Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
The determinant factors underlying habitat selectivity and preference for Black fish Capoeta capoeta gracilis (Keyserling 1891) in Siyahrud River (a tributary of Sefidrud River basin)
1
10
FA
H.
Asadi
asadi.shil@gmail.com
M.
Sattari
msattari@guilan.ac.ir
S.
Eagderi
soheil.eagderi@yahoo.com
10.22092/isfj.2014.103536
Understanding habitat requirements of aquatic organisms is key factor for sustainable exploitation and biodiversity conservation of riverine ecosystems. The habitat preference in fish has extremely important for the management of fish populations. The habitat requirements of Black fish (<em>Capoeta capoeta gracilis</em>) in Siyahrud River -tributaries of the Sefidrud River in Caspian basin- were studied in 13 stations along the river during October to November 2013. Hydrological characteristics of the river including water velocity, depth and width, slope, altitude, type of substrate materials, and plant coverage were recorded for each station. Ranges of habitat use and habitat selectivity with regard to the availability of habitat units for different variables were measured using HABSEL software. Despite significant correlation between the water velocity and depth, results from multiple regressions showed preference for the depth range of 17 to 38 cm. The results showed that behavioral preference in C<em>. capoeta gracilis</em> for the velocity was between 0.44 and 0.56 m/s. Also, specimens tended to be present in cobble stone substrate. Therefore, the anthropogenic activities over the Siyahrod River changing the river width, depth, substrate and the flow rate may affect the presence and distribution of <em>C. capoeta gracilis</em> in the mentioned river.
Black fish (C. capoeta gracilis),Environment variables,habitat,Siyahrud River
https://isfj.areeo.ac.ir/article_103536.html
https://isfj.areeo.ac.ir/article_103536_132e48ab093ddfb72be170bdb766db08.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota)
11
20
FA
S.
Bahroodi
s.bahroudi@gmail.com
M. A.
Nematollahi
M. R.
Aghasadeghi
M.
Nazemi
melikanazemi@yahoo.com
B.
Behroz
10.22092/isfj.2014.103537
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (<em>Holothuria leucospilota</em>) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
Sea cucumber,body wall,methanolic extract,diethyl etheric extract,Cytotoxic,anti- cancer
https://isfj.areeo.ac.ir/article_103537.html
https://isfj.areeo.ac.ir/article_103537_e5ffa7ef660afe73b9239e011cf92551.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Effect of herbicide Atrezine chronic toxicity on bioaccumulation process in fillet of Barbus grypus
21
30
FA
A.
Khabazian Zadeh
alikhabazian2011@gmail.com
A.
Dadolahi Sohrab
M.
Alishahi
S. H.
Khazaei
H. Mohammad
Asgari
10.22092/isfj.2014.103538
Atrazine is one of the most important and effective pollutant in aquatic ecosystems. The largest sugar cane farms of Middle East is located in Khouzestan Province, Iran in which large amounts of Atrezine are being used in farming. The aim of this study was to investigate acute toxicity (LC<sub>50</sub> 96 h) of atrazine on <em>barbus grypus</em> and the effects of chronic toxicity with sub-lethal concentration of atrazine on bioaccumulation of atrazine in fish fillet. LC<sub>50</sub> 96 h of atrazine on <em>barbus grypus </em>was measured according to the OECD standard method, 180<em> barbus</em> <em>grypus</em> were divided into 4 equal groups (in triplicates). Groups 1, 2 and 3 were exposed to 3.25 mg l-1 (5%), 6.5 mg l-1 (10%) and 13 mg l-1 (20%) of LC<sub>50</sub> 96 h concentrations, respectively. Group 4 exposed to toxin free water as a control group. Experimental exposures did last for 21 days, muscles samples of the large dorsal muscle were taken on days 0, 7, 14 and 21 of experiment. Bioaccumulation of Atrazine was measured in the muscle at days 0, 7, 14 and 21 in all groups. Results indicated that atrazine was toxic for <em>barbus</em> <em>grypus</em> and its toxicity increased not only with increase in atrazine but also with increase in the exposure time. The bioaccumulation of atrazine in fish muscles was increased significantly in groups 2 and 3 in all sampling periods and in groups 1 only in day 21.
The results of present study showed that placing <em>barbus</em> <em>grypus</em> in the presence of chronic and sub lethal concentrations of the herbicide Atrazine for three weeks may lead to accumulation of toxins in the fish fillets.
