Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Biotechnic of brood stocking, artificial propagation and some physiological indices in farmed Acipenser nudiventrisBiotechnic of brood stocking, artificial propagation and some physiological indices in farmed Acipenser nudiventris11211006610.22092/isfj.2017.110066FAM. BahmaniA. Yousefi JourdehiR. KazemiM. PourdehghaniA. HallajianS. DejhandianJ. JalilpourJournal Article20170619This study was conducted on farmed Acipenser nudiventris at the International Sturgeon
Research Institute from the winter of 2007 till summer 2010. A total of 11 farmed
A. nudiventris specimens (7 male and 4 female) were stocked in groups of 4 fish in eight
fiberglass tanks (4 tons capacity) on the basis of their sex. Males were fed with a diet with no
soybean but containing vitamins. Females were fed with a diet containing soybean and
vitamins with 38-40% protein, 13-15% fat, 19.5-20Mj/kg energy, respectively. GnRH was
used for artificial propagation of Acipenser nudiventris brood stocks twice for females (dose
10μg/kg with 80:20 ration) and during one occasion for males with a dose of 20 μg/kg. The
results of the study indicated that food composition (soybean and vitamins C and E) played a
significant and positive role in the reproduction system for females. No significant effect of
treatment was observed in Testosterone levels between mature (Mean ±SD: 60.6±8.07ng/ml)
and immature (24.42±4.87ng/ml) males, Likewise, no significant differences were found
in17α-Hydroxy Progestrone for female (Mean ±SD: 0.106±0.019ng/ml) and for immature
fishes (0.031±0.006ng/ml) significant differences was detected in the concentration of
Albomine (1.54±0.05mg/dl for females and 1.35±0.07mg/dl for immature females), but there
were no significant differences in other biochemical parameters including Glocose, Cortisol,
Chlosterole and Triglisride in males and females. There were no significant differences in
total weight and length, WBC, RBC, Hb, Hct, MCH, MCHC and MCV parameters in males
and females. This study is a step forward towards the development of artificial breeding and
rearing sturgeon fish in the country.This study was conducted on farmed Acipenser nudiventris at the International Sturgeon
Research Institute from the winter of 2007 till summer 2010. A total of 11 farmed
A. nudiventris specimens (7 male and 4 female) were stocked in groups of 4 fish in eight
fiberglass tanks (4 tons capacity) on the basis of their sex. Males were fed with a diet with no
soybean but containing vitamins. Females were fed with a diet containing soybean and
vitamins with 38-40% protein, 13-15% fat, 19.5-20Mj/kg energy, respectively. GnRH was
used for artificial propagation of Acipenser nudiventris brood stocks twice for females (dose
10μg/kg with 80:20 ration) and during one occasion for males with a dose of 20 μg/kg. The
results of the study indicated that food composition (soybean and vitamins C and E) played a
significant and positive role in the reproduction system for females. No significant effect of
treatment was observed in Testosterone levels between mature (Mean ±SD: 60.6±8.07ng/ml)
and immature (24.42±4.87ng/ml) males, Likewise, no significant differences were found
in17α-Hydroxy Progestrone for female (Mean ±SD: 0.106±0.019ng/ml) and for immature
fishes (0.031±0.006ng/ml) significant differences was detected in the concentration of
Albomine (1.54±0.05mg/dl for females and 1.35±0.07mg/dl for immature females), but there
were no significant differences in other biochemical parameters including Glocose, Cortisol,
Chlosterole and Triglisride in males and females. There were no significant differences in
total weight and length, WBC, RBC, Hb, Hct, MCH, MCHC and MCV parameters in males
and females. This study is a step forward towards the development of artificial breeding and
rearing sturgeon fish in the country.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Dietary administration of vitamin C and Lactobacillus rhamnosus in combination enhanced the growth and innate immune response of the rainbow trout, Oncorhynchus mykissDietary administration of vitamin C and Lactobacillus rhamnosus in combination enhanced the growth and innate immune response of the rainbow trout, Oncorhynchus mykiss132211006710.22092/isfj.2017.110067FAA. TookmehchiH. ShamsiS. MeshkiniR. DelshadA. Ghasemi MoghanjoeiJournal Article20170619The effects of dietary vitamin C and Lactobacillus rhamnosus on immunity and growth
performance were investigated in Oncorhynchus mykiss. For this purpose, 480 rainbow trout
(68±5g) were obtained from a local farm and acclimated to laboratory conditions for 10 days
and then divided into four groups in three replicates. During 30 days, juvenile rainbow trout
were fed diets supplemented with vitamin C (1g/kg) and L. rhamnosus (at 5 Ã 107 CFU/g) or a
control diet. Biometry was done at day -30 and blood samples were taken by caudal vein after
fish anesthesia with clove powder at day 0, 15, 30 and 45. Serum lysozyme activity,
alternative complement activity and total plasma immunoglobulin level were assayed as
innate immune response of rainbow trout. Results showed fish fed with vitamin c and L.
