Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Phenotypic and genotypic evaluation of resistance to broad –range antimicrobial drugs in Gram- positive cocci isolated from rainbow trout in IlamPhenotypic and genotypic evaluation of resistance to broad –range antimicrobial drugs in Gram- positive cocci isolated from rainbow trout in Ilam11011351710.22092/isfj.2017.113517FAA YariM NematiF PourahmadJournal Article20171105Streptococci and lactococci are among bacterial diseases of wild and farmed fish especially rainbow trout and accounted for significant economical losses in the aquaculture industry. In this study, 60 fish from rainbow trout farms in Ilam, were collected and sampled for bacterial and molecular analyses. Identification of the isolated bacteria was determined using biochemical tests and confirmed by polymerase chain reaction (PCR). For this purpose, using primer sets targeting portions of the <em>tuf</em> (code along factor Tu) and 16S rRNA gene sequences were performed to determine the genus <em>Streptococcus</em> and <em>Streptococcus</em> <em>iniae</em>, respectively. In total, 110 isolates belonging to the genus <em>Streptococcus</em>, <em>Lactococcus</em> and <em>Staphylococcus </em>were detected. Molecular tests showed that 90 isolates were <em>Streptococcus </em>genus, of which 35 isolates tested by species-specific primers were identified as <em>Streptococcus</em> <em>iniae</em> and the rest (55 isolates) belonged to the species <em>Lactococcus garvieae</em>. Subsequently, phenotypic and genotypic evaluations of antibiotic-resistance of the isolates were performed, using disk diffusion method and PCR targeting <em>tet</em>, <em>erm</em> and <em>str</em> genes. The results showed that of the 110 isolates of Gram-positive cocci, 39 isolates were resistant against streptomycin (35%), 26 to erythromycin (23%), 35 to oxytetracycline (31%), 30 to enrofloxacin (27%), 21 to florfenicol (19%) and 33 isolates were resistant to penicillin G (30%). The presence of streptococcosis / lactococcosis was confirmed among fish farms in Ilam within this study. Moreover, it seems that the indiscriminate use of antibiotics has led to stablish resistant against such infectious agents. <br /><strong> </strong>Streptococci and lactococci are among bacterial diseases of wild and farmed fish especially rainbow trout and accounted for significant economical losses in the aquaculture industry. In this study, 60 fish from rainbow trout farms in Ilam, were collected and sampled for bacterial and molecular analyses. Identification of the isolated bacteria was determined using biochemical tests and confirmed by polymerase chain reaction (PCR). For this purpose, using primer sets targeting portions of the <em>tuf</em> (code along factor Tu) and 16S rRNA gene sequences were performed to determine the genus <em>Streptococcus</em> and <em>Streptococcus</em> <em>iniae</em>, respectively. In total, 110 isolates belonging to the genus <em>Streptococcus</em>, <em>Lactococcus</em> and <em>Staphylococcus </em>were detected. Molecular tests showed that 90 isolates were <em>Streptococcus </em>genus, of which 35 isolates tested by species-specific primers were identified as <em>Streptococcus</em> <em>iniae</em> and the rest (55 isolates) belonged to the species <em>Lactococcus garvieae</em>. Subsequently, phenotypic and genotypic evaluations of antibiotic-resistance of the isolates were performed, using disk diffusion method and PCR targeting <em>tet</em>, <em>erm</em> and <em>str</em> genes. The results showed that of the 110 isolates of Gram-positive cocci, 39 isolates were resistant against streptomycin (35%), 26 to erythromycin (23%), 35 to oxytetracycline (31%), 30 to enrofloxacin (27%), 21 to florfenicol (19%) and 33 isolates were resistant to penicillin G (30%). The presence of streptococcosis / lactococcosis was confirmed among fish farms in Ilam within this study. Moreover, it seems that the indiscriminate use of antibiotics has led to stablish resistant against such infectious agents. <br /><strong> </strong>Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Use of chemical method for preparing of protein hydrolysate from Silver carp (Hypophthalmichthys moltirix) viscera and its application as culture media for Listeria monocytogenesUse of chemical method for preparing of protein hydrolysate from Silver carp (Hypophthalmichthys moltirix) viscera and its application as culture media for Listeria monocytogenes112211351810.22092/isfj.2017.113518FAM AsgharniaS YeganehS.A JafarpourR SafariJournal Article20171105The aim of this study is to chemically hydrolyze of silver carp (<em>Hypophthalmichthys moltirix</em>) viscera and the effect of obtained peptones as a nitrogen source for culture of <em>Listeria monocytogenes</em>. Silver carp viscera were provided by a local market in Sari, Northern Iran. After freeze-thawing of Viscera at 4°C was used for hydrolysis. Acidic and alkaline hydrolysis was done at two pH 3.3 and 12, and two temperatures of 70 and 85°C. At the end of hydrolysis DH% (hydrolysis degree) and protein content were measured. Then obtained peptones from these 4 treatments (three replicates