Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023The embryonic development and formation of Bunnei (Barbus sharpeyi Gunther, 1874)The embryonic development and formation of Bunnei (Barbus sharpeyi Gunther, 1874)11211014310.22092/isfj.2017.110143FAS. AhmadiM. KhodadadiL. Roomiani,R. Hakimi MofradJournal Article20170619Understanding the embryogenesis of Bunni is a useful tool for finding the time and suitable
environmental conditions for spawning, growth requirements and use of techniques to
increase the growth rate and survival. The stages of embryonic development for Bunnei
(Barbus sharpeyi) was investigated in 22°C. The reared brood stocks were spawned in
captivity condition. The oocytes were spherical, brown and very adhesive. The cleavage was
fast and the first division was recorded within 15-20 minutes after fertilization. The
blastulation and gastrulation were investigated in 7:40 and 13:10 hours after fertilization with
the yolk sac diameter of 1.18±0.061mm (Mean±SD), respectively. The organogenesis was
started 21 hours after the fertilization, when the blastopore was closed and notochord was
formed. The embryonic stages were continued by appearance the brain, the somites and the
melanophores on the yolk sac. The heart was beaten and shown the blood circulation 65 hours
after the fertilization. The head, tail and pectoral fins were moved frequently. The embryo
reached to pre hatching and final hatching 79:10 and 84:10 hours after fertilization,
respectively, and the embryo existed from corion with the length of about 5.29±0/121mm
(Mean±SD).Understanding the embryogenesis of Bunni is a useful tool for finding the time and suitable
environmental conditions for spawning, growth requirements and use of techniques to
increase the growth rate and survival. The stages of embryonic development for Bunnei
(Barbus sharpeyi) was investigated in 22°C. The reared brood stocks were spawned in
captivity condition. The oocytes were spherical, brown and very adhesive. The cleavage was
fast and the first division was recorded within 15-20 minutes after fertilization. The
blastulation and gastrulation were investigated in 7:40 and 13:10 hours after fertilization with
the yolk sac diameter of 1.18±0.061mm (Mean±SD), respectively. The organogenesis was
started 21 hours after the fertilization, when the blastopore was closed and notochord was
formed. The embryonic stages were continued by appearance the brain, the somites and the
melanophores on the yolk sac. The heart was beaten and shown the blood circulation 65 hours
after the fertilization. The head, tail and pectoral fins were moved frequently. The embryo
reached to pre hatching and final hatching 79:10 and 84:10 hours after fertilization,
respectively, and the embryo existed from corion with the length of about 5.29±0/121mm
(Mean±SD).Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Antibacterial activity of the brown algae (Sargassum glaucescens) ethanolic and aqueous extracts from Chabahar coasts, Oman Sea, IranAntibacterial activity of the brown algae (Sargassum glaucescens) ethanolic and aqueous extracts from Chabahar coasts, Oman Sea, Iran132011014410.22092/isfj.2017.110144FAJ. PeymaniA. GharaeiM. GhaffariA. TaheriJournal Article20170619<span>The widespread uses of antibiotics have been resulted in resistant strains of microorganisms and increasing of worldwide antibiotic resistance. Thus the investigations on new natural antibacterial agents as new drugs are important. According to the previous researches, some multicellular marine algae have significant antibacterial properties. In the present study, antibacterial effects of organic and aqueous extracts of Sargassum glaucescens (collected from Chabahar’s coast, Oman Sea, Iran) were tested on three strains of Gram-negative bacteria: E. coli, Proteus vulgaris, Vibrio cholerae and two strains of Gram-positive bacteria: Listeria monocytogenes and Staphylococcus aureus. Extractions were obtained by immersion method after 48 hours. Antibacterial effects were investigated by the disk diffusion method and serial dilutions in tube to determine the minimum inhibitory concentration. The ethanolic extract showed the largest impact on the L. monocytogenes with significant di.....</span><span>The widespread uses of antibiotics have been resulted in resistant strains of microorganisms and increasing of worldwide antibiotic resistance. Thus the investigations on new natural antibacterial agents as new drugs are important. According to the previous researches, some multicellular marine algae have significant antibacterial properties. In the present study, antibacterial effects of organic and aqueous extracts of Sargassum glaucescens (collected from Chabahar’s coast, Oman Sea, Iran) were tested on three strains of Gram-negative bacteria: E. coli, Proteus vulgaris, Vibrio cholerae and two strains of Gram-positive bacteria: Listeria monocytogenes and Staphylococcus aureus. Extractions were obtained by immersion method after 48 hours. Antibacterial effects were investigated by the disk diffusion method and serial dilutions in tube to determine the minimum inhibitory concentration. The ethanolic extract showed the largest impact on the L. monocytogenes with significant di.....</span>Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Physical and chemical factors determination of Mohammadiye channel water in order to aquaculture purposesPhysical and chemical factors determination of Mohammadiye channel water in order to aquaculture purposes213311014510.22092/isfj.2017.110145FAM. RaminM. DoustdarH. NasrolahzadehF. VahediJournal Article20170619In this survey the Physical and Chemical factors were studied in mohammadiye
channel extending Eslamshahr, Robat karim and Shahriar cities of Tehran Province during the
2011 in agriculture period (May, June, July, August, September, October) Sampling were
done from 5 different stations .The measured factors were temperature,DO,
Cl,HCO3,COD,BOD,CO2,Mg,⦠The analysis of Physical and Chemical factors of
Mohammadiye channel water showed that except water temperature, all factors in comparison
to the standards were at the standard level, So they are suitable for aquaculture.