rhamnosus (group 3) statistically could improve fish growth performance. Also lysozyme
activity and alternative complement activity of serum significantly were higher in group three
than other groups, but total plasma level of immunoglobulin only was higher than all groups
at day 30. In conclusion dietary administration of vitamin C and L. rhamnosus in rainbow
trout diet could enhance the growth and innate immune response, but these properties need
further studies on the field applications.The effects of dietary vitamin C and Lactobacillus rhamnosus on immunity and growth
performance were investigated in Oncorhynchus mykiss. For this purpose, 480 rainbow trout
(68±5g) were obtained from a local farm and acclimated to laboratory conditions for 10 days
and then divided into four groups in three replicates. During 30 days, juvenile rainbow trout
were fed diets supplemented with vitamin C (1g/kg) and L. rhamnosus (at 5 Ã 107 CFU/g) or a
control diet. Biometry was done at day -30 and blood samples were taken by caudal vein after
fish anesthesia with clove powder at day 0, 15, 30 and 45. Serum lysozyme activity,
alternative complement activity and total plasma immunoglobulin level were assayed as
innate immune response of rainbow trout. Results showed fish fed with vitamin c and L.
rhamnosus (group 3) statistically could improve fish growth performance. Also lysozyme
activity and alternative complement activity of serum significantly were higher in group three
than other groups, but total plasma level of immunoglobulin only was higher than all groups
at day 30. In conclusion dietary administration of vitamin C and L. rhamnosus in rainbow
trout diet could enhance the growth and innate immune response, but these properties need
further studies on the field applications.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Determining level of PCBs in skin and muscle tissue of Cyprinus carpio and Esox lucius in Anzali Wetland (Abkenar)Determining level of PCBs in skin and muscle tissue of Cyprinus carpio and Esox lucius in Anzali Wetland (Abkenar)233011006810.22092/isfj.2017.110068FAB. TeymouriS.M.B. NabaviSh. SafaeyanS.H. KhatamiJournal Article20170619The aim of this research is to determine the levels of polycholoro biphenil (PCBs) in skin
and muscle tissues of common carp (Cyprinus carpio) and common pike (Esox lucius) fishes
in the west part of Anzali wetland (Abkenar) in the two seasons, autumn 2009 and winter
2010. In each seasons, nine fishes from each species were selected randomly. The samples,
transferred in cool condition and the muscles and skins of them were removed. All of the
preparations organs clean up and PCBS analysis were carried with GC-ECD (Gas
chromatography). Mean concentrations of PCBS were 24.36пg/g dry.w in skin 14.36пg/g
dry.w in muscle tissue for E. lucius and 21.84пg/g dry.w in skin and 20.86пg/g dry.w in
muscle tissue of C. carpio. In the autumn, the values was less than FDI standard and food
standard Australia/AZ MRL and skin tissue of E. lucius and muscle tissue of C. carpio was
over of US EPA standard values. Mean concentration of PCBS were 29.43пg/g dry.w in skin
and 10.10ng/g dry.w in muscle tissue for E. lucius and 33.06пg/g dry.w in skin and 11.00пg/g
dry.w in muscle tissue for C. carpio. In the winter, the value was less than FDI standard and
food standard Australia /AZ MRL and skin tissue of E. lucius and skin tissue of C. carpio was
over of US EPA standard values. Mean concentration of PCBs in water in the autumn
(0.0042ppb) was less than recommended water quality Criteria E.P.A and in the winter
(0.1938ppb) was over recommended water quality Criteria E.P.A. Mean concentration of
PCBs in sediment in the autumn (4.89ng/g dry.w) and winter (4.48ng/g dry.w) was less than
Interim Canadian Sediment Quality Guidelines. There were significant differences between E.
lucius mussels and C. carpio skin tissue in two seasons (autumn and winter) in water and
ingredient suspension. But in sediments, no significant difference was detected in autumn and
winter.
*Corresponding authorThe aim of this research is to determine the levels of polycholoro biphenil (PCBs) in skin
and muscle tissues of common carp (Cyprinus carpio) and common pike (Esox lucius) fishes
in the west part of Anzali wetland (Abkenar) in the two seasons, autumn 2009 and winter
2010. In each seasons, nine fishes from each species were selected randomly. The samples,
transferred in cool condition and the muscles and skins of them were removed. All of the
preparations organs clean up and PCBS analysis were carried with GC-ECD (Gas
chromatography). Mean concentrations of PCBS were 24.36пg/g dry.w in skin 14.36пg/g
dry.w in muscle tissue for E. lucius and 21.84пg/g dry.w in skin and 20.86пg/g dry.w in
muscle tissue of C. carpio. In the autumn, the values was less than FDI standard and food
standard Australia/AZ MRL and skin tissue of E. lucius and muscle tissue of C. carpio was
over of US EPA standard values. Mean concentration of PCBS were 29.43пg/g dry.w in skin
and 10.10ng/g dry.w in muscle tissue for E. lucius and 33.06пg/g dry.w in skin and 11.00пg/g
dry.w in muscle tissue for C. carpio. In the winter, the value was less than FDI standard and
food standard Australia /AZ MRL and skin tissue of E. lucius and skin tissue of C. carpio was
over of US EPA standard values. Mean concentration of PCBs in water in the autumn
(0.0042ppb) was less than recommended water quality Criteria E.P.A and in the winter
(0.1938ppb) was over recommended water quality Criteria E.P.A. Mean concentration of
PCBs in sediment in the autumn (4.89ng/g dry.w) and winter (4.48ng/g dry.w) was less than
Interim Canadian Sediment Quality Guidelines. There were significant differences between E.