Atrazine,Chronic toxicity,Bioaccumulation,Barbus grypus
https://isfj.areeo.ac.ir/article_103538.html
https://isfj.areeo.ac.ir/article_103538_220f5bf59d94880454f886ecef00febf.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Effect of herbicide Atrezine chronic toxicity on bioaccumulation process in fillet of Barbus grypus
31
40
FA
A.
Dashtiannasab
adashtiannasab@gmail.com
M.
Mesbah
mehrmesbah@yahoo.com
R.
Peyghan
peyghan-r@scu.ac.ir
S.
Kakoolaki
bsh443@yahoo.com
10.22092/isfj.2014.103539
Atrazine is one of the most important and effective pollutant in aquatic ecosystems. The largest sugar cane farms of Middle East is located in Khouzestan Province, Iran in which large amounts of Atrezine are being used in farming. The aim of this study was to investigate acute toxicity (LC<sub>50</sub> 96 h) of atrazine on <em>barbus grypus</em> and the effects of chronic toxicity with sub-lethal concentration of atrazine on bioaccumulation of atrazine in fish fillet. LC<sub>50</sub> 96 h of atrazine on <em>barbus grypus </em>was measured according to the OECD standard method, 180<em> barbus</em> <em>grypus</em> were divided into 4 equal groups (in triplicates). Groups 1, 2 and 3 were exposed to 3.25 mg l-1 (5%), 6.5 mg l-1 (10%) and 13 mg l-1 (20%) of LC<sub>50</sub> 96 h concentrations, respectively. Group 4 exposed to toxin free water as a control group. Experimental exposures did last for 21 days, muscles samples of the large dorsal muscle were taken on days 0, 7, 14 and 21 of experiment. Bioaccumulation of Atrazine was measured in the muscle at days 0, 7, 14 and 21 in all groups. Results indicated that atrazine was toxic for <em>barbus</em> <em>grypus</em> and its toxicity increased not only with increase in atrazine but also with increase in the exposure time. The bioaccumulation of atrazine in fish muscles was increased significantly in groups 2 and 3 in all sampling periods and in groups 1 only in day 21.
The results of present study showed that placing <em>barbus</em> <em>grypus</em> in the presence of chronic and sub lethal concentrations of the herbicide Atrazine for three weeks may lead to accumulation of toxins in the fish fillets.
Atrazine,Sargassum angustifolium,Chronic toxicity,ethanolic extract,Bioaccumulation,Litopenaeus vannamei,Barbus grypus,Vibrio harveyi,Growth,survival
https://isfj.areeo.ac.ir/article_103539.html
https://isfj.areeo.ac.ir/article_103539_ce3ccddb1752ad82491a3cebc4317c04.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Effect of nicin z on some of spoilage chemical and bacterial properties in surimi of kilka (Clupeonella cultriventris caspia) stored in 4° c
41
56
FA
A.
Dehbandi
dr_dehbandy@yahoo.com
A. A.
Motallebi
motalebi@ifro.ir
V.
Razavilar
vr@ut.ac.ir
R.
Pourgholam
r_pourgholam@yahoo.com
10.22092/isfj.2014.103540
<span style="text-decoration: line-through;"></span>
The effect of antibacterial and antioxidant of nicin z in two forms (free and encapsulated) was investigated on <em>Total Viable Count</em>(<em>TVC</em>)<em> ,</em><em>Psychrotrophic Counts</em>(<em>PTC</em>) , <em>Peroxide Value</em>(PV)<em> ,</em> <em>Thiobarbituric acid</em><em> (</em><em>TBA</em><em>)</em> and<em> Total volatile nitrogen</em>(TVN) in zero, 3, 6, 9, 12, 15 days of storage.Two concentrations (700IU/gr ,1000IU/gr) of free and encapsulated nicin in liposome (by spray-dried method) were added as spray on surimi of kilka and one treatment was selected as a control. The results showed that change of chemical and bacterial parameters in treatment of encapsulated nicin was lower than free nicin and control treatments. Concentration of 1000IU/gr of nicin was better than results of other treatments. The shelf life of surimi of kilka in control, free and encapsulated nicin treatments for bacterial results were 9,12 , 15 days, respectively, and for<em>TBA </em>, TVN and PV were 6 , 15 and at least 15 days, respectively. The conclusion was that encapsulated nicin in liposome improved shelf life of surimi of kilka.
Free nicin,Encapsulated nicin,surim,Kilka
https://isfj.areeo.ac.ir/article_103540.html
https://isfj.areeo.ac.ir/article_103540_fe604426b6802678a02bd61b45db793d.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Introduce of metallic pollutant reducing bacteria (Fi , Ni) in
57
68
FA
M.
