Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Study on mesh breaking strength of conical lift net before and after usageStudy on mesh breaking strength of conical lift net before and after usage1511034110.22092/isfj.2017.110341FAMobin MoshkabadiSaeid GorginHassan FazliAli Akbar GhareaghajiJournal Article20170619Conical lift nets catch kilka fish in Iran. Conical lift nets are usually made of polyamide 6.6 (nylon) with 210D/ 9 number. One of the main problems of fishermen is determining the right time to change fishing nets. Unfortunately, due to lack of data they do not change their nets at the right time. Data were analyses using and The mean values of breaking strength data were compared using one-way ANOVA and Duncan's test. The results showed that there is significant differences between new nets and nets which is used for 10 months (P<0.05).Conical lift nets catch kilka fish in Iran. Conical lift nets are usually made of polyamide 6.6 (nylon) with 210D/ 9 number. One of the main problems of fishermen is determining the right time to change fishing nets. Unfortunately, due to lack of data they do not change their nets at the right time. Data were analyses using and The mean values of breaking strength data were compared using one-way ANOVA and Duncan's test. The results showed that there is significant differences between new nets and nets which is used for 10 months (P<0.05).Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Measurement of some heavy metals concentration in live feed commonly used
in aquacultureMeasurement of some heavy metals concentration in live feed commonly used
in aquaculture71311347910.22092/isfj.2017.113479FAZ. Hashemi HosseinabadiS. SoltanianM. AkhlaghiJournal Article20171024<span>The aim of the current investigation was to determine the concentration of some heavy metals in a number of aquatic invertebraes commnly used as live feed in aquaculture. For this goal, samples of blood worm, sludge worm, earthworm, gammarus, shelled snail, freshwater ghost shrimp and tiny marine shrimp were prepared from aquatic environment or from imported live feeds. Samples were digested chemically and the atomic absorption were recorded as µg/g dry weight. Results showed that the highest level of heavy metals concentration were detected in live feeds as follows: iron (213.4±10.2), cadmium (1.15±0.013) and nickel (20.97±3.6) in earthworm; zinc (105.71±5.8), chrome (19.63±5.3) in sludge worm; copper (37.34±4.2) in freshwater ghost shrimp and lead (24.71±4.7) in tiny marine shrimp respectively. It is proposed that high rates of heavy metals detected in some of live feeds in the present study may eventually have a severe negative impact on fish and human health conditions. Therefore, .....</span><span>The aim of the current investigation was to determine the concentration of some heavy metals in a number of aquatic invertebraes commnly used as live feed in aquaculture. For this goal, samples of blood worm, sludge worm, earthworm, gammarus, shelled snail, freshwater ghost shrimp and tiny marine shrimp were prepared from aquatic environment or from imported live feeds. Samples were digested chemically and the atomic absorption were recorded as µg/g dry weight. Results showed that the highest level of heavy metals concentration were detected in live feeds as follows: iron (213.4±10.2), cadmium (1.15±0.013) and nickel (20.97±3.6) in earthworm; zinc (105.71±5.8), chrome (19.63±5.3) in sludge worm; copper (37.34±4.2) in freshwater ghost shrimp and lead (24.71±4.7) in tiny marine shrimp respectively. It is proposed that high rates of heavy metals detected in some of live feeds in the present study may eventually have a severe negative impact on fish and human health conditions. Therefore, .....</span>Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Effect of astaxanthin on growth, body chemical composition and some blood serum biochemical indices in grey mullet, Mugil cephalus Linnaeus, 1758Effect of astaxanthin on growth, body chemical composition and some blood serum biochemical indices in grey mullet, Mugil cephalus Linnaeus, 1758152411348010.22092/isfj.2017.113480FAH. Rigi GhazaghA. AbromandS. ZiaeinezhadP. AkbariJournal Article20171024This experiment was conducted to evaluate the effect of astaxanthin on the growth performances (final weight (FW), specific growth ratio (SGR) and condition factor (CF) feed indices (feed conversion rate (FCR), voluntary feed intake (VFI) and protein efficiency ratio (PER)), body chemical (protein, fat, moisture and ash) and some blood serum biochemical parameters (total protein, glucose, triglyceride and cholesterol) of <em>Mugil cephalus</em> for 60 days. The experiment was conducted in a completely randomized design with 120 of <em>Mugil cephalus</em> (with average weight of 11.77±0.07g) in 4 treatments and 3 replicates (n=10 in each replicate) and included: control group without using astaxanthin, an another groups (treatment 2, 3 and 4) the amounts of astaxanthin were 50,100 and 150 mg/kg food. The results showed that at the end of experiment, the highest FW (4.22±0.11g), DGI (1.77±0.51%), the lowest FCR (0.95±0.05), the highest SGR, PER and CF were observed in the diet containing 150 mg /kg astaxanthin (p<0.05). Also, the highest FW and total protein and the lowest triglyceride and cholesterol were observed diets containing 100 and 150 mg /kg astaxanthin. The lowest crude fat was observed in treatments containing astaxanthin (p<0.05). in Finally, diet containing 100 mg/kg astaxanthin could improve growth, feed performances, carcass quality and blood chemical parameters in <em>Mugil cephalus</em>.This experiment was conducted to evaluate the effect of astaxanthin on the growth performances (final weight (FW), specific growth ratio (SGR) and condition factor (CF) feed indices (feed conversion rate (FCR), voluntary feed intake (VFI) and protein efficiency ratio (PER)), body chemical (protein, fat, moisture and ash) and some blood serum biochemical parameters (total protein, glucose, triglyceride and cholesterol) of <em>Mugil cephalus</em> for 60 days. The experiment was conducted in a completely randomized design with 120 of <em>Mugil cephalus</em> (with average weight of 11.77±0.07g) in 4 treatments and 3 replicates (n=10 in each replicate) and included: control group without using astaxanthin, an another groups (treatment 2, 3 and 4) the amounts of astaxanthin were 50,100 and 150 mg/kg food. The results showed that at the end of experiment, the highest FW (4.22±0.11g), DGI (1.77±0.51%), the lowest FCR (0.95±0.05), the highest SGR, PER and CF were observed in the diet containing 150 mg /kg astaxanthin (p<0.05). Also, the highest FW and total protein and the lowest triglyceride and cholesterol were observed diets containing 100 and 150 mg /kg astaxanthin. The lowest crude fat was observed in treatments containing astaxanthin (p<0.05). in Finally, diet containing 100 mg/kg astaxanthin could improve growth, feed performances, carcass quality and blood chemical parameters in <em>Mugil cephalus</em>.