lucius mussels and C. carpio skin tissue in two seasons (autumn and winter) in water and
ingredient suspension. But in sediments, no significant difference was detected in autumn and
winter.
*Corresponding authorAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Effect of freezing on the chemical quality changes and fatty acid composition of cultured shrimp muscle, Litopenaus vannameiEffect of freezing on the chemical quality changes and fatty acid composition of cultured shrimp muscle, Litopenaus vannamei314411006910.22092/isfj.2017.110069FAM. Javaheri BaboliR. ChoiA. Askary SaryL. RoomianiJournal Article20170619Effect of freezing on the chemical quality and fatty acid composition of cultured shrimp
muscle, Litopenaus vannamei were investigated by measuring moisture content, ash, total
protein content, total lipid content, fatty acid composition, and Thio barbituric Acid (TBA)
during 6 month keeping in frozen storage at 18°C. According to the results, moisture content
(75.93% to 73.10%), ash (1.5% to 2.07%), total protein content (25.3% to 20.87%) and total
lipid content (0.83% to 0.23%) changed during six month of frozen storage. PUFA (45.21%)
content was higher than the SFA (30.08%) and MUFA (19.32%) content. The poly chain
unsaturated fatty acids, saturated fatty acids and mono chain unsaturated fatty acids in shrimp
muscle were C18:2n6 (15.32%), C20:5n3 (9.68%), C22:6n3 (8.48%), C16:0(15.18%), C18:0
(13.04%) and C18:1n9 (15.32%), respectively. The thio barbituric acid values (TBA) ranged
from 0.0065 to 0.35 mg malonaldehyde/kg during freezing storage. The lipid stability of
shrimp muscle, were in acceptable limit during frozen storage for up to 6 month.
*Corresponding authorEffect of freezing on the chemical quality and fatty acid composition of cultured shrimp
muscle, Litopenaus vannamei were investigated by measuring moisture content, ash, total
protein content, total lipid content, fatty acid composition, and Thio barbituric Acid (TBA)
during 6 month keeping in frozen storage at 18°C. According to the results, moisture content
(75.93% to 73.10%), ash (1.5% to 2.07%), total protein content (25.3% to 20.87%) and total
lipid content (0.83% to 0.23%) changed during six month of frozen storage. PUFA (45.21%)
content was higher than the SFA (30.08%) and MUFA (19.32%) content. The poly chain
unsaturated fatty acids, saturated fatty acids and mono chain unsaturated fatty acids in shrimp
muscle were C18:2n6 (15.32%), C20:5n3 (9.68%), C22:6n3 (8.48%), C16:0(15.18%), C18:0
(13.04%) and C18:1n9 (15.32%), respectively. The thio barbituric acid values (TBA) ranged
from 0.0065 to 0.35 mg malonaldehyde/kg during freezing storage. The lipid stability of
shrimp muscle, were in acceptable limit during frozen storage for up to 6 month.
*Corresponding authorAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121A survey of salinity tolerance of Persian sturgeon larvae (Acipencer persicus) fed with enriched live food containing HUFA and vitamin CA survey of salinity tolerance of Persian sturgeon larvae (Acipencer persicus) fed with enriched live food containing HUFA and vitamin C455411007010.22092/isfj.2017.110070FAM. HafeziehH. HosseinpourJournal Article20170619Salinity tolerance in Persian sturgeon larvae (Acipenser persicus) fed with enriched
Artemia urmiana nauplii were investigated when exposed to different salinity. For this
purpose, cyst of Artemia urmiana hatched and enriched with a commercial emulsion,
ICES30/4 supplemented with three levels of vitamin C (10, 20 and 30%) each with 3
replications according to standard condition and fed to Persian sturgeon larvae during 20
experimental days. At the end of this period, total length, weight, lipid, unsaturated fatty acids
contents and vitamin C in fish larvae were measured. Fish larvae were exposed to 6, 12 and
18ppt salinity, and salinity tolerance were surveyed after 120h. The results showed that
survival percentage as salinity tolerance indicator increased compared to control group that
are larvae fed un-enriched Artemia nauplii. ICES40/3 with 20% vitamin C led to the highest
salinity tolerance at 12ppt in Persian sturgeon larvae (99±1) but in 18ppt all larvae were died.