Due to mean of water temperature that is high from middle of Spring to the end of summer , It
is suitable for culture of warmwater fishes and because of low water temperature in the station
No1 It is recommended for culture of coldwater fishes by using of 50-70gr of young fishesIn this survey the Physical and Chemical factors were studied in mohammadiye
channel extending Eslamshahr, Robat karim and Shahriar cities of Tehran Province during the
2011 in agriculture period (May, June, July, August, September, October) Sampling were
done from 5 different stations .The measured factors were temperature,DO,
Cl,HCO3,COD,BOD,CO2,Mg,⦠The analysis of Physical and Chemical factors of
Mohammadiye channel water showed that except water temperature, all factors in comparison
to the standards were at the standard level, So they are suitable for aquaculture.
Due to mean of water temperature that is high from middle of Spring to the end of summer , It
is suitable for culture of warmwater fishes and because of low water temperature in the station
No1 It is recommended for culture of coldwater fishes by using of 50-70gr of young fishesAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Study on banana prawn (Fenneropenaeus merguiensis) movement in the Hormozgan waters in the northern Persian GulfStudy on banana prawn (Fenneropenaeus merguiensis) movement in the Hormozgan waters in the northern Persian Gulf344011014610.22092/isfj.2017.110146FAG. A. ZarshenasN. NiamaimandiK. PazirJournal Article20170619This research was carried out in Strait of Hormuz in 2020. The present study aimed to
investigate the migration and growth pattern of tagged shrimps in the study area.
Bloodstock shrimps were reared in June (2010). About 50000 juvenile shrimps were tagged
by red color florescent elastomeric tag and released in the estuaries of Tiab, Kolahi and
Koolgan at night in July 2010. Local people were informed through posters describing the
tagging program, as well as articles published in a local magazine. A tagging program film
was broadcasted on local television three times prior to the shrimp fishing season and during
shrimp catch period.
In the catch season, September and November months, 11 tagged prawns (0.022%) were
recaptured. Movement direction of tagged prawns was northwesterly of deeper waters of
releasing areas. Speed was recorded at 0.33-0.33 km/day and tagged prawns were recaptured
46 km from the released locations. The mean (±SD) weight of released banana prawns and the
average weight of the recaptured prawns were1.2±0.2 g and 22.06±4.9 g, respectively. The
growth rate of recaptured prawns was 16-26 g and growth speed was 0.88-1.41 g per week.
In the study area, the major sea current was from the Oman Sea to the Persian Gulf through
Strait of Hormuz probably influencing the pattern of banana prawn movement in HormozganThis research was carried out in Strait of Hormuz in 2020. The present study aimed to
investigate the migration and growth pattern of tagged shrimps in the study area.
Bloodstock shrimps were reared in June (2010). About 50000 juvenile shrimps were tagged
by red color florescent elastomeric tag and released in the estuaries of Tiab, Kolahi and
Koolgan at night in July 2010. Local people were informed through posters describing the
tagging program, as well as articles published in a local magazine. A tagging program film
was broadcasted on local television three times prior to the shrimp fishing season and during
shrimp catch period.