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Effect of fish meal replacement with sunflower meal on growth indices and body composition of common carp (Cyprinus carpio) fingerlingsEffect of fish meal replacement with sunflower meal on growth indices and body composition of common carp (Cyprinus carpio) fingerlings253511348110.22092/isfj.2017.113481FAK. JavidrahmdelH. Allaf NoveirianB. FalahatkarA. Babakhani LashkanJournal Article20171024The present study was conducted to determine the effect of fish meal replacement with sunflower meal on growth performance, feed efficiency and body proximate composition of common carp fingerlings. Five experimental diets with replacement levels of 0, 25, 50, 75 and 100% of fish meal with sunflower meal were formulated with equal protein, fat and energy contents. Fish meal proportion of control diet was 28%. Fish with average weight of 3.03 ± 0.36 g were distributed in 15 fiberglass tanks. After 10 weeks of feeding by experimental diets, growth performance and feed efficiency were evaluated and 6 fish per tank were taken for body composition analysis. According to the results, highest final weight was derived in 25% treatment (p<0.05), although differences in 50 and 75% were not significant compared to control (p>0.05), but in 100% treatment significant decline in growth and feed efficiency was observed (p<0.05). Also, there was significant difference in body composition including moisture, protein and fat (p<0.05), but ash content showed no significant difference (p>0.05) among treatments. Results of this study showed, it is possible to replace fish meal with sunflower meal up to 75% in common carp fingerlings diet without negative impacts on growth performance, feed efficiency and body composition.The present study was conducted to determine the effect of fish meal replacement with sunflower meal on growth performance, feed efficiency and body proximate composition of common carp fingerlings. Five experimental diets with replacement levels of 0, 25, 50, 75 and 100% of fish meal with sunflower meal were formulated with equal protein, fat and energy contents. Fish meal proportion of control diet was 28%. Fish with average weight of 3.03 ± 0.36 g were distributed in 15 fiberglass tanks. After 10 weeks of feeding by experimental diets, growth performance and feed efficiency were evaluated and 6 fish per tank were taken for body composition analysis. According to the results, highest final weight was derived in 25% treatment (p<0.05), although differences in 50 and 75% were not significant compared to control (p>0.05), but in 100% treatment significant decline in growth and feed efficiency was observed (p<0.05). Also, there was significant difference in body composition including moisture, protein and fat (p<0.05), but ash content showed no significant difference (p>0.05) among treatments. Results of this study showed, it is possible to replace fish meal with sunflower meal up to 75% in common carp fingerlings diet without negative impacts on growth performance, feed efficiency and body composition.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Studing the effect of aqueous extract of the Persian Gulf carpet anemone (Stichodactyla haddoni) on breast cancer cell line in vitroStuding the effect of aqueous extract of the Persian Gulf carpet anemone (Stichodactyla haddoni) on breast cancer cell line in vitro374611348210.22092/isfj.2017.113482FAZ. MoghadasiSh. JamiliD. ShahbazzadehN. MossafaK. Poshang BagheriJournal Article20171024Numerous proteins, peptides, and chemical agents in the venom of venomous marine animals are biological active molecules with beneficial pharmacological properties. The main goal of this research was to study the cytotoxic effects of the isolated crude venom from the Persian Gulf sea anemone, <em>Stichodactyla haddoni</em> against Breast (MD-MBA231), and Human embryonic kidney (HEK-293) cell lines <em>in vitro</em> through using MTT assay. Samples of <em>S. haddoni</em> were collected from coastal waters of Lark Island in the Persian Gulf at the south of Iran. Extraction of aqueous part from tentacle was performed. The cell line was cultured in complete tissue culture medium. These cells were exposed to different concentration of venom extract through serial dilution of 100, 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78 μg/ml (ratio 1/2) in 24 hour time duration. The results of kruskal-wallis test indicated that the cytotoxic activity of crude venom extract on cell lines were similar in almost all concentrations and significant differences were not observed (p>0.05). IC50 of crude venom extract against breast (MD-MBA231) and normal (HEK-293) cell lines was observed at 4.13, 117.8µg/ml, respectively. Results obtained from this study showed that increasing the concentration of extract led to the increasing death of both cell lines. Comparing the percentage of death between two cell lines at different concentrations indicated that aqueous extract induced approximately 3.16 folds more cell toxicity on cancer cell line rather than on normal cell lines. Therefore, crude venom extract of <em>S. haddoni</em> can be considered as a strong preventive agent for growth reduction of cancer cell lines especially for breast cancer cell line with low toxicity on normal human cells.Numerous proteins, peptides, and chemical agents in the venom of venomous marine animals are biological active molecules with beneficial pharmacological properties. The main goal of this research was to study the cytotoxic effects of the isolated crude venom from the Persian Gulf sea anemone, <em>Stichodactyla haddoni</em> against Breast (MD-MBA231), and Human embryonic kidney (HEK-293) cell lines <em>in vitro</em> through using MTT assay. Samples of <em>S. haddoni</em> were collected from coastal waters of Lark Island in the Persian Gulf at the south of Iran. Extraction of aqueous part from tentacle was performed. The cell line was cultured in complete tissue culture medium. These cells were exposed to different concentration of venom extract through serial dilution of 100, 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78 μg/ml (ratio 1/2) in 24 hour time duration. The results of kruskal-wallis test indicated that the cytotoxic activity of crude venom extract on cell lines were similar in almost all concentrations and significant differences were not observed (p>0.05). IC50 of crude venom extract against breast (MD-MBA231) and normal (HEK-293) cell lines was observed at 4.13, 117.8µg/ml, respectively. Results obtained from this study showed that increasing the concentration of extract led to the increasing death of both cell lines. Comparing the percentage of death between two cell lines at different concentrations indicated that aqueous extract induced approximately 3.16 folds more cell toxicity on cancer cell line rather than on normal cell lines. Therefore, crude venom extract of <em>S. haddoni</em> can be considered as a strong preventive agent for growth reduction of cancer cell lines especially for breast cancer cell line with low toxicity on normal human cells.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Investigation of some reproductive characteristic of Carasobarbus luteus
in Karoon RiverInvestigation of some reproductive characteristic of Carasobarbus luteus