The increase in salinity tolerance is likely due to omega 3 and 6 increase in fish larvae fed
with Artemia enriched with ICES30/4.Salinity tolerance in Persian sturgeon larvae (Acipenser persicus) fed with enriched
Artemia urmiana nauplii were investigated when exposed to different salinity. For this
purpose, cyst of Artemia urmiana hatched and enriched with a commercial emulsion,
ICES30/4 supplemented with three levels of vitamin C (10, 20 and 30%) each with 3
replications according to standard condition and fed to Persian sturgeon larvae during 20
experimental days. At the end of this period, total length, weight, lipid, unsaturated fatty acids
contents and vitamin C in fish larvae were measured. Fish larvae were exposed to 6, 12 and
18ppt salinity, and salinity tolerance were surveyed after 120h. The results showed that
survival percentage as salinity tolerance indicator increased compared to control group that
are larvae fed un-enriched Artemia nauplii. ICES40/3 with 20% vitamin C led to the highest
salinity tolerance at 12ppt in Persian sturgeon larvae (99±1) but in 18ppt all larvae were died.
The increase in salinity tolerance is likely due to omega 3 and 6 increase in fish larvae fed
with Artemia enriched with ICES30/4.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Comparison of produced fish protein hydrolysete from viscera and head of Silver carp (Hypophthalmichthys molitrix) using Alcalase enzyme and internal tissue enzymesComparison of produced fish protein hydrolysete from viscera and head of Silver carp (Hypophthalmichthys molitrix) using Alcalase enzyme and internal tissue enzymes556211007110.22092/isfj.2017.110071FASh. HosseiniA. GhoroghiH.R. JamalzadehR. SafariSh. HosseiniJournal Article20170619In the present study, hydrolysed protein of viscera and head of Silver carp
(Hypophthalmichthys molitrix) was compared using Alcalase enzyme and internal tissue
enzymes at 2 and 4 hours. The result indicated that product by Alcalase (Treatment 1) had
significantly higher protein and rate of hydrolysates than that produced by internal tissue
enzymes (Treatment 2). So, the highest mean (±SD) protein (68.10±1.33) was related to
treatment 1-head (with Alcalase enzyme) after 4 hours and the highest rate of hydrolysates
(29.36±1.35) was related to treatment 1-head (with Alcalase enzyme) after 4 hours. The result
indicated that rate of hydrolysates raised as time of hydrolysates increased. However the
intensity and rate of hydrolysates is reduced. The highest rate of hydrolysates occurred at 120
minutes in the first. This mode was similar for two treatments. The result can be considered as
the Alcalase was preferred to internal enzyme.In the present study, hydrolysed protein of viscera and head of Silver carp
(Hypophthalmichthys molitrix) was compared using Alcalase enzyme and internal tissue
enzymes at 2 and 4 hours. The result indicated that product by Alcalase (Treatment 1) had
significantly higher protein and rate of hydrolysates than that produced by internal tissue
enzymes (Treatment 2). So, the highest mean (±SD) protein (68.10±1.33) was related to
treatment 1-head (with Alcalase enzyme) after 4 hours and the highest rate of hydrolysates
(29.36±1.35) was related to treatment 1-head (with Alcalase enzyme) after 4 hours. The result
indicated that rate of hydrolysates raised as time of hydrolysates increased. However the
intensity and rate of hydrolysates is reduced. The highest rate of hydrolysates occurred at 120
minutes in the first. This mode was similar for two treatments. The result can be considered as
the Alcalase was preferred to internal enzyme.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Morphological study of epithelial tissue of tail spine in Cow-tail stingray (Pastinachus sephen) brown eagle ray (Aetobatus flagellum) and butterfly ray (Gymnura poecilura) in the Persian Gulf and Oman SeaMorphological study of epithelial tissue of tail spine in Cow-tail stingray (Pastinachus sephen) brown eagle ray (Aetobatus flagellum) and butterfly ray (Gymnura poecilura) in the Persian Gulf and Oman Sea637211007210.22092/isfj.2017.110072FAH. DehghaniM.M. SajjadiP. PartoH. RajaeianJ. JalaeiJournal Article20170619Ray fishes have spines on their whip-like tails. In this study, histological survey was
undertaken to explore venom secretory cells in spines of the 3 species of ray fish in the
Persian Gulf and Oman Sea. Separated spines were preserved in formalin to transfer them to
the laboratory, and spines were dip in EDTA 4%, for decalcification and in the end part,
histological study were done. Results showed that venom secretory cells occurred in cow-tail
stingray (P. sephen) but no venom secretory cell was found in brown eagle ray (A. flagellum)
and butterfly ray (G. poecilura) species. This suggest that layers covering spine is similar to
common covering structure of fishe body.Ray fishes have spines on their whip-like tails. In this study, histological survey was
undertaken to explore venom secretory cells in spines of the 3 species of ray fish in the
Persian Gulf and Oman Sea. Separated spines were preserved in formalin to transfer them to
the laboratory, and spines were dip in EDTA 4%, for decalcification and in the end part,
histological study were done. Results showed that venom secretory cells occurred in cow-tail
stingray (P. sephen) but no venom secretory cell was found in brown eagle ray (A. flagellum)
and butterfly ray (G. poecilura) species. This suggest that layers covering spine is similar to
common covering structure of fishe body.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121The effects of glucan on hematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum fryThe effects of glucan on hematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum fry738411007310.22092/isfj.2017.110073FAR. RufchaieS.H. HoseinifarM. Sayad BoraniH. Maghsodie KohanA.A. ZaminiM. FaeedJournal Article20170619The purpose of this study was to determine the effect of dietary glucan on some
haematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum.