for each treatment) used as <em>Listeria monocytogenes</em> culture media at 48 hours and compared with BHI culture media. Results showed that maximum and minimum protein concentration were related to alkaline hydrolysis at 85 °C and acidic hydrolysis at 70 °C, respectively (p<0.05). The highest DH% was related to alkaline hydrolysis at 85 °C and the lowest of it was related to alkaline hydrolysis at 70 °C (p<0.05). Growth of <em>Listeria monocytogenes</em> in acidic and alkaline produced treatments at 70 °C had no significant differences compared to control (BHI) except at 36 and 48h. But at 85 °C had differences at all hours significantly (p<0.05). This study showed that alkaline hydrolysis in higher temperature is better than acidic hydrolysis and growth of bacteria in fish peptone could be done as well as commercial cultured media. Due to usage of fish viscera and alkaline method with low processing cost, it‘s economically suitable.The aim of this study is to chemically hydrolyze of silver carp (<em>Hypophthalmichthys moltirix</em>) viscera and the effect of obtained peptones as a nitrogen source for culture of <em>Listeria monocytogenes</em>. Silver carp viscera were provided by a local market in Sari, Northern Iran. After freeze-thawing of Viscera at 4°C was used for hydrolysis. Acidic and alkaline hydrolysis was done at two pH 3.3 and 12, and two temperatures of 70 and 85°C. At the end of hydrolysis DH% (hydrolysis degree) and protein content were measured. Then obtained peptones from these 4 treatments (three replicates for each treatment) used as <em>Listeria monocytogenes</em> culture media at 48 hours and compared with BHI culture media. Results showed that maximum and minimum protein concentration were related to alkaline hydrolysis at 85 °C and acidic hydrolysis at 70 °C, respectively (p<0.05). The highest DH% was related to alkaline hydrolysis at 85 °C and the lowest of it was related to alkaline hydrolysis at 70 °C (p<0.05). Growth of <em>Listeria monocytogenes</em> in acidic and alkaline produced treatments at 70 °C had no significant differences compared to control (BHI) except at 36 and 48h. But at 85 °C had differences at all hours significantly (p<0.05). This study showed that alkaline hydrolysis in higher temperature is better than acidic hydrolysis and growth of bacteria in fish peptone could be done as well as commercial cultured media. Due to usage of fish viscera and alkaline method with low processing cost, it‘s economically suitable.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320180220Assessment of productivity of warm water fish production factors in Kermanshah province farmsAssessment of productivity of warm water fish production factors in Kermanshah province farms293811785010.22092/isfj.2018.117850FAA. NajafiS. DelangizanM. AlmasiK. GharraJournal Article20181204Fish is one of the domestic products that is unmatched in terms of protein and unsaturated fat, and its importance as a healthy food is known to all. Keeping in mind, resource scarcity, economic method of increasing this product is the optimum use of the factors of production. The aim of this research was to estimate production function and calculation of productivity of warm water fish production factors in Kermanshah province. The statistical population in this reaserch were all of active, fish farms which were studied in full details. A part of required data of this research was collected from official statistic books of government and another part from interview with farmers and experts of fishery department of Kermanshah through distribution of 46 questionnaires. The production function has been estimated with help of Eviews and with OLS approach and through priority model of Cobb-Douglas was finally selected. Result showed that area under cultivation (land), concentrate and maize, fodder, lime stone, labour, experience of manager of the farm, use of air purifier machine and geographical condition of the region had significant effect on production (p<0.05). Total coefficient of independent variable in estimated production function was approximately 1.1 which was an indication of increasing return to scale. Elasticity of production of area under cultivation was more than other inputs which indicated that the area under cultivation must expand and more area go under cultivation of this product. In addition coefficient of labour was estimated 0.31 which indicated more employment of labour force must be done in order to approach towards optimum product. According to obtained results of this research, education did not have a significant effect on production, while experience had a positive and significant effect on it and this showed that the level of technology in this industry was not so high. The finding also showed that geographical region and use of air purifier had a significant effect on the production of warm water fish. Therefore on this base the farms under review were divided into three categories and the average productivity, marginal productivity and total factors productivity were calculated for each one of these categories. Just in small number of farms, related to one of the