in Karoon River476011348310.22092/isfj.2017.113483FAM. BiriaN. Javadzadeh ShalkohiA. HosseiniM. VelayatzadehJournal Article20171024<em>Carassobarbus loteus</em> fish, one of the Barbus species and native to southwestern Iran, have been identified in the domestic water in the rivers and wetlands of Khuzestan Province. This study was performed characteristic reproductive biology of <em>Carassobarbus luteus</em> in Karun River. Monthly catches from February 2012 to April 2013 at three stations in Aqili desert- of Shushtar from the Khuzestan Province. 385 samples of <em>Carassobarbus luteus</em> were collected from the study area. In this study, length frequency of fish entire 161.57±23.89 mm and the weight frequency 63.8±35.46 gr. The highest and lowest levels of gonadal index for the month of February (10.629±5.638) and December (0.986±0.560), respectively. In this study was identified 183 males and 180 females and 22 <em>Carassobarbus loteus</em> undetected in Karun River. Average oocyte diameter in sexual stages was II, III, IV and V, 13.818, 15.091, 16.265 and 16.2 mm, respectively. Maximum diameter of fish eggs were observed in February (18.52 mm) and minimum diameter of oocytes in December (13.88 mm). Mean absolute fecundity of <em>Carassobarbus luteus</em> were calculated 5754.59±7 and the average relative fecundity of fishes 53.30±1.03. According to this research has been done upstream spawning season because of differences with other species Barbus can be geographic location length and weight of the fish. Also this species have two peaks of spawning in October and February, so the possibility of being asynchronous spawn.<em>Carassobarbus loteus</em> fish, one of the Barbus species and native to southwestern Iran, have been identified in the domestic water in the rivers and wetlands of Khuzestan Province. This study was performed characteristic reproductive biology of <em>Carassobarbus luteus</em> in Karun River. Monthly catches from February 2012 to April 2013 at three stations in Aqili desert- of Shushtar from the Khuzestan Province. 385 samples of <em>Carassobarbus luteus</em> were collected from the study area. In this study, length frequency of fish entire 161.57±23.89 mm and the weight frequency 63.8±35.46 gr. The highest and lowest levels of gonadal index for the month of February (10.629±5.638) and December (0.986±0.560), respectively. In this study was identified 183 males and 180 females and 22 <em>Carassobarbus loteus</em> undetected in Karun River. Average oocyte diameter in sexual stages was II, III, IV and V, 13.818, 15.091, 16.265 and 16.2 mm, respectively. Maximum diameter of fish eggs were observed in February (18.52 mm) and minimum diameter of oocytes in December (13.88 mm). Mean absolute fecundity of <em>Carassobarbus luteus</em> were calculated 5754.59±7 and the average relative fecundity of fishes 53.30±1.03. According to this research has been done upstream spawning season because of differences with other species Barbus can be geographic location length and weight of the fish. Also this species have two peaks of spawning in October and February, so the possibility of being asynchronous spawn.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Study on catch spatial changes and classifying of beach seine cooperatives in Iranian coast of the Caspian SeaStudy on catch spatial changes and classifying of beach seine cooperatives in Iranian coast of the Caspian Sea616811348410.22092/isfj.2017.113484FAH. FazliGh. DaryanabardJournal Article20171024The objectives of the present study were to determine the structure of capture fisheries and to classify the cooperatives who are involved in beach seine fishery of bony fish, based on the amount of capture fisheries production from 2009 to 2012. In this study, the capture production statistics of 123 beach seine cooperatives located in three provinces of Guilan, Mazandaran and Golestan were collected for each species (<em>Rutilus kutum</em>,<em> Liza</em> spp., <em>Cyprinus carpio</em>,<em> Rutilus rutilus</em>, <em>Vimba vimba</em>,<em> Chalcalburnus chalcoides</em>,<em> Alosa</em> spp., <em>Aspius aspius</em>,<em> Barbus </em>sp., <em>Abramis brama, </em><em>Stizostedion lucioperca </em>and<em> Salmo trutta caspius</em>) and used as input data. According to the results, Caspian kutum represented the highest proportion (66.27%) of total catch among 12 species/groups, followed by mullet, shads and carp with 27.43, 4.26 and 1.32% of total catch, respectively. These 123 beach seine cooperatives were grouped into 3 distinct clusters by performing the complete linkage clustering method and hierarchical cluster analysis. First cluster contained 8 fishing cooperatives. The second and third clusters contained 25 and 90 beach seine cooperatives, respectively. The third cluster had the lowest amount of capture fisheries as compared to the other clusters and these beach seine cooperatives were located in the east and the west parts of the Iranian Caspian coast.The objectives of the present study were to determine the structure of capture fisheries and to classify the cooperatives who are involved in beach seine fishery of bony fish, based on the amount of capture fisheries production from 2009 to 2012. In this study, the capture production statistics of 123 beach seine cooperatives located in three provinces of Guilan, Mazandaran and Golestan were collected for each species (<em>Rutilus kutum</em>,<em> Liza</em> spp., <em>Cyprinus carpio</em>,<em> Rutilus rutilus</em>, <em>Vimba vimba</em>,<em> Chalcalburnus chalcoides</em>,<em> Alosa</em> spp., <em>Aspius aspius</em>,<em> Barbus </em>sp., <em>Abramis brama, </em><em>Stizostedion lucioperca </em>and<em> Salmo trutta caspius</em>) and used as input data. According to the results, Caspian kutum represented the highest proportion (66.27%) of total catch among 12 species/groups, followed by mullet, shads and carp with 27.43, 4.26 and 1.32% of total catch, respectively. These 123 beach seine cooperatives were grouped into 3 distinct clusters by performing the complete linkage clustering method and hierarchical cluster analysis. First cluster contained 8 fishing cooperatives. The second and third clusters contained 25 and 90 beach seine cooperatives, respectively. The third cluster had the lowest amount of capture fisheries as compared to the other clusters and these beach seine cooperatives were located in the east and the west parts of the Iranian Caspian coast.