In the present study, various levels of ingredient so called Hoplit (0, 0.5, 1.0, 1.5, and 2.0%)
containing glucan was added to a basal formulated diet. Twenty and five kutum fry with a
mean (±SD) weight of 1.15±0.06g were stocked in each experimental tank (100 l capacity)
filled with 80 liter of water. Fish were fed on experimental diet for 56 days and biometry was
performed every 15 days. At the end of the trial blood samples were collected for
measurement of haematological parameters including: Red and white blood cells count,
differential count of white blood cells, hematocrit and hemoglobin, and innate immune factors
(Immunoglobulin and Lysozyme). Fries in 1.5% treatment had highest serum immunoglobins
(Ig) and eosinophil, although when compared with control but with no significant differences.
Highest MCHC, hematocrit and hemoglobin were observed in the 0.5 and 1% treatments and
the highest MCV and red blood cell count were in 0.5 percent treatment. The highest white
blood cells count and neutrophils was observed in 2% treatment. The highest and lowest
levels of lysozyme activity were observed in 1% and control treatments, respectively.
Evaluation of the total bacteria and LAB counts revealed significant increase in 0.5%
treatment. According to these results administration of dietary glucan can be considered for
stimulation of innate immune response of white fish fry.The purpose of this study was to determine the effect of dietary glucan on some
haematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum.
In the present study, various levels of ingredient so called Hoplit (0, 0.5, 1.0, 1.5, and 2.0%)
containing glucan was added to a basal formulated diet. Twenty and five kutum fry with a
mean (±SD) weight of 1.15±0.06g were stocked in each experimental tank (100 l capacity)
filled with 80 liter of water. Fish were fed on experimental diet for 56 days and biometry was
performed every 15 days. At the end of the trial blood samples were collected for
measurement of haematological parameters including: Red and white blood cells count,
differential count of white blood cells, hematocrit and hemoglobin, and innate immune factors
(Immunoglobulin and Lysozyme). Fries in 1.5% treatment had highest serum immunoglobins
(Ig) and eosinophil, although when compared with control but with no significant differences.
Highest MCHC, hematocrit and hemoglobin were observed in the 0.5 and 1% treatments and
the highest MCV and red blood cell count were in 0.5 percent treatment. The highest white
blood cells count and neutrophils was observed in 2% treatment. The highest and lowest
levels of lysozyme activity were observed in 1% and control treatments, respectively.
Evaluation of the total bacteria and LAB counts revealed significant increase in 0.5%
treatment. According to these results administration of dietary glucan can be considered for
stimulation of innate immune response of white fish fry.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Population dynamics of dominant shrimps in northeast of Qeshm IslandPopulation dynamics of dominant shrimps in northeast of Qeshm Island859811007410.22092/isfj.2017.110074FAM. SafaieJournal Article20170619The Chaoo Sharghi is located nearby mangrove forest in northwest of Qeshm Island, in the
northern Persian Gulf. Given standing in migration of juvenile shrimps, this area is one of
important regions in Hormozgan Province. This study aimed to determine population
dynamics of major shrimp stocks by swept area method in this area. Sampling was carried out
monthly since March 2009 to June 2010. The average of carapace length frequency of
Banana, Jinga, Kiddy and Peregrine shrimps showed that upswing from January to April.
However, shrimps were predominantly juvenile. The annual growth coefficient (k) of female
and male was 1.5 and 1.1 for F. merguiensis, 1.3 and 1.2 for M. affinis, 1.1 and 1 for M.
stebbingi and 1.2 and 1.1 for P. stylifera, respectively. The asymptotic carapace length (CLâ)
of female and male was 44mm and 31mm for P. merguiensis, 36mm and 31mm for M.
affinis, 28mm and 18.5mm for M. stebbingi and 27.5mm and 18.5mm for P. stylifera,
respectively. The mortality parameters of male and female shrimps were different in range
including 2.36 â 4.54 for total mortality, about, 2.12â1.83 for natural mortality and 0.24â2.50
for fishing mortality. Total mortality rate for M. affinis and M. stebbingi showed that this rate
for males was higher than females. Conversely, total mortality rate for F. merguiensis and P.
stylifera was higher for females than males. Exploitation rate for all species was higher in
females than that for males. In this study, the highest estimated biomass was found in Kiddy
and Jinga shrimps and the lowest biomass was found in Indian white shrimp. The peak of
biomass and CPUE was obtained during July-August.The Chaoo Sharghi is located nearby mangrove forest in northwest of Qeshm Island, in the
northern Persian Gulf. Given standing in migration of juvenile shrimps, this area is one of
important regions in Hormozgan Province. This study aimed to determine population
dynamics of major shrimp stocks by swept area method in this area. Sampling was carried out
monthly since March 2009 to June 2010. The average of carapace length frequency of
Banana, Jinga, Kiddy and Peregrine shrimps showed that upswing from January to April.