Ramin
ifro-mrifro@yahoo.com
M.
Doustdar
mastooreh_doustdar@yahoo.com
10.22092/isfj.2017.110172
In order to isolate bacterial strains resistant to iron and nickel, deposit samples were collected from five stations in Musa Bay and cultured on BHI agar medium containing 1000 ppm of iron and 50 ppm of nickel. Endurance threshold of strains isolated to different concentrations of iron (1000- 15000 ppm) and nickel (100- 25000 ppm) in BHI broth medium were evaluated, the most resistant strains were selected and MIC and MBC were determined. Results of the current study demonstrated that among 16 and 12 resistant strains to nickel and iron, two selected strains (Bacillus subtilis and Micrococcus sp. ) as the most resistant strains had MIC and MBC (29700 and 29800 ppm) and (26300 and 26500 ppm ), respectively, Ironâs MIC was not assessed. Results of atomic absorption analysis demonstrated that the highest amount of nickel reduction in a medium with concentration of 194 ppm and 71 ppm were 16.7% and 63% after 72 hours by selected strain of Bacillus subtilis respectively, and in a medium containing 158 ppm iron, after 50 hours 8.4% by selected strain 7, while dead biomass of strain 16 reduced nickel to the amount of 40.6% and it reduced 21% and 4% of iron in solutions with 46 ppm and 792 ppm of iron. Results of molecular analysis demonstrated that strain 16 was Bacillus subtilis. In addition, Bacillus subtilis being isolated in this study with ability of nickel and iron removal is an appropriate candidate to be used in aquatic environments.
Bacillus subtilis,Micrococcus sp,Fe,Ni,Musa Bay
https://isfj.areeo.ac.ir/article_110172.html
https://isfj.areeo.ac.ir/article_110172_b551d225cc67abbb89e29da1b8e37240.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Study of measuring Tilapia (Oreochromis niloticus) freshness using quality index method (QIM)
69
79
FA
G.
Zarh gashti
zarehgashti@yahoo.com
A.
Motalebi
motalebi@ifro.ir
Y.
Moradi
ymorady@yahoo.com
A.
Khanipour
aakhanipour@yahoo.com
N.
Mashaii
H.
Jalili
M.
Seifzade
F.
Rafipour
F.
Lagzaii
10.22092/isfj.2017.110173
Fish freshness by using QIM and according to scoring to all of parts variable (positive and negative) was by sensory method and finally become the parameters of quantitative and qualitative with statistical analysis is based, tables used in volatility score of 0 to 23, with a score of 0 to 3 respectively, for their excellent quality, 3 to 7good, from 7 to 10 and from 10 to above average evaluation is unacceptable.
In this study to measure the freshness of fish species Nile Tilapia (Oreochromis niloticus), black and red in 4 treatments, whole fish and empty stomach is considered and the objective of this study was to calculate the survival rate of fish in ice cover practically immediately after harvest to measure qualitative factors (sensory evaluation, chemical and microbiological).For this study 50 pieces of red and 50 pieces black Tilapia after weighed, washed and then selection 25 pieces for each treatment in unlit, ratio of 3 to 1 (ice - fish) was covered .The mean (± SD) change in temperature the fish flesh during study were between 0.2 ±0.07 a to 0.3 ±0.1a degrees Celsius, that need to raise the quality temperature is maintained at 0 degrees Celsius in unlit center.
Fish covered with ice keep at ambient temperature, and after measuring the change free nitrogen, pH and microbial tests, the results showed maximum shelf life of whole fish in ice cover 9 and for empty stomach fish was7 days .And also statistical analysis (Duncan test) of data between treatments (Whole and empty fish covered in ice ) was significant (P
Tilapia fish,Freshness,Sensory evaluation,Chemical changes
https://isfj.areeo.ac.ir/article_110173.html
https://isfj.areeo.ac.ir/article_110173_7db98319680d1c621765031f1a2862d8.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Utilization of poultry by product meal as an alternative protein source for Huso huso within growth period
81
96
FA
M. H.
Sayed Hassani
mirhamedhassani@yahoo.com
D.
Talebii Haghighi
M.
Hafeziehe
jhafezieh@yahoo.com
M. A.
Yazdani Sadati
H.M.
Pourali
H.