categories the measure of VMP<sub>xi </sub>/ P<sub>xi</sub> for descriptive variable was equal to 1 and for other two categories this measure was not equal to one. Which means that the inputs were underemployed? Therefor it seems in order to improve efficiency in input consumption, the fish farm owner can attain maximum profit by using substitution principle.Fish is one of the domestic products that is unmatched in terms of protein and unsaturated fat, and its importance as a healthy food is known to all. Keeping in mind, resource scarcity, economic method of increasing this product is the optimum use of the factors of production. The aim of this research was to estimate production function and calculation of productivity of warm water fish production factors in Kermanshah province. The statistical population in this reaserch were all of active, fish farms which were studied in full details. A part of required data of this research was collected from official statistic books of government and another part from interview with farmers and experts of fishery department of Kermanshah through distribution of 46 questionnaires. The production function has been estimated with help of Eviews and with OLS approach and through priority model of Cobb-Douglas was finally selected. Result showed that area under cultivation (land), concentrate and maize, fodder, lime stone, labour, experience of manager of the farm, use of air purifier machine and geographical condition of the region had significant effect on production (p<0.05). Total coefficient of independent variable in estimated production function was approximately 1.1 which was an indication of increasing return to scale. Elasticity of production of area under cultivation was more than other inputs which indicated that the area under cultivation must expand and more area go under cultivation of this product. In addition coefficient of labour was estimated 0.31 which indicated more employment of labour force must be done in order to approach towards optimum product. According to obtained results of this research, education did not have a significant effect on production, while experience had a positive and significant effect on it and this showed that the level of technology in this industry was not so high. The finding also showed that geographical region and use of air purifier had a significant effect on the production of warm water fish. Therefore on this base the farms under review were divided into three categories and the average productivity, marginal productivity and total factors productivity were calculated for each one of these categories. Just in small number of farms, related to one of the categories the measure of VMP<sub>xi </sub>/ P<sub>xi</sub> for descriptive variable was equal to 1 and for other two categories this measure was not equal to one. Which means that the inputs were underemployed? Therefor it seems in order to improve efficiency in input consumption, the fish farm owner can attain maximum profit by using substitution principle.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823The effect of dietary supplement spirulina (Arthrospira platensis) on the immune system and blood biochemical factors of koi fish (Cyprinus carpio carpio)The effect of dietary supplement spirulina (Arthrospira platensis) on the immune system and blood biochemical factors of koi fish (Cyprinus carpio carpio)233211351910.22092/isfj.2017.113519FAF AnsarifardH Rajabi IslamiM Shamsaie MehrjanM SoltaniJournal Article20171105This study evaluated the effect of diets containing 0, 2.5, 5, 7.5 and 10% <em>Arthrospir platensis </em>on the hematological and immune parametersof Koi carp (<em>Cyprinus carpio</em>). A completely randomized experimental design was developed with five treatments and three replicates for 8 weeks. At the end of experiment, the hematological and, immune system parameters were measured and analyzed. The results revealed that supplementation of <em>Arthrospira platensis</em> in diets results in higher percentage of Hb, Hct, RBC and WBC. In addition, a significant increasein the levels of IgM, lysozyme and C4 complement levels were found in fish fed with 10% <em>Arthrospira platensis </em>(p<0.05). These results revealed that, inclusion of 10% <em>A. platensis</em> in diets did appear to have a significant positive effect on stimulation of immune system of Koi fish.This study evaluated the effect of diets containing 0, 2.5, 5, 7.5 and 10% <em>Arthrospir platensis </em>on the hematological and immune parametersof Koi carp (<em>Cyprinus carpio</em>). A completely randomized experimental design was developed with five treatments and three replicates for 8 weeks. At the end of experiment, the hematological and, immune system parameters were measured and analyzed. The results revealed that supplementation of <em>Arthrospira platensis</em> in diets results in higher percentage of Hb, Hct, RBC and WBC. In addition, a significant increasein the levels of IgM, lysozyme and C4 complement levels were found in fish fed with 10% <em>Arthrospira platensis </em>(p<0.05). These results revealed that, inclusion of 10% <em>A. platensis</em> in diets did appear to have a significant positive effect on stimulation of immune system of Koi fish.