However, shrimps were predominantly juvenile. The annual growth coefficient (k) of female
and male was 1.5 and 1.1 for F. merguiensis, 1.3 and 1.2 for M. affinis, 1.1 and 1 for M.
stebbingi and 1.2 and 1.1 for P. stylifera, respectively. The asymptotic carapace length (CLâ)
of female and male was 44mm and 31mm for P. merguiensis, 36mm and 31mm for M.
affinis, 28mm and 18.5mm for M. stebbingi and 27.5mm and 18.5mm for P. stylifera,
respectively. The mortality parameters of male and female shrimps were different in range
including 2.36 â 4.54 for total mortality, about, 2.12â1.83 for natural mortality and 0.24â2.50
for fishing mortality. Total mortality rate for M. affinis and M. stebbingi showed that this rate
for males was higher than females. Conversely, total mortality rate for F. merguiensis and P.
stylifera was higher for females than males. Exploitation rate for all species was higher in
females than that for males. In this study, the highest estimated biomass was found in Kiddy
and Jinga shrimps and the lowest biomass was found in Indian white shrimp. The peak of
biomass and CPUE was obtained during July-August.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121The comparison of heavy metals (Hg, Cd, Pb) in the muscle of Otolithes ruber in Abadan and Bandar Abbas Ports, the Persian GulfThe comparison of heavy metals (Hg, Cd, Pb) in the muscle of Otolithes ruber in Abadan and Bandar Abbas Ports, the Persian Gulf9910611007510.22092/isfj.2017.110075FAA. Askary SaryM. Javahery BaboliS. MahjobM. VelayatzadehJournal Article20170619A comparative study was conducted on concentration of heavy metals including: Hg, Cd
and Pb in the muscle of Otolithes ruber in Abadan and Bandar Abbas Ports in the Persian
Gulf. In this study, a total of 42 specimens of Otolithes ruber were collected from coastal
waters of Abadan and Bandar Abbas Ports in 2010. Heavy metals were extracted from the
muscle tissues using wet digestion method and concentration of metals were measured by
Atomic Absorption Spectrophotometer. The results showed that mean (±SD) accumulation of
Hg, Cd and Pb in the muscles of Otolithes ruber in Bandar Abbas were 0.058±0.001,
0.279±0.056 and 0.668±0.077mgKg-1 wet weight, and in Abadan were 0.056±0.007,
0.25±0.038 and 0.638±0.087mgKg-1 wet weight respectively. No significant differences were
found in concentrations of Hg, Cd and Pb in the muscle of Otolithes ruber between Abadan
and Bandar Abbas ports. Accumulation of these metals were higher in the muscle of Otolithes
ruber than that Bandar Abbas Port. Concentration of Hg in the muscle of Otolithes ruber was
lower than acceptable limit suggested by WHO, MAFF, NHMRC. However concentrations of
Pb and Cd were higher.
*Corresponding authorA comparative study was conducted on concentration of heavy metals including: Hg, Cd
and Pb in the muscle of Otolithes ruber in Abadan and Bandar Abbas Ports in the Persian
Gulf. In this study, a total of 42 specimens of Otolithes ruber were collected from coastal
waters of Abadan and Bandar Abbas Ports in 2010. Heavy metals were extracted from the
muscle tissues using wet digestion method and concentration of metals were measured by
Atomic Absorption Spectrophotometer. The results showed that mean (±SD) accumulation of
Hg, Cd and Pb in the muscles of Otolithes ruber in Bandar Abbas were 0.058±0.001,
0.279±0.056 and 0.668±0.077mgKg-1 wet weight, and in Abadan were 0.056±0.007,
0.25±0.038 and 0.638±0.087mgKg-1 wet weight respectively. No significant differences were
found in concentrations of Hg, Cd and Pb in the muscle of Otolithes ruber between Abadan
and Bandar Abbas ports. Accumulation of these metals were higher in the muscle of Otolithes
ruber than that Bandar Abbas Port. Concentration of Hg in the muscle of Otolithes ruber was
lower than acceptable limit suggested by WHO, MAFF, NHMRC. However concentrations of
Pb and Cd were higher.