In the catch season, September and November months, 11 tagged prawns (0.022%) were
recaptured. Movement direction of tagged prawns was northwesterly of deeper waters of
releasing areas. Speed was recorded at 0.33-0.33 km/day and tagged prawns were recaptured
46 km from the released locations. The mean (±SD) weight of released banana prawns and the
average weight of the recaptured prawns were1.2±0.2 g and 22.06±4.9 g, respectively. The
growth rate of recaptured prawns was 16-26 g and growth speed was 0.88-1.41 g per week.
In the study area, the major sea current was from the Oman Sea to the Persian Gulf through
Strait of Hormuz probably influencing the pattern of banana prawn movement in HormozganAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Incidence of streptococcusis in rainbow trout (Onchorhynchus mykiss ) farms in Haraz River in Mazandaran Province, IranIncidence of streptococcusis in rainbow trout (Onchorhynchus mykiss ) farms in Haraz River in Mazandaran Province, Iran415011014710.22092/isfj.2017.110147FAA. SepahdariA. S. SaeediS. KakoulakiF. Habibi KotanaeeA. R. BabaalianJournal Article20170619One of the most important bacterial fish diseases which have caused some outbreaks in
rainbow trout farms in Iran is streptococcusis .The farmers have been suffering from huge
economical losses due to the disease outbreak in different rainbow trout farms in Iran. The
aim of present study was to determine the rate of streptococcusis incidence in different growth
stage in the farmed rainbow trout in Haraz River in Mazandaran Province, Iran. Fish
specimens along with water samples were collected from 10 haphazardly selected fish farms
on a monthly basis throughout a year. After clinical observations, isolation and recognition of
strep strains were undertaken using biochemical and PCR tests. The results showed that 4.6%
of juvenile fish showed clinical sings of streptococcusis while only 0.7% had strep.
contamination. These rates in adult specimens were 8.9 and 1 percent, respectively. The main
isolated bacterial strain was Streptococcus uberisOne of the most important bacterial fish diseases which have caused some outbreaks in
rainbow trout farms in Iran is streptococcusis .The farmers have been suffering from huge
economical losses due to the disease outbreak in different rainbow trout farms in Iran. The
aim of present study was to determine the rate of streptococcusis incidence in different growth
stage in the farmed rainbow trout in Haraz River in Mazandaran Province, Iran. Fish
specimens along with water samples were collected from 10 haphazardly selected fish farms
on a monthly basis throughout a year. After clinical observations, isolation and recognition of
strep strains were undertaken using biochemical and PCR tests. The results showed that 4.6%
of juvenile fish showed clinical sings of streptococcusis while only 0.7% had strep.
contamination. These rates in adult specimens were 8.9 and 1 percent, respectively. The main
isolated bacterial strain was Streptococcus uberisAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Comparison of four RNA isolating methods for identification of spring viraemia of carp virus (SVCV)Comparison of four RNA isolating methods for identification of spring viraemia of carp virus (SVCV)515911014810.22092/isfj.2017.110148FAS.R.S. MortezaeiM. AlishahiM. R. SeifiM. QhasemiJournal Article20170619Spring viraemia of carp virus (SVCV) , a negative sense single stranded RNA virus of the
family Rhabdoviridae, is the causative agent of a highly contagious SVC disease that
primarily affects the common carp ( Cyprinus carpio), an economically important fresh water
fish species with world-wide distribution.SVCV has also been reported to cause disease in
other fishes such as Poeciliidae, Esocida , Centrarchidae , Siluridae and salmonidae . There
are several diagnostic tests for the detection of SVC virus,however, the tests have not been
validated. The reverse transcriptase â polymerase chain reaction (RT-PCR) techniques have
been developed and validated representing a powerful tool for detection of RNA. One of the
most important aspects isolating RNA is to prevent degradation of the RNA during the
isolation procedure. In this study, we explored the efficiency of protocols for RNA isolation
from the SVCV strain 56/70.For RNA isolation, we compared four protocols, two guanidine
isotiocyanate phenol â chloroform based protocols ( RNX â Plus Iran , Iq2000 kit Taiwan )
and two column based protocols ( Cinnapure RNA Iran , high pure viral RNA kit , Roche
Germany ) that were commercially available. The results showed that the column based
protocols, Roche method and Cinapure performed better than other methods with the yields
of 31.76 ng/μl, 16/21 ng/μl, respectively. Each protocol yielded good quality of total RNA
bands (480 bp) being observed in agarose gel electrophoreses but was not observed in IQ2000
kit. Amount of total RNA isolated was lower for IQ2000 kit Protocol. Further, the RNA