Yeganeh
10.22092/isfj.2014.103554
With regard to increasing the fish meal price, utilization of hygienic and analyzed poultry by product meal as an alternative protein source in diets for <em>Huso</em> <em>huso</em> within growth period were investigated. Six test diets isonitrogenous and isocaleric (contain 42% protein and 20 Mj kg<sup>-1 </sup>energy) were prepared. Fish meal was substituted by poultry by product at 20% (PBM<sub>20</sub>), 40% (PBM<sub>40</sub>), 60% (PBM<sub>60</sub>), 80% (PBM<sub>80</sub>) and 100% (PBM<sub>100</sub>) in base diet, respectively. A total of 180 <em>Huso huso</em> with average (±SD) weight of 107.89±5.9gr were stocked in 18 fiberglass tanks (Voloum:2000 lit,Temperature:20±2<sup>C</sup><sub>0</sub>) and fed satiation to 18 weeks. No significant differences were detected between final weight (FW),weight gain (WG) and specific growth rate of fish fed (PBM<sub>0</sub>), (PBM<sub>20</sub>), (PBM<sub>40</sub>), (PBM<sub>60</sub>) and (PBM<sub>80</sub>) respectively. The Highest protein belonged to fish fed PBM<sub>20 </sub>(%16.97±0.36)with significant difference with PBM<sub>40 (</sub>% 15.41 ± 0.67), respectively. Body lipid fish fed PBM80 and PBM100 (%6.63±0.016) (%7.49±0.17) were significantly lower than other treatments. The Synchronic hepatosomatic index increased with increasing poultry by product in diets and highest rate were found in fish fed PBM <sub>100</sub> , but except PBM<sub>20</sub>, visceral index in fish fed other treatments was same. The result showed that up to 80% fish meal could be replaced by poultry by product with no adverse effect on growth and feed conversion ratio for <em>Huso huso</em> within growth period.
Huso huso,Fish meal,Poultry by product,Growth rate,Body composition,Hepatosomat ic and Visceral index
https://isfj.areeo.ac.ir/article_103554.html
https://isfj.areeo.ac.ir/article_103554_a86792eb9ae8e7b7ef726dc092448087.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Effect of fluoxetine on food intake and feeding behavior of Goldfish Carassius auratus
97
108
FA
N.
Forsatkar
K.
Kukaram
M.A.
Nematolahi
malahi@ut.ac.ir
10.22092/isfj.2014.103555
Serotonin is one of the monoamines that play an important role in food intake in mammalian species. The increase in serotonergic activity may reduce appetite. Fluoxetine as an active ingredient of Prozac is increased in serotonin content by blocking reuptake of it. Due to the release of these substances into the ecosystem, the role of fluoxetine on food intake and feed behavior of goldfish, <em>Carassius</em> <em>auratus</em> was investigated. In experiment 1, fish with 21-49 g weight divided in four groups of control: with no injection; saline injected; and two groups with 1 µg g<sup>-1</sup> and 10 µg g<sup>-1 </sup>body weight fluoxetine. Animals were injected every other day for a total of 5 injections. Food intake calculated after each injection and fish reweighted at the end of the experiment to achieved weight changes. In experiment 2, 20 goldfish were selected in two groups of control and injected with 10 µg g<sup>-1 </sup>body weight fluoxetine to asses feed behavior test. Fish were injected every other day for a total of 5 injections and feed behavior was investigated after each injection. Food intake was significantly decreased after fluoxetine injections. In the Flu-10 group, low intake of food resulted in minimum weight gain among all treatments. Also, fluoxetine affected the feed behavior of goldfish and significantly was decreased in search and consumption of food. Results showed that this recently toxic environmental material can largely affect the food and weight parameters of goldfish.
Fluoxetine,Goldfish,IP injection,Food intake,FEEDING BEHAVIOR
https://isfj.areeo.ac.ir/article_103555.html
https://isfj.areeo.ac.ir/article_103555_c9b35a712836ffde04ea1337a1befb96.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
The effects of Zataria multiflora on inhibition of polyphenoloxidase and melanosis formation in shrimp
(Litopenaeus vannamei)
109
118
FA
E.
Nasiri
M.