*Corresponding authorAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Age structure and growth rate of (Squalius cephalus Linnaeus, 1758) in Tuji tributary from Talar River, Mazandaran ProvinceAge structure and growth rate of (Squalius cephalus Linnaeus, 1758) in Tuji tributary from Talar River, Mazandaran Province10711811007610.22092/isfj.2017.110076FAM.H. Gorjian ArabiS.A. HosseiniM. RoohiR. PatimarS. VatandustE. AlijanpourJournal Article20170619A structure and growth pattern of Squalius cephalus (Linnaeus, 1758) in Tuji tributary of
Talar River, Mazandaran Province, was studied during the period from August 2008 till
August 2009. In total, 298 fish were caught by electrofishing and fixed in 10% formalin. The
weight and length of fish were measured and the age was determinated by scallometric
method. The age data showed that fishes were between 0+ to 4+ age groups. Pauli`s applied
growth pattern showed a positive alometric for male and female and total fish and a negative
isometric for unmature fishes. The average instantaneous growth rate of European Chub
showed that the age groups 2+ and 3+ with 1.1 had the fastest growth compare to 3+ and 4+ age
groups with 0.89. Condition factor of specimens showed that the highest one with 1.58 (r2
=
0.983) belong to females and the lowest with 1.17 (r2
= 0.901) belong to unmature fishes. The
total sex ratio (123 males to 115 females) showed no significant differences between them.A structure and growth pattern of Squalius cephalus (Linnaeus, 1758) in Tuji tributary of
Talar River, Mazandaran Province, was studied during the period from August 2008 till
August 2009. In total, 298 fish were caught by electrofishing and fixed in 10% formalin. The
weight and length of fish were measured and the age was determinated by scallometric
method. The age data showed that fishes were between 0+ to 4+ age groups. Pauli`s applied
growth pattern showed a positive alometric for male and female and total fish and a negative
isometric for unmature fishes. The average instantaneous growth rate of European Chub
showed that the age groups 2+ and 3+ with 1.1 had the fastest growth compare to 3+ and 4+ age
groups with 0.89. Condition factor of specimens showed that the highest one with 1.58 (r2
=
0.983) belong to females and the lowest with 1.17 (r2
= 0.901) belong to unmature fishes. The
total sex ratio (123 males to 115 females) showed no significant differences between them.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Changes of hormons (T3, T4 and cortisol) and ions (Na+, Cl-, K+) during smoltification in Salmo trutta caspius Kessler 1877Changes of hormons (T3, T4 and cortisol) and ions (Na+, Cl-, K+) during smoltification in Salmo trutta caspius Kessler 187711912811007710.22092/isfj.2017.110077FAD. MohammadrezaeiB. Majazi AmiriM. FarhangiJournal Article20170619Salmo trutta caspius is an important and economic fish in the Caspian Sea has several
morphological and physiological changes during smoltification. In this study, Hormonal (T3,
T4 & Cortisol) and ionic (Na+, Cl- & K+) changes in the serum were determined during the
period of smoltification in 5, 10, 15 & 20g of hatchery reared salmo trutta caspius in different
seasons (spring, summer, autumn & winter). Hormones were measured by Eliza and Radio
Immuno Assay, Na+ and K+ using flame photometer, Cl- by colorimeter. T3 and T4 were quite
high in spring, especially in the juvenile of 20g. Cortisol was quite low in spring and summer
in all of weight groups. Ionic changes showed no significant differences with weight, but it
was significantly different among seasons. This result suggests that analyzing the plasma
thyroid hormones and ionic provide useful information about the optimal time of transferring
Caspian Sea trout from fresh water to sea water. It is concluded that the juvenile fish of 20g
shows a better smoltification process in the spring.Salmo trutta caspius is an important and economic fish in the Caspian Sea has several
morphological and physiological changes during smoltification. In this study, Hormonal (T3,
T4 & Cortisol) and ionic (Na+, Cl- & K+) changes in the serum were determined during the
period of smoltification in 5, 10, 15 & 20g of hatchery reared salmo trutta caspius in different
seasons (spring, summer, autumn & winter). Hormones were measured by Eliza and Radio
Immuno Assay, Na+ and K+ using flame photometer, Cl- by colorimeter. T3 and T4 were quite
high in spring, especially in the juvenile of 20g. Cortisol was quite low in spring and summer
in all of weight groups. Ionic changes showed no significant differences with weight, but it
was significantly different among seasons. This result suggests that analyzing the plasma
thyroid hormones and ionic provide useful information about the optimal time of transferring
Caspian Sea trout from fresh water to sea water. It is concluded that the juvenile fish of 20g
shows a better smoltification process in the spring.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Evaluation of some biofilter substrates in freshwater recirculation systemEvaluation of some biofilter substrates in freshwater recirculation system12913611007810.22092/isfj.2017.110078FAA. NekuiefardR. ManaffarA.A. Motallebi MoghanjooeiM. SharifianJournal Article20170619The nitrifying bacteria acting on various biofilter substrates including: scoria, P.V.C., marble and
zeolite were evaluated. The experiment started with manufacture of a recirculation system with 130
liter fresh water volume in 7 l.min-1 flow rate in 3 replicate for every treatment. The volume of each