being extracted from SVC by column based protocol method were resulted in successful
amplified using RT-PCR methodSpring viraemia of carp virus (SVCV) , a negative sense single stranded RNA virus of the
family Rhabdoviridae, is the causative agent of a highly contagious SVC disease that
primarily affects the common carp ( Cyprinus carpio), an economically important fresh water
fish species with world-wide distribution.SVCV has also been reported to cause disease in
other fishes such as Poeciliidae, Esocida , Centrarchidae , Siluridae and salmonidae . There
are several diagnostic tests for the detection of SVC virus,however, the tests have not been
validated. The reverse transcriptase â polymerase chain reaction (RT-PCR) techniques have
been developed and validated representing a powerful tool for detection of RNA. One of the
most important aspects isolating RNA is to prevent degradation of the RNA during the
isolation procedure. In this study, we explored the efficiency of protocols for RNA isolation
from the SVCV strain 56/70.For RNA isolation, we compared four protocols, two guanidine
isotiocyanate phenol â chloroform based protocols ( RNX â Plus Iran , Iq2000 kit Taiwan )
and two column based protocols ( Cinnapure RNA Iran , high pure viral RNA kit , Roche
Germany ) that were commercially available. The results showed that the column based
protocols, Roche method and Cinapure performed better than other methods with the yields
of 31.76 ng/μl, 16/21 ng/μl, respectively. Each protocol yielded good quality of total RNA
bands (480 bp) being observed in agarose gel electrophoreses but was not observed in IQ2000
kit. Amount of total RNA isolated was lower for IQ2000 kit Protocol. Further, the RNA
being extracted from SVC by column based protocol method were resulted in successful
amplified using RT-PCR methodAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Morpho - anatomical studies on red alga Gracilaria corticata in the Persian Gulf and Oman Sea.Morpho - anatomical studies on red alga Gracilaria corticata in the Persian Gulf and Oman Sea.607011014910.22092/isfj.2017.110149FAS. A. TalebzadehS.M. Hesamzade HejaziH. MaghsoodiT. ValinassabJournal Article20170619The speciemns of red algae âGracilaria corticataâ were collected from the rocky intertidal
shores in Bostaneh Port in the Persian Gulf and Lipar in Oman Sea in May, June and August
2013. Anatomical, morphological structures, thallus of vegetative and reproductive samples
were studied. Carpogonial and tetrasporangium stages were determined based on histological
and morphological methods. The female thallus, the Cystocarp with hemispherical structure
through out thallus were observed. The Spermatangial conceptacle was located deeper as the
oval shape in male thallus. The tetrasporophytic stage of diploid samples was determined by
the presence of tetrasporangium oval shape.The speciemns of red algae âGracilaria corticataâ were collected from the rocky intertidal
shores in Bostaneh Port in the Persian Gulf and Lipar in Oman Sea in May, June and August
2013. Anatomical, morphological structures, thallus of vegetative and reproductive samples
were studied. Carpogonial and tetrasporangium stages were determined based on histological
and morphological methods. The female thallus, the Cystocarp with hemispherical structure
through out thallus were observed. The Spermatangial conceptacle was located deeper as the
oval shape in male thallus. The tetrasporophytic stage of diploid samples was determined by
the presence of tetrasporangium oval shape.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023The sub-lethal effects of Cadmium on some gill histopathology in the cultivated Sterlet (Acipenser ruthenus)The sub-lethal effects of Cadmium on some gill histopathology in the cultivated Sterlet (Acipenser ruthenus)718111015010.22092/isfj.2017.110150FAM. OrojaliF. Paykan HeyratiS. Alar DorafshanN. Mahboobi SoofianiJournal Article20170619The sub-lethal effects of water-born Cadmium (Cd) on histopathological changes of gill
tissues were investigated on Sterlet, Acipenser ruthenus. In this research, 60 fish (41.69 ± 0.95
g and 23.98 ± 0.14 cm) were randomly distributed in 4 experimental groups, each with 3
replicates. The fish were exposed to different concentrations of Cd for 21 days under semistatic
experimental condition as: 0, 16, 32 and 64 μg/L Cd. At the end of the experiment, the
gill tissues were fixed in Bouin's solution for histopathological studies. Qualitative and
quantitative changes of the gill tissues were analyzed. Several qualitative changes including
hyperplasia, hypertrophy of epithelial cells and epithelial edema as well as necrosis, rings and
fusion of gill filaments and secondary lamellae were observed in all treated fish including
control group. Quantitative analysis based on organ index (Iorg) showed significant increase in
histopathological changes in the gill tissues coincided by elevation of Cd concentration in the