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Monitoring water quality and eutrophication phenomenon of Azad Dam using Iranian Water Quality Index and Carlson's Trophic State IndexMonitoring water quality and eutrophication phenomenon of Azad Dam using Iranian Water Quality Index and Carlson's Trophic State Index697711348510.22092/isfj.2017.113485FAA. MakhlooghH. Nasrollahzadeh SaraviF. ParafkandehH. FazliR. MirzaeiH. HosseinporA. KeyhansaniM. DostdarJournal Article20171024Water reservoirs have an important role in providing water for drinking and agriculture. It is necessary to protect these resources. This study was conducted to determine water quality of Azad Reservoir (Kordestan-Sanandaj) based on Iranian Water Quality Index (IRWQI) and Carlson's Trophic State Index (TSI). Sampling was carried out at five stations during different months in 2015-2016. Results showed that trophic status of Azad dam was mesotrophic based on chlorophyll-<em>a</em> parameters and eutrophic to hypertrophic based on other variables (TP, SDD and DO % at bottom layers). Mean of Carlson's Trophic State Index represented mesotrophic state (high quality) at Azad dam. Based on IRWQI, water quality of dam was classified as excellent quality. Although, mean of indices indicated the suitability of this lake for drinking and agriculture activities but due to declining water quality and increasing trophic level in some months, it is necessary to apply appropriate management strategies for maintaining water quality in the reservoir.Water reservoirs have an important role in providing water for drinking and agriculture. It is necessary to protect these resources. This study was conducted to determine water quality of Azad Reservoir (Kordestan-Sanandaj) based on Iranian Water Quality Index (IRWQI) and Carlson's Trophic State Index (TSI). Sampling was carried out at five stations during different months in 2015-2016. Results showed that trophic status of Azad dam was mesotrophic based on chlorophyll-<em>a</em> parameters and eutrophic to hypertrophic based on other variables (TP, SDD and DO % at bottom layers). Mean of Carlson's Trophic State Index represented mesotrophic state (high quality) at Azad dam. Based on IRWQI, water quality of dam was classified as excellent quality. Although, mean of indices indicated the suitability of this lake for drinking and agriculture activities but due to declining water quality and increasing trophic level in some months, it is necessary to apply appropriate management strategies for maintaining water quality in the reservoir.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Effects of different methods of cooking on mineral and vitamin (A and E) content of yellow fin sea bream (Acanthopagrus latus) fish filletsEffects of different methods of cooking on mineral and vitamin (A and E) content of yellow fin sea bream (Acanthopagrus latus) fish fillets798811348610.22092/isfj.2017.113486FAD. AliporM. Javahery BaboliL. RomianiJournal Article20171024The aim of this study was to investigate the effects of cooking methods including steaming, frying and grilling on the amounts of vitamin (A and E) and mineral (iron, phosphorus, copper, zinc, calcium, magnesium, potassium, and sodium) content of Yellow-fin sea bream (<em>Acanthopagrus latus</em>) fish fillets. This study was performed with four treatments and replicated three times. Results were compared with the amounts of vitamin and mineral contents of raw samples. The vitamin content of samples was measured by the method of high-performance liquid chromatography (HPLC). The mineral content was evaluated by the method of atomic absorption spectrophotometry. The comparison of examined mineral elements in cooked samples with raw samples, showed no significant differences (p>0.05). The vitamin A content of cooked samples was compared with their amounts in raw samples. Results indicated that vitamin A was significantly decreased in cooked samples (p˂0.05). The lowest amounts of vitamin A were observed in fried samples (7.6 ± 0.66 mg/kg of wet weight). The vitamin E content of cooked samples was compared with their amounts in raw samples. Results indicated that vitamin E was significantly decreased in cooked samples (p˂0.05). The lowest levels of vitamin E were observed in grilled samples (0.293 ± 0.02 mg/kg wet weight). These results showed that the mineral content of samples cooked with three methods including steaming, grilling and frying was not significantly different from row samples. Therefore, various methods of cooking can be applied based on the diet. However, the vitamin A and E contents of yellow fin sea bream fish fillets were significantly decreased in cooked samples.The aim of this study was to investigate the effects of cooking methods including steaming, frying and grilling on the amounts of vitamin (A and E) and mineral (iron, phosphorus, copper, zinc, calcium, magnesium, potassium, and sodium) content of Yellow-fin sea bream (<em>Acanthopagrus latus</em>) fish fillets. This study was performed with four treatments and replicated three times. Results were compared with the amounts of vitamin and mineral contents of raw samples. The vitamin content of samples was measured by the method of high-performance liquid chromatography (HPLC). The mineral content was evaluated by the method of atomic absorption spectrophotometry. The comparison of examined mineral elements in cooked samples with raw samples, showed no significant differences (p>0.05). The vitamin A content of cooked samples was compared with their amounts in raw samples. Results indicated that vitamin A was significantly decreased in cooked samples (p˂0.05). The lowest amounts of vitamin A were observed in fried samples (7.6 ± 0.66 mg/kg of wet weight). The vitamin E content of cooked samples was compared with their amounts in raw samples. Results indicated that vitamin E was significantly decreased in cooked samples (p˂0.05). The lowest levels of vitamin E were observed in grilled samples (0.293 ± 0.02 mg/kg wet weight). These results showed that the mineral content of samples cooked with three methods including steaming, grilling and frying was not significantly different from row samples. Therefore, various methods of cooking can be applied based on the diet. However, the vitamin A and E contents of yellow fin sea bream fish fillets were significantly decreased in cooked samples.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Toxicity of some permeable and non-permeable cryoprotectants on common carp (Cyprinus carpio) embryosToxicity of some permeable and non-permeable cryoprotectants on common carp (Cyprinus carpio) embryos899911348710.22092/isfj.2017.113487FAS. KeyvanloM. SodagarM. MazandaraniJournal Article20171024Knowledge of cryoprotectants toxicity is one of the prerequisites for the design of fish embryo cryopreservation protocols. In order to study the effects of cryoprotectants toxicity, common carp (<em>Cyprinus carpio</em>) embryos at half-epiboly and heartbeat stages were selected and exposed to pronase E, type XIV of <em>Streptomyces griseus</em>, (2 mg/ml in Ringer solution) for 5 min. Permeabilized embryos were immersed in: three permeable cryoprotectants, dimethyl sulfoxide (DMSO), methanol (MeOH) and propylene glycol (PG) in concentrations ranging from 1 to 4 M; and one non-permeable cryoprotectant, sucrose (in concentrations of 10%, 15% and 20%) for 5 and 15 min. After these treatments, the embryos were washed and incubated until hatched. The toxicity of the cryoprotectant was assessed by the hatching rate. The results showed that there was a significant decrease of hatching rate in both developmental stages with increased concentration and duration of exposure. In addition, MeOH was the least toxic permeable cryoprotectant, followed by PG and DMSO. Prolonged exposure to sucrose significantly reduced hatching rate, especially at the heartbeat stage. With the increasing of the embryonic development (from half-epiboly to heartbeat stage) sensitivity to cryoprotectants were decreased.Knowledge of cryoprotectants toxicity is one of the prerequisites for the design of fish embryo cryopreservation protocols. In order to study the effects of cryoprotectants toxicity, common carp (<em>Cyprinus carpio</em>) embryos at half-epiboly and heartbeat stages were selected and exposed to pronase E, type XIV of <em>Streptomyces griseus</em>, (2 mg/ml in Ringer solution) for 5 min. Permeabilized embryos were immersed in: three permeable cryoprotectants, dimethyl sulfoxide (DMSO), methanol (MeOH) and propylene glycol (PG) in concentrations ranging from 1 to 4 M; and one non-permeable cryoprotectant, sucrose (in concentrations of 10%, 15% and 20%) for 5 and 15 min. After these treatments, the embryos were washed and incubated until hatched. The toxicity of the cryoprotectant was assessed by the hatching rate. The results showed that there was a significant decrease of hatching rate in both developmental stages with increased concentration and duration of exposure. In addition, MeOH was the least toxic permeable cryoprotectant, followed by PG and DMSO. Prolonged exposure to sucrose significantly reduced hatching rate, especially at the heartbeat stage. With the increasing of the embryonic development (from half-epiboly to heartbeat stage) sensitivity to cryoprotectants were decreased.