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Fatty acid profile in male and female sex gray mullet (Liza aurata) in the southern shores of the Caspian SeaFatty acid profile in male and female sex gray mullet (Liza aurata) in the southern shores of the Caspian Sea333911352010.22092/isfj.2017.113520FAM NorouziM Bagheri TavaniJournal Article20171105The present study aimed to assess fatty acid profile in filet of male and female gray mullet from the southern region of the Caspian Sea. A total of 100 fish (55 male and 45 female) were caught from 10 stations in three coastal provinces of the Caspian Sea. Carcasses were analyzed according to the standard AOAC methods, and fatty acids were measured using gas chromatography. Mean protein, fat, moisture, and ash in golden gray mullet were 22.18±1.98, 3.08±1.21, 78.02±2, 1.40±0.43 respectively. A total of 28 fatty acids were identified, including 10 saturated, 8 monounsaturated, and 10 polyunsaturated fatty acids. Total amount of SFA fatty acids were 27.63, 24.95, 36.02 grams per 100 grams fat, respectively in male fish, and 30.39, 30.26, 29.42 grams per 100 grams fat respectively, in female fish, with a significant difference between them. The most prevalent SFA fatty acids included palmitic, oleic, and docosahexaenoic in fish muscles. Omega 3, omega 6, and omega 9 fatty acids in golden gray mullet comprised 29.61, 3.31, 11.17 percentage of total fatty acids respectively, and were significantly higher in the male species. ω-3/ω-6 and PUFA/SFA ratios were 9.22 and 1.2%, respectively. The sum total of two essential fatty acids in gray mullet (DHA and EPA) was 21.64%, which is important to promotion of health and prevention and recovery from illness in humans.The present study aimed to assess fatty acid profile in filet of male and female gray mullet from the southern region of the Caspian Sea. A total of 100 fish (55 male and 45 female) were caught from 10 stations in three coastal provinces of the Caspian Sea. Carcasses were analyzed according to the standard AOAC methods, and fatty acids were measured using gas chromatography. Mean protein, fat, moisture, and ash in golden gray mullet were 22.18±1.98, 3.08±1.21, 78.02±2, 1.40±0.43 respectively. A total of 28 fatty acids were identified, including 10 saturated, 8 monounsaturated, and 10 polyunsaturated fatty acids. Total amount of SFA fatty acids were 27.63, 24.95, 36.02 grams per 100 grams fat, respectively in male fish, and 30.39, 30.26, 29.42 grams per 100 grams fat respectively, in female fish, with a significant difference between them. The most prevalent SFA fatty acids included palmitic, oleic, and docosahexaenoic in fish muscles. Omega 3, omega 6, and omega 9 fatty acids in golden gray mullet comprised 29.61, 3.31, 11.17 percentage of total fatty acids respectively, and were significantly higher in the male species. ω-3/ω-6 and PUFA/SFA ratios were 9.22 and 1.2%, respectively. The sum total of two essential fatty acids in gray mullet (DHA and EPA) was 21.64%, which is important to promotion of health and prevention and recovery from illness in humans.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Assessment of antibacterial activity of Origanum vulgare extract and nanoform Origanum vulgare extract in comparison with florfenicol and nanoform florfenicol against Streptococcus iniaeAssessment of antibacterial activity of Origanum vulgare extract and nanoform Origanum vulgare extract in comparison with florfenicol and nanoform florfenicol against Streptococcus iniae415011352110.22092/isfj.2017.113521FAMasoud HaghighiS.M.E FakharzadehSaltanat Najar LashgariB PourmolaeiJournal Article20171105The aim of this study was to evaluate <em>in vitro</em> the antibacterial effects of hydro-alcoholic extract of aerial parts of <em>Origanum vulgare</em> and nanoform <em>Origanum vulgare</em> extract on <em>Streptococcus iniae</em>, the causative agent of Streptococcosis in fish, in comparison with the effects of florfenicol and nonoform florfenicol on this bacteria. The results showed that the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of <em>Origanum vulgare</em> extract was 1000 µg/ml whereas, the MIC and MBC of nanoform <em>Origanum vulgare</em> extract were 250 and 500 µg/ml, respectively. These results indicated that the inhibitory and bactericidal effects of <em>Origanum vulgare</em> extract was less than the nanoform <em>Origanum vulgare</em> extract. The MIC and MBC of florfenicol was 2.5 µg/ml while, the MIC and MBC of nanoform florfenicol were 0.625 and 1.25 µg/ml respectively. The results showed that the zones of inhibition for <em>Origanum vulgare </em>extract, nanoform <em>Origanum vulgare</em> extract, florfenicol and nanoform florfenicol were 21.70±0.6, 26.06±0.7,31.63±0.4 and 32.80±1.1 mm, respectively. These results indicated that <em>Origanum vulgare</em> extract had antibacterial effects which were less than the effects of nanoform <em>Origanum vulgare</em> extract, florfenicol and nanoform florfenicol on <em>Streptococcus iniae</em>.In this study, the nanoform florfenicol was the