biofilter was 60Ã40Ã30cm and after achievement to stable environmental conditions the nitrifying
bacteria at a concentration of 105ml-1were inoculated in each biofilters. Daily 3-10mg.l-1 of NH4Cl
was added to biofilters. The procedure was conducted by exposure of Ammonium and Nitrate
concentrations and pH in biofilters. The results showed that the Scoria can be reliable substrate as
biofilter, in which provide the best substrate for the growth and attachment of the nitrifying
bacteria which can reduce the retention time of a biofilter from 40-60 to 15 days.The nitrifying bacteria acting on various biofilter substrates including: scoria, P.V.C., marble and
zeolite were evaluated. The experiment started with manufacture of a recirculation system with 130
liter fresh water volume in 7 l.min-1 flow rate in 3 replicate for every treatment. The volume of each
biofilter was 60Ã40Ã30cm and after achievement to stable environmental conditions the nitrifying
bacteria at a concentration of 105ml-1were inoculated in each biofilters. Daily 3-10mg.l-1 of NH4Cl
was added to biofilters. The procedure was conducted by exposure of Ammonium and Nitrate
concentrations and pH in biofilters. The results showed that the Scoria can be reliable substrate as
biofilter, in which provide the best substrate for the growth and attachment of the nitrifying
bacteria which can reduce the retention time of a biofilter from 40-60 to 15 days.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121Evaluation inhibitory effect of essential oil Savory (Satureja hortensis) in food fishEvaluation inhibitory effect of essential oil Savory (Satureja hortensis) in food fish13714411007910.22092/isfj.2017.110079FAL. Yazdanpanah GoharriziA. SepahdariE. SharifpourM. SharifrohaniD. DarvishiJournal Article20170619Aflatoxins are a group of fungal metabolites that are produced by the growth of fungi on
food. These toxins cause illness in animals and humans, and are important in economic and
humans health. In this investigation, inhibitory effects of savory (Satureja hortensis L.)
essential oil were evaluated on the growth of Aspergillus flavus in fish food. A gas
chromatograph apparatus linked to a mass spectrometer (GC/MS) was used to identify the
effective components in Satureja hortensis essential oil after extraction. Essential oils against
Aspergillus flavus incubated in PDA media and antifungal properties of essential oil Satureja
hortensis was investigated. About of 300g of food samples was weighted and samples were
sterilized by autoclave. Fungal suspension (3cc) was spraied into the feed samples, and
various concentrations of essential oils (0, 300, 400, 500, 600ppm) added to samples. The
samples were incubated at temperature of (±SD) 28±2°C. After 20-40 and 60 days period,
randomly, some sampled were taken from containers and the production of aflatoxin B1, B2,
G1, and G2 was measured in the laboratory. This result confirms that 500ppm concentrations
of oil savory have antifungal properties against Aspergillus flavus.Aflatoxins are a group of fungal metabolites that are produced by the growth of fungi on
food. These toxins cause illness in animals and humans, and are important in economic and
humans health. In this investigation, inhibitory effects of savory (Satureja hortensis L.)
essential oil were evaluated on the growth of Aspergillus flavus in fish food. A gas
chromatograph apparatus linked to a mass spectrometer (GC/MS) was used to identify the
effective components in Satureja hortensis essential oil after extraction. Essential oils against
Aspergillus flavus incubated in PDA media and antifungal properties of essential oil Satureja
hortensis was investigated. About of 300g of food samples was weighted and samples were
sterilized by autoclave. Fungal suspension (3cc) was spraied into the feed samples, and
various concentrations of essential oils (0, 300, 400, 500, 600ppm) added to samples. The
samples were incubated at temperature of (±SD) 28±2°C. After 20-40 and 60 days period,
randomly, some sampled were taken from containers and the production of aflatoxin B1, B2,
G1, and G2 was measured in the laboratory. This result confirms that 500ppm concentrations
of oil savory have antifungal properties against Aspergillus flavus.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-13542132012112114514811008010.22092/isfj.2017.110080FAJournal Article20170619Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135421320121121The new record of the spotted catfish Arius maculates (Thunberg 1792) from Persian Gulf, IranThe new record of the spotted catfish Arius maculates (Thunberg 1792) from Persian Gulf, Iran14915411008110.22092/isfj.2017.110081FAP. ZareJournal Article20170619The species Arius maculates (Thunberg 1792) (Siluriformes, Ariidae) is recorded for the
first time from the muddy shores of the inter-tidal zone of Bandar Abbas, Persian Gulf, Iran in
February 2011. In this study, the morphological features of Arius maculates are described.
This species has previously been recorded from Gulf of Oman to Indonesia, north to Japan
(locality type). This finding considerably extends our knowledge of the distribution of Arius
maculates.The species Arius maculates (Thunberg 1792) (Siluriformes, Ariidae) is recorded for the
first time from the muddy shores of the inter-tidal zone of Bandar Abbas, Persian Gulf, Iran in
February 2011. In this study, the morphological features of Arius maculates are described.
This species has previously been recorded from Gulf of Oman to Indonesia, north to Japan
(locality type). This finding considerably extends our knowledge of the distribution of Arius
maculates.