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Optimizing the chemical composition of fish fillets and blood biochemical parameters of common carp (Cyprinus carpio) using various levels of commercial probiotic Lactobacillus (Lactobacillus acidophilus) in the dietOptimizing the chemical composition of fish fillets and blood biochemical parameters of common carp (Cyprinus carpio) using various levels of commercial probiotic Lactobacillus (Lactobacillus acidophilus) in the diet10111011348810.22092/isfj.2017.113488FAF. BaesiA. AberomandS. ZiaeinezhadM. Javaheri BaboliJournal Article20171024The effects of using commercial probiotic <em>Lactobacillus</em> in the diet of common carp (<em>Cyprinus carpio</em>) on the chemical composition of fishfillets and blood biochemical parameters were studied in this investigation.In this order, 243 fish fed the commercial probiotic <em>Lactobacillus</em> diet with two treatments including the concentration of zero (control group, C), 10<sup>3 </sup>CFUg<sup>-1</sup> (treatment A) and 10<sup>6 </sup>CFUg<sup>-1</sup> (treatment B) of this probiotic.The results showed that the fat content of fish fillets was the highest in group B and lowest in group A, whereas the results of both these treatments were significantly different from group C (p<0.05).Fish in the group C had the lowest amounts of ash as compared to the fish fed with probiotics. The highest and the lowest amounts of moisture were seen in group A and B, respectively. The moisture content of both these treatments were significantly different from group C (p<0.05). Group A showed the highest amount of protein which was not significantly different from other groups (p>0.05). The highest and lowest amounts of cholesterol and triglycerides were seen in group B and A, respectively. Serum total protein decreased during the experiment, whereas the amount of albumin increased. Accordingly, this investigation recommends the use of 10<sup>6 </sup>CFUg<sup>-1</sup> (treatment B) of probiotic <em>Lactobacillus</em> in fish diet to improve the nutritional value of common carp.The effects of using commercial probiotic <em>Lactobacillus</em> in the diet of common carp (<em>Cyprinus carpio</em>) on the chemical composition of fishfillets and blood biochemical parameters were studied in this investigation.In this order, 243 fish fed the commercial probiotic <em>Lactobacillus</em> diet with two treatments including the concentration of zero (control group, C), 10<sup>3 </sup>CFUg<sup>-1</sup> (treatment A) and 10<sup>6 </sup>CFUg<sup>-1</sup> (treatment B) of this probiotic.The results showed that the fat content of fish fillets was the highest in group B and lowest in group A, whereas the results of both these treatments were significantly different from group C (p<0.05).Fish in the group C had the lowest amounts of ash as compared to the fish fed with probiotics. The highest and the lowest amounts of moisture were seen in group A and B, respectively. The moisture content of both these treatments were significantly different from group C (p<0.05). Group A showed the highest amount of protein which was not significantly different from other groups (p>0.05). The highest and lowest amounts of cholesterol and triglycerides were seen in group B and A, respectively. Serum total protein decreased during the experiment, whereas the amount of albumin increased. Accordingly, this investigation recommends the use of 10<sup>6 </sup>CFUg<sup>-1</sup> (treatment B) of probiotic <em>Lactobacillus</em> in fish diet to improve the nutritional value of common carp.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Effects of different protein restriction and realimentation on growth performance and body composition of fingerling Common Carp (CyprinusCarpio)Effects of different protein restriction and realimentation on growth performance and body composition of fingerling Common Carp (CyprinusCarpio)11112011348910.22092/isfj.2017.113489FAH. SancholiH. OrojiA. Keramat AmirkolaiiKh. Jani KhaliliJournal Article20171024In this study, the effects of protein restriction in the diet and re-feeding with complete diet on growth performance and body composition of fingerling common carp <em>(Cyprinus carpio)</em> were studied during 8 weeks. Common carp fingerlings with an average initial weight (20/09 ± 0/07) in seven experimental groups were fed with three replications. Treatment A: 8 weeks were fed diet with 32% protein (control group), for treatments of B, C, D, E, F and G respectively the protein restriction phase was considered one week, two weeks, three weeks, four weeks, one week within three weeks of feeding the diet containing 16% protein as dietary protein restriction Highest final weight, body weight increase, percentage of body weight gain, feed conversion efficiency and specific growth rate was observed in group C and the lowest was observed in group E. Highest feed conversion ratio was observed in treatment C which significantly higher than treatment D and E (p<0/05). Highest protein efficiency ratio was observed in treatment F which showed no significant difference between treatments C and G (p>0.05) but with other treatments were significantly different (p<0/05).none of the treatments ash did not show a significant difference together (p>0/05). Highest of the moisture content of the carcass observed in treatment E which not significantly different with treatments F and G (p>0/05) but showed significant differences (p<0/05) with other treatments. Also highest carcass protein and fat was levels in treatment D and F were observed. In the end it can be concluded that the more protein during long periods of limitation, the ability of fish to compensate for the slowdown, but will be less than the length of the limitation period is less protein, the better fish can compensate for the lack.In this study, the effects of protein restriction in the diet and re-feeding with complete diet on growth performance and body composition of fingerling common carp <em>(Cyprinus carpio)</em> were studied during 8 weeks. Common carp fingerlings with an average initial weight (20/09 ± 0/07) in seven experimental groups were fed with three replications. Treatment A: 8 weeks were fed diet with 32% protein (control group), for treatments of B, C, D, E, F and G respectively the protein restriction phase was