water. However, there was no significant differences in the organ index between control and
16Cd group.The results of this study demonstrate severe histopathological changes in the gill
tissues after exposing to sub-lethal concentration of water-born Cd under experimental
condition. The results of this study might be used as a guildline for evaluation of
histopahological changes being induced by heavy metals in natural or cultivated environment
for sturgeon fish.The sub-lethal effects of water-born Cadmium (Cd) on histopathological changes of gill
tissues were investigated on Sterlet, Acipenser ruthenus. In this research, 60 fish (41.69 ± 0.95
g and 23.98 ± 0.14 cm) were randomly distributed in 4 experimental groups, each with 3
replicates. The fish were exposed to different concentrations of Cd for 21 days under semistatic
experimental condition as: 0, 16, 32 and 64 μg/L Cd. At the end of the experiment, the
gill tissues were fixed in Bouin's solution for histopathological studies. Qualitative and
quantitative changes of the gill tissues were analyzed. Several qualitative changes including
hyperplasia, hypertrophy of epithelial cells and epithelial edema as well as necrosis, rings and
fusion of gill filaments and secondary lamellae were observed in all treated fish including
control group. Quantitative analysis based on organ index (Iorg) showed significant increase in
histopathological changes in the gill tissues coincided by elevation of Cd concentration in the
water. However, there was no significant differences in the organ index between control and
16Cd group.The results of this study demonstrate severe histopathological changes in the gill
tissues after exposing to sub-lethal concentration of water-born Cd under experimental
condition. The results of this study might be used as a guildline for evaluation of
histopahological changes being induced by heavy metals in natural or cultivated environment
for sturgeon fish.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Evaluation of physiological aspects and molecular identification of Saprolegnia isolates from rainbow trout (Oncorhynchus mykiss) and Caspian trout (Salmo trutta caspius) eggs based on RAPDâPCREvaluation of physiological aspects and molecular identification of Saprolegnia isolates from rainbow trout (Oncorhynchus mykiss) and Caspian trout (Salmo trutta caspius) eggs based on RAPDâPCR829211015110.22092/isfj.2017.110151FAM. GhiasiA. Khosravi,M. Soltani,M. BinaiiI. SharifpourH. Ebrahimzadeh MosaviA. BahonarJournal Article20170619The genus of saprolegnia is one of the most important pathogenic aquatic fungi in farmed and
wild fish. In the present study, fungal infected egss were collected from rainbow trout
(Oncorhyncus mykiss) and Caspian trout (Salmo trutta caspius). After purification, 16 isolates
were obtained (8 isolates from rainbow trout and 8 isolates from Caspian salmon,
respectively). The isolates were then coded as R2 â R9 (rainbow trout) and S2 â S9 (Caspian
trout).The registered DNA for S. parasitica (ACTT # 200048) and S. diclina (ACTT #
4206) were used and coded as R1 and S1, respectively. Based on the RAPD profile obtained
all samples were divided to 3 groups and members of each group had more than 90%
similarity among themselves. According to matrix of similarity and reference strains, the
isolates were classified as three groups. Therefore, all of isolates in group 1 and 3 were S.
parasitica and S. diclina, respectively, and the members of group 2 were known as
Saprolegnia sp. The results of thermal resistance assessment showed that the isolates of
rainbow trout and Caspian salmon eggs had slow growth in the temperature between 18 â 20
°C. Thus, R2 and S8 isolates had the lowest radial growth compared to other isolates. The
isolates categorized in S. parasitica (group 1) created secoundry zoospores but not observed
in two other groups. Thus, catenulated gamme was found in 78% and 55.55% isolates of
rainbow trout and Caspian trout eggs, respectively.
This study indicated that molecular methods were the best methods for identification of
Saprolegnia spp. and it could be applied as a supplementary confirming method.The genus of saprolegnia is one of the most important pathogenic aquatic fungi in farmed and
wild fish. In the present study, fungal infected egss were collected from rainbow trout
(Oncorhyncus mykiss) and Caspian trout (Salmo trutta caspius). After purification, 16 isolates
were obtained (8 isolates from rainbow trout and 8 isolates from Caspian salmon,
respectively). The isolates were then coded as R2 â R9 (rainbow trout) and S2 â S9 (Caspian
trout).The registered DNA for S. parasitica (ACTT # 200048) and S. diclina (ACTT #
4206) were used and coded as R1 and S1, respectively. Based on the RAPD profile obtained
all samples were divided to 3 groups and members of each group had more than 90%
similarity among themselves. According to matrix of similarity and reference strains, the
isolates were classified as three groups. Therefore, all of isolates in group 1 and 3 were S.
