3 and 0.5 g kg-1 troxerutin for 80 days. At the end of the trial, fish were exposed to theronts stage of I. multifilis at 4,000 theronts per fish. Results showed that different signs, including hyperemia and fin erosions, were prominent in the control group in comparison with the treated fish. Total numbers of parasite trophonts were also significantly decreased in the fish treated with 0.3 and 0.5 g kg-1 troxerutin compared to the control fish. These data indicated a positive effect of dietary troxerutin on disease resistance against exoparasite Ichthyophthirius multifilis in goldfish.
https://isfj.areeo.ac.ir/article_125190_91712716cf09df4821f39884ad0a5001.pdf
2021-10-23
95
105
10.22092/isfj.2021.125190
Troxerutin
Goldfish
Parasite
Ichthyophthirius multifilis
Shelaleh
Moosavi
1
AUTHOR
Najmeh
Sheikhzadeh
2
AUTHOR
Amin
Marandi
3
AUTHOR
ORIGINAL_ARTICLE
Evaluation of health trends of relocated corals in Chabahar Bay by Coral Watch and Line Intercept Transect method based on climatic parameters
In order to develop Shahid Beheshti port of Chabahar, about 20,000 coral colonies were moved from the port development plan to a new site located in the north of Shahid Kalantari port. A decade after the corals were transferred, it is essential to know their health in order to make future decisions in similar cases. In this study, while examining the trend of air temperature and water temperature of Chabahar Bay, by diving operations and using Line Intercept Transect and Coral Watch methods, the health status and coverage of corals was monitored monthly from 2011 to 2017. Climatic data required for this study were obtained from Chabahar Meteorological Station and the satellite database of ECMWF Center. The relationship between the variables was examined through Pearson correlation test and Man Kendall trend analysis and the capability of SPSS and Minitab software. According to the results, the health status and development of the mentioned corals has a decreasing trend to the extent that the percentage of live coral coverage has increased from 98% in 1390 to 71.5% in 1398. On the other hand, the color degree of the health index of branched corals with a score of 4.31 out of 6 was in the "good" condition and mass corals with a score of 3.59 points were in the "average" condition. Despite the negative correlation between temperature and health of corals and the existence of a significant upward trend in the parameter of air temperature, due to the insignificance of the upward trend of water temperature, it seems that other factors besides water temperature fluctuations in the downward trend of health of corals have been involved.
https://isfj.areeo.ac.ir/article_125208_d27ffebc21763d3295a6c638ffd62994.pdf
2021-10-23
107
117
10.22092/isfj.2021.125208
Coral
Coral Watch Method
Line Intercept Transect Method
Climate parameters Chabahar Bay
Mohammadreza
Alimoradi
1
AUTHOR
Mohammad
Baaghideh
2
AUTHOR
Alireza
Entezari
3
AUTHOR
Mohsen
Hamidiyanpoor
4
AUTHOR
Teymoor
Aminirad
5
AUTHOR
Alimehdi
Abkenar
6
AUTHOR
ORIGINAL_ARTICLE
Study on the dietary effects of Housefly larvae (Musca domestica) on fecundity and growth rate of Litopenaeus vannamei spawners
In order to evaluate the dietary effects of housefly larvae (Musca domestica) as an alternative to Perinereis nuntia, on fecundity rate and larval stages development of Pacific white leg shrimp Litopenaeus vannamei, 30 pairs of L. vannamei shrimp were stocked in 3 fiberglass tanks of 5 tones containing filtered seawater under identical conditions. The brooders were fed with 3 diets including conventional diets of hatchery (P. nuntia, squid, oysters and food pellets), along with 5% of housefly larvae as a supplement (Diet A), conventional diets of hatchery along with 10% of housefly larvae as an alternative to P. nuntia (Diet B) and conventional diets of hatchery as a control (Diet C). The experiment results showed that diet A presented significant differences in promoting fecundity rates of shrimp broodstocks with the other treatments (p<0.05). The number of hatching eggs (hatching) in the treatments did not show any significant difference (p>0.05). The larval development stages of hatched eggs produced with different treatments showed that a greater percentage of larvae from broodstocks fed with diet A have developed faster to the next stage than the other two treatments, which has shown a significant difference (p<0.05), especially in post-larvae stages. However, the use of housefly larvae diet as a supplement to conventional diets of hatchery can increase the fecundity rates of shrimp broodstocks and result in faster larval development; but as an alternative to the polychaete worm (P. nuntia) in conventional diets of the hatchery, have no effect on shrimp broodstock hatcheries.