considered one week, two weeks, three weeks, four weeks, one week within three weeks of feeding the diet containing 16% protein as dietary protein restriction Highest final weight, body weight increase, percentage of body weight gain, feed conversion efficiency and specific growth rate was observed in group C and the lowest was observed in group E. Highest feed conversion ratio was observed in treatment C which significantly higher than treatment D and E (p<0/05). Highest protein efficiency ratio was observed in treatment F which showed no significant difference between treatments C and G (p>0.05) but with other treatments were significantly different (p<0/05).none of the treatments ash did not show a significant difference together (p>0/05). Highest of the moisture content of the carcass observed in treatment E which not significantly different with treatments F and G (p>0/05) but showed significant differences (p<0/05) with other treatments. Also highest carcass protein and fat was levels in treatment D and F were observed. In the end it can be concluded that the more protein during long periods of limitation, the ability of fish to compensate for the slowdown, but will be less than the length of the limitation period is less protein, the better fish can compensate for the lack.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Study on primary production with emphasis on phytoplankton biovolume and chlorophyll-a in the southern Caspian Sea-Goharbaran regionStudy on primary production with emphasis on phytoplankton biovolume and chlorophyll-a in the southern Caspian Sea-Goharbaran region12113011349010.22092/isfj.2017.113490FAA. MakhloghH. Narollahzadeh SaraviF. EslamiA. KeyhansaniF. VahediJournal Article20171024Phytoplankton biovolume and concentration of chlorophyll <em>a</em> are of major concerns in estimating the primary production in aquatic ecosystems. The objective of the present study was to estimate the concentration of chlorophyll <em>a</em> and its relation to variations of some phytoplankton community structure parameters and abiotic factors (environment and nutrients) in the coastal ecosystem of the Caspian Sea in Goharbaran region. Samples were collected monthly from various layers of water (surface, 5 and 10 m) in three depths (5, 10 and 15 m) during 2013-2014. Based on the results the minimum and maximum mean values (±SE) of biomass were obtained in summer (94± 16) and winter (1209± 106) mg/m<sup>3</sup>, respectively. The minimum and maximum mean values (±SE) of chlorophyll <em>a</em> were recorded in spring (0.39±0.05 µg/L) and autumn (4.56± 0.23 µg/L), respectively. Seasonal changes in the pattern of dominant taxonomic groups of phytoplankton showed important role in relationship between chlorophyll content of the cells and abiotic factors. Temperature (in summer and autumn), phosphorous (in spring), nitrogen (in summer) and silicon (in spring and winter) showed significant effects on incompatibility between phytoplankton biovolume and the concentration of chlorophyll. As conclusion, difference between the patterns of change in chlorophyll and phytoplankton biovolume indicated the importance of choosing appropriate parameters in order to estimate the primary production and to predict the algal bloom, especially in the cage and pen culture sites. Since the rapid changes in the chlorophyll-biovolume relationship could lead to significant errors in the explanation of results and unexpected field observations.Phytoplankton biovolume and concentration of chlorophyll <em>a</em> are of major concerns in estimating the primary production in aquatic ecosystems. The objective of the present study was to estimate the concentration of chlorophyll <em>a</em> and its relation to variations of some phytoplankton community structure parameters and abiotic factors (environment and nutrients) in the coastal ecosystem of the Caspian Sea in Goharbaran region. Samples were collected monthly from various layers of water (surface, 5 and 10 m) in three depths (5, 10 and 15 m) during 2013-2014. Based on the results the minimum and maximum mean values (±SE) of biomass were obtained in summer (94± 16) and winter (1209± 106) mg/m<sup>3</sup>, respectively. The minimum and maximum mean values (±SE) of chlorophyll <em>a</em> were recorded in spring (0.39±0.05 µg/L) and autumn (4.56± 0.23 µg/L), respectively. Seasonal changes in the pattern of dominant taxonomic groups of phytoplankton showed important role in relationship between chlorophyll content of the cells and abiotic factors. Temperature (in summer and autumn), phosphorous (in spring), nitrogen (in summer) and silicon (in spring and winter) showed significant effects on incompatibility between phytoplankton biovolume and the concentration of chlorophyll. As conclusion, difference between the patterns of change in chlorophyll and phytoplankton biovolume indicated the importance of choosing appropriate parameters in order to estimate the primary production and to predict the algal bloom, especially in the cage and pen culture sites. Since the rapid changes in the chlorophyll-biovolume relationship could lead to significant errors in the explanation of results and unexpected field observations.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Histopathological study as bio-indicators for evaluation of water quality in rainbow trout fish farms along the Baram Springs of LordeganHistopathological study as bio-indicators for evaluation of water quality in rainbow trout fish farms along the Baram Springs of Lordegan13113811349110.22092/isfj.2017.113491FAS. MahmodiM. BanaeiP. ShokatA. NoriL. Mosavi DehmoorediJournal Article20171024The purpose of this study was to evaluate histopathological changes in gills, spleen, liver, kidney and intestine of farmed rainbow trout in four different stations along the Barm Springs (Lordegan, Iran). In this study, at least 15 fish from each farm were captured and following autopsy, sampling was carried out from the gills, liver, spleen and intestine of fish. After tissue sectioning, samples were stained with hematoxylin and eosin. Tissues were studied at 400X magnification. Gill lamellae that sticking together, gill hyperplasia, exfoliation of gill epithelium, epithelial necrosis and hypertrophy and swelling of gill lamellae, hypertrophy and liver lipoid degeneration, glomerular degeneration, degeneration of collecting duct of urinary system, increasing volume of the lumen space, shrinking of glomerular size, increasing in melanomacrophage centers, disorganization of ellipsoids in the spleen, necrosis of epithelial cells and exfoliation of the mucosal layer of intestine were the major histopathological alterations which were observed in the fourth (less than 20% of the group) and the third (a few of fish) group. The results of this study showed that these alterations may be due to increasing amounts of chemicals in downstream water. Therefore, these histopathological changes can be used as appropriate biomarkers for assessing water quality.The purpose of this study was to evaluate histopathological changes in gills, spleen, liver, kidney and intestine of farmed rainbow trout in four different stations along the Barm Springs (Lordegan, Iran). In this study, at least 15 fish from each farm were captured and following autopsy, sampling was carried out from the gills, liver, spleen and intestine of fish. After tissue sectioning, samples were stained with hematoxylin and eosin. Tissues were studied at 400X magnification. Gill lamellae that sticking together, gill hyperplasia, exfoliation of gill epithelium, epithelial necrosis and hypertrophy and swelling of gill lamellae, hypertrophy and liver lipoid degeneration, glomerular degeneration, degeneration of collecting duct of urinary system, increasing volume of the lumen space, shrinking of glomerular size, increasing in melanomacrophage centers, disorganization of ellipsoids in the spleen, necrosis of epithelial cells and exfoliation of the mucosal layer of intestine were the major histopathological alterations which were observed in the fourth (less than 20% of the group) and the third (a few of fish) group. The results of this study showed that these alterations may be due to increasing amounts of chemicals in downstream water. Therefore, these histopathological changes can be used as appropriate biomarkers for assessing water quality.