parasitica and S. diclina, respectively, and the members of group 2 were known as
Saprolegnia sp. The results of thermal resistance assessment showed that the isolates of
rainbow trout and Caspian salmon eggs had slow growth in the temperature between 18 â 20
°C. Thus, R2 and S8 isolates had the lowest radial growth compared to other isolates. The
isolates categorized in S. parasitica (group 1) created secoundry zoospores but not observed
in two other groups. Thus, catenulated gamme was found in 78% and 55.55% isolates of
rainbow trout and Caspian trout eggs, respectively.
This study indicated that molecular methods were the best methods for identification of
Saprolegnia spp. and it could be applied as a supplementary confirming method.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Genetic variation in farmed population of Rainbow trout (Onchorhynchus mykiss) based on microsatellite markers in IranGenetic variation in farmed population of Rainbow trout (Onchorhynchus mykiss) based on microsatellite markers in Iran9310611015210.22092/isfj.2017.110152FAE. Einollah GorjipoorSajad NazariJournal Article20170619A total of 64 specimens of rainbow trout (Onchorhynchus mykiss) from 3 different regions in
Iran were collected. About 2-3 g of caudal fin samples were removed from each specimen and
preserved in absolute ethyl alcohol and transferred to the genetic laboratory. Genomic DNA
was extracted using the phenol-chloroform method and then DNA content and quality was
determined using spectrophotometry and agarose gel electrophoresis, respectively.
Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 8 pairs
of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel
and stained with silver nitrate. Following the scoring of alleles, all parameters including
effective number of alleles, observed and expected heterozygosity, shanon index, Hardy-
Weinberg equilibrium test and FST were calculated using AMOVA analysis in the GenAlex
and Popgene programs. The results showed that 8 pairs of microsatellite primers were
polymorphic. In total, alleles were determined with the range size of 64-280 bp. The locus
OtsG 249 had maximum number of allele (9) and loci OtsG 432 and OtsG 474 had minimum
number of allele (2). The observed heterozygosity was between 0.869 and 0.916. Hardy-
Weinberg departure was observed for most loci from all farms and were disequilibrium. The
Fst results showed that maximum FST (0.079) were between farms in Tehran and Yasuj and
minimum (0.041) were between farms in Hamadan and Yasuj. Based on the results of
AMOVA analysis, significant differences were detected between all farms. The results
suggest that the unique genetic variation of rainbow trout in hatchery farms of Iran represents
a highly valuable genetic resource and provide useful information for creating a based
population in the future breeding programs.A total of 64 specimens of rainbow trout (Onchorhynchus mykiss) from 3 different regions in
Iran were collected. About 2-3 g of caudal fin samples were removed from each specimen and
preserved in absolute ethyl alcohol and transferred to the genetic laboratory. Genomic DNA
was extracted using the phenol-chloroform method and then DNA content and quality was
determined using spectrophotometry and agarose gel electrophoresis, respectively.
Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 8 pairs
of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel
and stained with silver nitrate. Following the scoring of alleles, all parameters including
effective number of alleles, observed and expected heterozygosity, shanon index, Hardy-
Weinberg equilibrium test and FST were calculated using AMOVA analysis in the GenAlex
and Popgene programs. The results showed that 8 pairs of microsatellite primers were
polymorphic. In total, alleles were determined with the range size of 64-280 bp. The locus
OtsG 249 had maximum number of allele (9) and loci OtsG 432 and OtsG 474 had minimum
number of allele (2). The observed heterozygosity was between 0.869 and 0.916. Hardy-
Weinberg departure was observed for most loci from all farms and were disequilibrium. The
Fst results showed that maximum FST (0.079) were between farms in Tehran and Yasuj and
minimum (0.041) were between farms in Hamadan and Yasuj. Based on the results of
AMOVA analysis, significant differences were detected between all farms. The results
suggest that the unique genetic variation of rainbow trout in hatchery farms of Iran represents
a highly valuable genetic resource and provide useful information for creating a based
population in the future breeding programs.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Effect of different dietary protein and energy levels on growth of juvenile Beluga (Huso huso)Effect of different dietary protein and energy levels on growth of juvenile Beluga (Huso huso)10711811015310.22092/isfj.2017.110153FAM. MohseniA. AmirkhaniM. H. Seyed HassaniH. R. PouraliJournal Article20170619A 2 Ã 4 factorial design was conducted to study the effects of dietary protein and energy
levels on growth performance of juvenile beluga (Huso huso) with average weight of 49.85 ±
1.64 g. Experiments were conducted with two dietary protein levels (40 and 45%) and four
dietary energy level (18.5, 19.8, 21.1 and 22.4 MJ/kg diet) by ratio P/E (18.03 to 24.14
mg/kj). Fish were fed the experimental diets for 110 days. In each of the protein levels (40
and 45%), the feed efficiency, body weight increase, Protein efficiency ratio, specific growth
rate, final body weight and total feed intake of fish improved significantly as dietary energy
levels increased (P⤠0.05). Growth of fish fed high- energy diets was significantly higher than
those of fish fed low- energy diets at 40 and 45% dietary protein levels (P⤠0.05). Protein
content of carcass increased when dietary protein increased, but there was no significant
different (P⥠0.05) between 40 and 45 % protein. Lipid content of fish fed diet high- energy
level was significantly higher than those of fish fed low dietary energy (P⤠0.05). So the
maximum growth and weight( 22.4 ± 5.3 gr) was observed in treatment, that were fed diets
containing 45% protein & 22.4 mega joule crude energy. Considering that no significant
difference were affected for among the diet for the parameters studied, the diet containing 40
% protein, 21 % fat and 21.1 mega Joule crude energy per kilogram diet with a P/E ratio of 20
mg protein per kilo joule from a good quality source is a suitable diet in terms of physiology
and economy can be considered a suitable diet to produce maximum growth in juvenile
beluga in the weight class 49 to 200g.A 2 Ã 4 factorial design was conducted to study the effects of dietary protein and energy
levels on growth performance of juvenile beluga (Huso huso) with average weight of 49.85 ±
1.64 g. Experiments were conducted with two dietary protein levels (40 and 45%) and four
dietary energy level (18.5, 19.8, 21.1 and 22.4 MJ/kg diet) by ratio P/E (18.03 to 24.14
mg/kj). Fish were fed the experimental diets for 110 days. In each of the protein levels (40
and 45%), the feed efficiency, body weight increase, Protein efficiency ratio, specific growth
rate, final body weight and total feed intake of fish improved significantly as dietary energy
levels increased (P⤠0.05). Growth of fish fed high- energy diets was significantly higher than
those of fish fed low- energy diets at 40 and 45% dietary protein levels (P⤠0.05). Protein
content of carcass increased when dietary protein increased, but there was no significant
different (P⥠0.05) between 40 and 45 % protein. Lipid content of fish fed diet high- energy
level was significantly higher than those of fish fed low dietary energy (P⤠0.05). So the
maximum growth and weight( 22.4 ± 5.3 gr) was observed in treatment, that were fed diets
containing 45% protein & 22.4 mega joule crude energy. Considering that no significant
difference were affected for among the diet for the parameters studied, the diet containing 40
% protein, 21 % fat and 21.1 mega Joule crude energy per kilogram diet with a P/E ratio of 20
mg protein per kilo joule from a good quality source is a suitable diet in terms of physiology
and economy can be considered a suitable diet to produce maximum growth in juvenile
beluga in the weight class 49 to 200g.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Identification of a hermit crab, Clibanarius signatus, in Hormuz Island abundance, sex ratio and shell selection behaviorsIdentification of a hermit crab, Clibanarius signatus, in Hormuz Island abundance, sex ratio and shell selection behaviors11912711015410.22092/isfj.2017.110154FAN. KheirabadiJ. SeyfabadiF. OwfiA. MahvariJournal Article20170619In order to establish a complete list of the hermit crab species in the southern coast of Iran and