Moosavi-Nasab
marzieh.moosavi-nasab@mail.mcgill.ca
S. S.
Shekarforoush
M. T.
Golmakani
10.22092/isfj.2014.103556
Shrimp melanosis (black spot) is an important surface discoloration caused by polyphenol oxidase (tyrosinase) enzyme, which oxidizes phenols and leads to insoluble black pigments, the melanins. Sulphiting agents are widely used as melanosis inhibitors; but, the hazards related to sulphated foods, such as allergic reactions and severe disorders in asthmatic patients have created a necessity to find the effective natural alternatives. The current study was accomplished to assay the in vitro antityrosinase effect of <em>Z. multiflora </em>EO as well as its capability to retard the melanosis formation in shrimp during iced storage. According to GC/MS results, carvacrol, thymol and p-cymene were the major components of <em>Z.multiflora</em> EO, representing 50.8, 14.4 and 10.6, respectively. DPPH radical scavenging activity of EO was 0.8±0.02 mg/ml and 63.2% of tyrosinase activity decreased when EO with a concentration of 0.25% was applied. Furthermore, it has been observed that immersing the shrimps in1% EO aqueous suspensionretarded themelanosis formation in shrimp during 10 days of iced storage. It can be concluded that <em>Z. multiflora </em>EO could be used as an effective natural processing aid to increase the shrimp shelf-life during iced storage. <br /> <br />
Melanosis,Pacific white shrimp,Tyrosinase,Zataria multiflora Boiss
https://isfj.areeo.ac.ir/article_103556.html
https://isfj.areeo.ac.ir/article_103556_f633a3865112cc9124172e5f13e92ca5.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
Some biological aspects of Mesopotamichthys sharpeyi in Shadegan Wetland, Iran
119
128
FA
S.A.
Hashemi
seyedahmad83@yahoo.com
R.
Ghorbani
F.
Kaymaram
farhadkaymaram@gmail.com
S.A.
Hossini
G.
Eskandari
A.
Hedayati
hedayati@gau.ac.ir
10.22092/isfj.2017.110174
Some biological aspects of Mesopotamichthys sharpeyi were studied in Shadegan Wetland, Iran. Monthly samples were collected from five stations: Doragh, Rogabe, Khorosy, Salmane and Atish. A total of 437 specimens were measured from April 2013 to March 2014. Mean (±SD) (Range) length values for the male and female were calculated as 252±28 (95-357), 249±38 (115-374)mm respectively and mean (±SD) (Range) weight values were 218±31 (18-366 ),239±39(15-651)g respectively. The length-weight relationships were calculated as W=0.000006L3.11 (R2=0.85) for male, W=0.000005L3.14 (R2= 0.86) for female. The spawning occurred from April to July, and length at maturity (LM), weight at maturity (WM) and production per biomass (P/B) were calculated for male and female as LM=208, 220 mm WM=97, 133 g and P/B= 0.53, 0.50 per year, respectively.
*Corresponding author
Mesopotamichthys sharpeyi,Shadegan wetland,Biological aspects,production per biomass
https://isfj.areeo.ac.ir/article_110174.html
https://isfj.areeo.ac.ir/article_110174_51cbc0d6b9df2bdfb49d241525486a0f.pdf
Agricultural Research,Education and Extension Organization
Iranian Scientific Fisheries Journal
1026-1354
2322-5998
23
3
2014
09
23
The effect of dry salting on the shelf life of big mouth saury (Scomberoides commersonnianus)
129
138
FA
Z.
Hadizadeh
zahrahadizadeh65@yahoo.com
N.
Mooraki
S.
Moini
dr_smoini@yahoo.com
10.22092/isfj.2017.110175
The present study investigated the effects of salting process on shelf life of big mouth saury (Scomberoides commersonnianus) filets. To determine the quality, chemical experiments including crude protein, peroxide value, crude lipid, ash-salt and moisture measurements were conducted at time intervals of 0, 15, 30, 90, 150 and 190 days. Moreover, fatty acid's profile was measured at time intervals of 0, 90 and 190 days. Mean (±SD) crude protein, crude lipid, ash-salt and moisture contents in fresh fish were 20.07±0.85, 6.32±0.42, 2.17±0.2and 71.44±0.19, respectively, reaching 30.5±1.19 8±0.12, 17.6±0.1 and 43.9±0.91, respectively, after salting and storing at the ambient temperatures for 190 days. The mean (±SD) peroxide was 0.05±0.01 meqO2 kg-1, and in fresh fish reached to 2.5±0.05 meqO2 kg-1 at the end of storage time as salted products. Variations in all of these factors were significant. According to the obtained results, the best time period for storing is 90 days. In the present study, 21 fatty acids were recognized. Total saturated, and unsaturated fatty acids in fresh samples (time 0) were 53.41 and 54.47%, respectively, and reached 56.99 and 39.92 at the end of storage time as the salted product. The result of the organoliptic showed that the new method of the dry salting give a better quality to the product in comparison with the traditional method of dry salting.
*Corresponding author
Scomberoides commersonnianus,Salting,Shelf life
https://isfj.areeo.ac.ir/article_110175.html
https://isfj.areeo.ac.ir/article_110175_780bcd12615ac26f15663826902ef8b5.pdf