https://isfj.areeo.ac.ir/article_125226_9acb333b3dcab124ac98c5a608589189.pdf
2021-10-23
119
129
10.22092/isfj.2021.125226
Post larvae
Vannamei shrimp
Polychaete worm
Housefly
Kiyoomars
Roohanighadikolaie
1
AUTHOR
Isa
Abdolalian
abdolalian_1969@yahoo.com
2
AUTHOR
Maryam
Moezzi
3
AUTHOR
Mohammadreza
Zahedi
zahedi2138@gmail.com
4
AUTHOR
Mohammad
Gorgig jaski
5
AUTHOR
Fariborz
Ehteshami
ehteshamei@yahoo.com
6
AUTHOR
Shahram
Dadgar
shdadgar@yahoo.com
7
AUTHOR
ORIGINAL_ARTICLE
Influence of different dietary Astaxanthin levels on hemolymph biochemical parameters, total carotenoid content and intestinal microbial flora of the oriental river prawn, Macrobrachium nipponense
In the present experiment, two hundred and twenty-five oriental river prawns (Macrobrachium nipponense) with mean weight of 1.40±0.05 gram were fed by five formulated diets containing different astaxanthin levels, zero (control), 50, 100, 150 and 200 milligrams per kilogram in glass aquarium tank for eight weeks. At the end of the culture period, after collecting hemolymph and also the tissues of intestinal tract, muscle and exoskeleton were sampled, hemolymph biochemical parameters, total carotenoid content and intestinal microbial flora in prawns were studied. Hemolymph biochemical parameters such as urea, urea nitrogen, glucose, creatinine and triglycerides were decreased with increasing of astaxanthin levels, while HDL and LDL increased significantly (p<0.05). However, uric acid, calcium, phosphorus and cholesterol were not affected by astaxanthin treatments (p>0.05). Treatments containing 150 and 200 mg/kg astaxanthin showed the lowest total bacterial count. Intestinal lactic acid bacteria also increased in the treatment of 150 mg/kg astaxanthin (p<0.05). Total carotenoid content of prawns was elevated by increasing dietary astaxanthin levels (p<0.05). Generally, the results of present study showed that increasing dietary astaxanthin levels improves hemolymph biochemical parameters, total carotenoid content and intestinal microbial flora of the oriental river prawn and adding 150 milligrams astaxanthin per kilogram of this pigment to the diet was suggested to improve the parameters that mentioned of this prawn.
https://isfj.areeo.ac.ir/article_125191_52cfbb0346ed8900b84d58a285c5094f.pdf
2021-10-23
131
147
10.22092/isfj.2021.125191
Astaxanthin
carotenoids
Dietary supplement
metabolism
Oriental river prawn
Mohammad
Etefaghdoost
1
AUTHOR
Hamid
Allafnoveyrian
navi@guilan.ac.ir
2
AUTHOR
Mirmasood
Sajadi
3
AUTHOR
Bahram
Falahatkar
bfalahatkar@yahoo.com
4
AUTHOR
ORIGINAL_ARTICLE
The effect of light intensity on lipid production in Chlamydomonas reinhardtii to produce biofuels
Global warming due to the excessive use of fossil fuels, environmental pollution, and rising greenhouse gases has led researchers to develop renewable energy sources such as the sun, wind, water, and biofuels. Microalgae, which are high in fatty acids, are suitable candidates for biofuel production.This study was designed to investigate the effect of different light intensities on the growth and accumulation of lipid in freshwater microalgae Chlamydomonas reinhardtii. For this purpose, microalgae were cultured at five light intensities of 50, 100, 200, 300, and 400 µmol m-2 s-1 for 20 days. The highest lipid content was (45.72±1.06%), and the lipid yield was (105.21±1.63 mg L−1 d−1), according to the growth curves in microalgae exposed to 300 µmol m-2 s-1. The amount of saturated fatty acids increased significantly with increasing light intensity, while monounsaturated and polyunsaturated fatty acids decreased. Among the fatty acids, methyl ester, palmitic acid, stearic acid, and alpha-linolenic acid showed the highest values at a light intensity of 300 µmol m-2 s-1. To evaluate the quality of biofuels, parameters such as saponification rate (SV), iodine value (IV), Cetane number (CN), degree of unsaturation (DU), long chain saturated factors (LCSF), and cold flow plugging properties (CFPP) was also estimated. The findings of this study showed that light intensity could improve the fat efficiency and biofuel properties obtained from the microalgae C. reinhardtii as a raw material in biofuel production.
https://isfj.areeo.ac.ir/article_125316_bdd4922706a9c523766c0bc22d8474ca.pdf
2021-10-23
149
160
10.22092/isfj.2021.125316
Biofuel
Growth
Lipid
Microalgae
Light intensity
Marzieh
Raaiiati
1
AUTHOR
Hooman
Rajabi eslami
2
AUTHOR
Mehdi
Shamsaii mehrjan
3
AUTHOR
ORIGINAL_ARTICLE
Molecular identification of Epixanthus frontalis (Brachyuran: Oziinae) from the rocky shores of Bushehr province using DNA barcode method
Epixanthus frontalis is the only known species of the genus Epixanthus from the Persian Gulf which include color polymorphic specimens. Considering that cryptic species are common among crustaceans, the aim of this study was to identify the cryptic species complexes the collected samples from the Persian Gulf. For this purpose, 4 colorful morphotypes of E. frontalis collected from rocky shores along Bushehr province. Then the first male gonopods was separated and photographed by scanning electron microscopy (SEM). For molecular studies samples of DNA extracted by using phenol-chloroform and mitochondrial cytochrome oxidase subunit gene segment (COI) was PCR and sequenced. The results showed that the nucleotide sequence of this gene fragment of the mitochondrial (COI) is not identical in all colorful morphotypes. These results reflected in the evolution of tree topology for all two analyses (maximum likelihood, bayesian). The study of apical ultrastructure segments of the first male species showed that it can be seen fundamental differences in the apical organs in some colorful morphotypes. Results of this study indicated that although the specimens assigned to single species morphologically, they may belong to different species based on their genotype. Further molecular studies with specific markers are needed for molecular identifications.
https://isfj.areeo.ac.ir/article_125320_9dd2eb0df954f1adca24d309ee0cb63b.pdf
2021-10-23
161
171
10.22092/isfj.2021.125320
COI
Ultrastructure
Gonopod
Persian Gulf
Farideh
Chenari
chenari_bio@yahoo.com
1
AUTHOR
Seyedmohammad bagher
nabavi
2
AUTHOR
Siyamak
Yousefi
3
AUTHOR
Mohammad ali
Salari
4
AUTHOR