also determine some of their biological characteristics in the intertidal zones of Hormuz
Island, a seasonal sampling collection was conducted in 6 intertidal stations during the years
2010â2011. The 542 collected samples were found to be Clibanarius signatus, which
identified in the island. Comparing the samples with the metatypical hermit crab samples in
the Zoological Museum, University of Tehran, verified the species. The one ways ANOVA
with LSD test showed a significant difference (p < 0.05) in the abundance of the species in
some stations, but seasonal changes did not significantly affect the abundance. Male-female
sex ratio was 1/52 male: 1 female. Seasonal study of sexes (male, non-ovigerous female,
ovigerous female) confirmed that species did not have a particular spawning season,
nevertheless the spawning increased in summer. The shell selection behaviors showed that the
hermit crab uses the shells of 36 gastropod species (24 genera) for protecting its abdominal
section.In order to establish a complete list of the hermit crab species in the southern coast of Iran and
also determine some of their biological characteristics in the intertidal zones of Hormuz
Island, a seasonal sampling collection was conducted in 6 intertidal stations during the years
2010â2011. The 542 collected samples were found to be Clibanarius signatus, which
identified in the island. Comparing the samples with the metatypical hermit crab samples in
the Zoological Museum, University of Tehran, verified the species. The one ways ANOVA
with LSD test showed a significant difference (p < 0.05) in the abundance of the species in
some stations, but seasonal changes did not significantly affect the abundance. Male-female
sex ratio was 1/52 male: 1 female. Seasonal study of sexes (male, non-ovigerous female,
ovigerous female) confirmed that species did not have a particular spawning season,
nevertheless the spawning increased in summer. The shell selection behaviors showed that the
hermit crab uses the shells of 36 gastropod species (24 genera) for protecting its abdominal
section.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023Effect of parasite infection with Posthodiplostomum cuticola (Nordmann, 1832) on fish growth rate in Zaringol Stream in Golestan ProvinceEffect of parasite infection with Posthodiplostomum cuticola (Nordmann, 1832) on fish growth rate in Zaringol Stream in Golestan Province12813311015510.22092/isfj.2017.110155FAZ. Mazaheri KohanestaniR. GhorbaniA. HajimoradlooS. YulghiA. HoseiniJournal Article20170619This study investigated the effect of parasite infection with Posthodiplostomum cuticola on fish
growth rate in Zaringol Stream in Golestan Province. A total number of 447 fish specimens (173
Capoeta capoeta gracilis, 111 Alburnoides eichwaldii, 131 Paracobitis malapterura, 32 Neogobius
fluviatilis) were captured by electrofishing during December 2009 to September 2010. Total
number of parasite on fish body was counted by investigating internal organ and surface of skin and
branch. Results showed that slope of regression curves and condition factor were significantly lower
in infected C. c. gracilis. N. fluviatilis was the most abundant infected fish in Zarringol Stream,. It
seems that it's feeding dependence on benthos makes this fish more susceptible to infection by
parasiteThis study investigated the effect of parasite infection with Posthodiplostomum cuticola on fish
growth rate in Zaringol Stream in Golestan Province. A total number of 447 fish specimens (173
Capoeta capoeta gracilis, 111 Alburnoides eichwaldii, 131 Paracobitis malapterura, 32 Neogobius
fluviatilis) were captured by electrofishing during December 2009 to September 2010. Total
number of parasite on fish body was counted by investigating internal organ and surface of skin and
branch. Results showed that slope of regression curves and condition factor were significantly lower
in infected C. c. gracilis. N. fluviatilis was the most abundant infected fish in Zarringol Stream,. It
seems that it's feeding dependence on benthos makes this fish more susceptible to infection by
parasiteAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135422420141023A new record of the puffer fish Takifugu oblongus (Bloch, 1786) from the northern Persian Gulf, IranA new record of the puffer fish Takifugu oblongus (Bloch, 1786) from the northern Persian Gulf, Iran13413811015610.22092/isfj.2017.110156FAM. NaderiP. ZareE. AzvarD. PitassyJournal Article20170619The new record of a puffer fish âTakifugu oblongus Bloch, 1786â (Tetraodontiformes, Tetraodontidae) is recorded for the first time the muddy shores of the inter-tidal zone of Bandar-e-Abbas city, in the northern Persian Gulf, Iran in March 2011. The morphological features of Takifugu oblongus are described. This species has previously been recorded from Indo-West Pacific, South Africa to Indonesia, Japan, China, and Korea (locality type). This finding considerably extends our knowledge of the distribution of Takifugu oblongus.The new record of a puffer fish âTakifugu oblongus Bloch, 1786â (Tetraodontiformes, Tetraodontidae) is recorded for the first time the muddy shores of the inter-tidal zone of Bandar-e-Abbas city, in the northern Persian Gulf, Iran in March 2011. The morphological features of Takifugu oblongus are described. This species has previously been recorded from Indo-West Pacific, South Africa to Indonesia, Japan, China, and Korea (locality type). This finding considerably extends our knowledge of the distribution of Takifugu oblongus.