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Effects of arginine, phenylalanine and tryptophan deficiency in diets on growth factors and whole body proximate of Sobaity seabream juvenile (Sparidentex hasta)Effects of arginine, phenylalanine and tryptophan deficiency in diets on growth factors and whole body proximate of Sobaity seabream juvenile (Sparidentex hasta)13915111349210.22092/isfj.2017.113492FAJ. Ghafle MarammaziM. YaghobiO. SafariJournal Article20171024On this study effects of reducing arginine, phenylalanine and tryptophan amino acids in diets of Sobaity seabream in a constant level (40%) on growth, nutritional indices, whole body chemical proximate and amino acids, was assessed. For these purposes four different treatments in triplicate was used during 42 days in base of feeding with semipurified diets containing crystalline amino acids. The control diet was without any deficiency, the arginine (ARG), phenylalanine (PHE) and tryptophan (TRP) diets were deficient in arginine, phenylalanine and tryptophan respectively. Crystalline amino acids were used to formulate diets precisely with desired amounts of deficiency. In fish fed ARG diet, just two factors including nitrogen retention and percentage of weight gain decreased in comparing with control group (p> 0.05). But in PHE and TRP treatments all the growth and nutrition factors including final weight, weight gain, specific growth rate, feed consumption, protein efficiency and nitrogen retention decreased significantly regarding to the control group (p>0.05). The experimental treatments affect whole body proximate regarding to the control by increasing whole body moisture in PHE, reduction of gross energy in all treatments and reduction of Crude lipids in TRP and PHE (p>0.05). Reduction of whole body protein in ARG treatment showed using more protein rather than lipids as a source of energy in this group of fish. Reduction of whole body lipid in all treatments regarding to the control group showed some disorder in lipid metabolism. In spite of arginine, phenylalanine and tryptophan deficiency in diets, the amounts of these amino acids in fish whole body did not decreased that showed amino acids sparing effect for these three essential amino acids happened in fish body. The results of the current study showed that reduction of one single essential amino acid from diet have strong effects on other amino acids retention and consequently reduces protein synthesis and growth.On this study effects of reducing arginine, phenylalanine and tryptophan amino acids in diets of Sobaity seabream in a constant level (40%) on growth, nutritional indices, whole body chemical proximate and amino acids, was assessed. For these purposes four different treatments in triplicate was used during 42 days in base of feeding with semipurified diets containing crystalline amino acids. The control diet was without any deficiency, the arginine (ARG), phenylalanine (PHE) and tryptophan (TRP) diets were deficient in arginine, phenylalanine and tryptophan respectively. Crystalline amino acids were used to formulate diets precisely with desired amounts of deficiency. In fish fed ARG diet, just two factors including nitrogen retention and percentage of weight gain decreased in comparing with control group (p> 0.05). But in PHE and TRP treatments all the growth and nutrition factors including final weight, weight gain, specific growth rate, feed consumption, protein efficiency and nitrogen retention decreased significantly regarding to the control group (p>0.05). The experimental treatments affect whole body proximate regarding to the control by increasing whole body moisture in PHE, reduction of gross energy in all treatments and reduction of Crude lipids in TRP and PHE (p>0.05). Reduction of whole body protein in ARG treatment showed using more protein rather than lipids as a source of energy in this group of fish. Reduction of whole body lipid in all treatments regarding to the control group showed some disorder in lipid metabolism. In spite of arginine, phenylalanine and tryptophan deficiency in diets, the amounts of these amino acids in fish whole body did not decreased that showed amino acids sparing effect for these three essential amino acids happened in fish body. The results of the current study showed that reduction of one single essential amino acid from diet have strong effects on other amino acids retention and consequently reduces protein synthesis and growth.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Comparative study of the growth of microalgae Chlorella sp. in marine water and non-sterile wastewaterComparative study of the growth of microalgae Chlorella sp. in marine water and non-sterile wastewater15316211349310.22092/isfj.2017.113493FASh. BagheriZ. MasomizadehJournal Article20171024<em>Chlorella</em> is important green algae that can be used for bioremediation of wastewaters. It can highly tolerate adverse environmental condition. Wastewaters contain high levels of nutrients which can be used as appropriate mediums for algal culture. In this study the effects of using vitamins on the growth of microalgae <em>Chlorella </em>sp. were investigated in marine water and wastewater using various mediums including Guillard, Conway, TMRL and B complex medium. Culture mediums with seawater and wastewater had 5 and 6 different treatments, respectively. Each treatment was replicated tree times. According to the investigations it was found that there are significant differences in <em>Chlorella</em> growth in sterilized seawater, between the medium with Gillard vitamins and Conway vitamins and medium without vitamin and also between Conway medium and other treatments, including culture mediums containing vitamin compounds and no vitamins, while are not significant between the treatments with vitamin compounds and as well as between B vitamins medium and medium without vitamin in seawater. Significant difference was not found between treatments in non-sterile wastewater (p>0/05). Therefore, adding vitamin compounds or using Conway medium did not lead to a significant increase in the growth of <em>Chlorella</em> sp. in non-sterile wastewater.<em>Chlorella</em> is important green algae that can be used for bioremediation of wastewaters. It can highly tolerate adverse environmental condition. Wastewaters contain high levels of nutrients which can be used as appropriate mediums for algal culture. In this study the effects of using vitamins on the growth of microalgae <em>Chlorella </em>sp. were investigated in marine water and wastewater using various mediums including Guillard, Conway, TMRL and B complex medium. Culture mediums with seawater and wastewater had 5 and 6 different treatments, respectively. Each treatment was replicated tree times. According to the investigations it was found that there are significant differences in <em>Chlorella</em> growth in sterilized seawater, between the medium with Gillard vitamins and Conway vitamins and medium without vitamin and also between Conway medium and other treatments, including culture mediums containing vitamin compounds and no vitamins, while are not significant between the treatments with vitamin compounds and as well as between B vitamins medium and medium without vitamin in seawater. Significant difference was not found between treatments in non-sterile wastewater (p>0/05). Therefore, adding vitamin compounds or using Conway medium did not lead to a significant increase in the growth of <em>Chlorella</em> sp. in non-sterile wastewater.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Efficiency of sewage treatment lagoon on pollution load reduction of Gomishan shrimp farm effluent in Golestan ProvinceEfficiency of sewage treatment lagoon on pollution load reduction of Gomishan shrimp farm effluent in Golestan Province16316711349410.22092/isfj.2017.113494FAA. KhodashenasA. HedayatiR. GhorbaniA. HosseiniM. SaghaliJournal Article20171024This research was conducted on shrimp farms complex in the northern of Gomishan city, Golestan province. In order to reduce water pollution, around 30 hectares of sedimentation lagoon was constructed on the way of the main outflow channel of shrimp farm. For this project, three sampling stations were considered at the entrance of the shrimp farm, entrance of the lagoon (before entering the lagoon) and way out of the lagoon during the last 3 months of shrimp farming (at the end of August, September and October 2015). Physicochemical parameters including EC, BOD, NO<sub>3</sub> and PO<sub>4</sub> were measured. The amounts of BOD, nitrate and phosphate ions were not significantly different (p>0.05) at three sampling stations which indicated that the impact of shrimp farming on these factors was not severe. The amounts of these factors at the entrance of the farm were the lowest and at the entrance of the lagoon were the highest. The amounts of these parameters were reduced in lagoon outflow. However, all of these changes were not significantly different from each other (p>0.05). In general, Gomishan shrimp farming with this volume of production and the 30 hectares lagoon is not a serious threat for Gomishan wetland and the Caspian Sea ecosystem. But if the area of shrimp farms increases, some solutions should be found for wastewater treatment because of the increasing possibility of wetland pollution.This research was conducted on shrimp farms complex in the northern of Gomishan city, Golestan province. In order to reduce water pollution, around 30 hectares of sedimentation lagoon was constructed on the way of the main outflow channel of shrimp farm. For this project, three sampling stations were considered at the entrance of the shrimp farm, entrance of the lagoon (before entering the lagoon) and way out of the lagoon during the last 3 months of shrimp farming (at the end of August, September and October 2015). Physicochemical parameters including EC, BOD, NO<sub>3</sub> and PO<sub>4</sub> were measured. The amounts of BOD, nitrate and phosphate ions were not significantly different (p>0.05) at three sampling stations which indicated that the impact of shrimp farming on these factors was not severe. The amounts of these factors at the entrance of the farm were the lowest and at the entrance of the lagoon were the highest. The amounts of these parameters were reduced in lagoon outflow. However, all of these changes were not significantly different from each other (p>0.05). In general, Gomishan shrimp farming with this volume of production and the 30 hectares lagoon is not a serious threat for Gomishan wetland and the Caspian Sea ecosystem. But if the area of shrimp farms increases, some solutions should be found for wastewater treatment because of the increasing possibility of wetland pollution.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426220170622Investigating the effect of Caraway (Carum carvi) on some growth indexes and body approximate composition of Beluga, Huso huso (Linnaeus; 1758) fingerlingsInvestigating the effect of Caraway (Carum carvi) on some growth indexes and body approximate composition of Beluga, Huso huso (Linnaeus; 1758) fingerlings16917211349510.22092/isfj.2017.113495FAM. YasamiA. SarpanahM. MahmoodiM. ParsaJournal Article20171024In this study, in order to investigate the effect of Caraway (<em>Carum carvi</em>) on growth parameters and body approximate composition of <em>Huso huso</em> (Linnaeus; 1758) fingerlings, 3 diet containing 10%, 15% and 20% of Caraway with control diet that had not Caraway in its composition was utilized for feeding the <em>Huso huso</em> fingerlings. Fishes with initial mean weight of 10 g were fed with control diets in 7 weeks and growth parameters including total length, total weight, protein efficiency ratio, feed conversation rate and body approximate compositions were measured and analyzed. Results showed that the diet with 15% Caraway has the best effect on growth parameters of <em>Huso huso</em> fingerlings and lead to more gaining weight (146.60±1.85 g) than other diets. Moreover, between body approximate compositions, only the protein and moisture level were affected by diets containing Caraway. Maximum protein was observed in fingerlings fed with diets containing 10% and 20% Caraway. According to obtained results, using the diet with 15% Caraway recommend in order to enhance growth of <em>Huso huso</em> fingerlings.In this study, in order to investigate the effect of Caraway (<em>Carum carvi</em>) on growth parameters and body approximate composition of <em>Huso huso</em> (Linnaeus; 1758) fingerlings, 3 diet containing 10%, 15% and 20% of Caraway with control diet that had not Caraway in its composition was utilized for feeding the <em>Huso huso</em> fingerlings. Fishes with initial mean weight of 10 g were fed with control diets in 7 weeks and growth parameters including total length, total weight, protein efficiency ratio, feed conversation rate and body approximate compositions were measured and analyzed. Results showed that the diet with 15% Caraway has the best effect on growth parameters of <em>Huso huso</em> fingerlings and lead to more gaining weight (146.60±1.85 g) than other diets. Moreover, between body approximate compositions, only the protein and moisture level were affected by diets containing Caraway. Maximum protein was observed in fingerlings fed with diets containing 10% and 20% Caraway. According to obtained results, using the diet with 15% Caraway recommend in order to enhance growth of <em>Huso huso</em> fingerlings.