most effective substance for controlling <em>Streptococcus iniae</em> infection.The aim of this study was to evaluate <em>in vitro</em> the antibacterial effects of hydro-alcoholic extract of aerial parts of <em>Origanum vulgare</em> and nanoform <em>Origanum vulgare</em> extract on <em>Streptococcus iniae</em>, the causative agent of Streptococcosis in fish, in comparison with the effects of florfenicol and nonoform florfenicol on this bacteria. The results showed that the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of <em>Origanum vulgare</em> extract was 1000 µg/ml whereas, the MIC and MBC of nanoform <em>Origanum vulgare</em> extract were 250 and 500 µg/ml, respectively. These results indicated that the inhibitory and bactericidal effects of <em>Origanum vulgare</em> extract was less than the nanoform <em>Origanum vulgare</em> extract. The MIC and MBC of florfenicol was 2.5 µg/ml while, the MIC and MBC of nanoform florfenicol were 0.625 and 1.25 µg/ml respectively. The results showed that the zones of inhibition for <em>Origanum vulgare </em>extract, nanoform <em>Origanum vulgare</em> extract, florfenicol and nanoform florfenicol were 21.70±0.6, 26.06±0.7,31.63±0.4 and 32.80±1.1 mm, respectively. These results indicated that <em>Origanum vulgare</em> extract had antibacterial effects which were less than the effects of nanoform <em>Origanum vulgare</em> extract, florfenicol and nanoform florfenicol on <em>Streptococcus iniae</em>.In this study, the nanoform florfenicol was the most effective substance for controlling <em>Streptococcus iniae</em> infection.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Physiochemical and textural properties of Common carp fermented sausages inoculated with mixed starter cultures of Pediococcus pentosaceus and Lactobacillus plantarumPhysiochemical and textural properties of Common carp fermented sausages inoculated with mixed starter cultures of Pediococcus pentosaceus and Lactobacillus plantarum516311352210.22092/isfj.2017.113522FAA JafarpourF YazdanprastR SafariJournal Article20171105In this study, the effects of inoculation of two lactic acid bacteria including <em>Pediococcus pentosaceu </em>and<em> Lactobacillus plantarum</em> were evaluated on the fermented fish sausages produced from Common carp, as a model, at incubation temperature of 35˚C. Common carp fermented sausages were prepared from minced fish, salt (3%), glucose (3%) and the combination of two starter cultures of bacteria (6 log CFU/g) and fermentation occurred during the 48 h incubation. Soluble peptides, pH, phsycrophilic and lactic acid bacterial counts, proximate composition (protein, lipid, moisture and ash), texture profile analysis and color of fermented sausages were measured and compared to the non-inoculated sausages (the control group) at 0, 24 and 48 h after incubation. According to the results, lactic acid bacteria showed rapid growth after 48 h fermentation which led to the significant decrease in pH from initial values of around 6.9 to 4.4 (P<0.05) and the significant decrease in growth and proliferation of phsycrophilic <em>Pseudomonas</em> (P<0.05). The amounts of soluble peptides significantly increased in sausages that were inoculated with the mixed culture as compared to the control group (P<0.05). In terms of texture, the hardness and chewiness of inoculated sausages were higher than that of the control group (P<0.05). In terms of color parameters, the lightness (<em>L</em>*) and redness (<em>a</em>*) of both types of sausages were significantly increased after incubation, whereas the yellowness (<em>b</em>*) was significantly decreased (P<0.05). It can be concluded that fermented sausages inoculated with mixed starter cultures had more favorable physiochemical characteristics and textural properties as compared to the control group. <br /> In this study, the effects of inoculation of two lactic acid bacteria including <em>Pediococcus pentosaceu </em>and<em> Lactobacillus plantarum</em> were evaluated on the fermented fish sausages produced from Common carp, as a model, at incubation temperature of 35˚C. Common carp fermented sausages were prepared from minced fish, salt (3%), glucose (3%) and the combination of two starter cultures of bacteria (6 log CFU/g) and fermentation occurred during the 48 h incubation. Soluble peptides, pH, phsycrophilic and lactic acid bacterial counts, proximate composition (protein, lipid, moisture and ash), texture profile analysis and color of fermented sausages were measured and compared to the non-inoculated sausages (the control group) at 0, 24 and 48 h after incubation. According to the results, lactic acid bacteria showed rapid growth after 48 h fermentation which led to the significant decrease in pH from initial values of around 6.9 to 4.4 (P<0.05) and the significant decrease in growth and proliferation of phsycrophilic <em>Pseudomonas</em> (P<0.05). The amounts of soluble peptides significantly increased in sausages that were inoculated with the mixed culture as compared to the control group (P<0.05). In terms of texture, the hardness and chewiness of inoculated sausages were higher than that of the control group (P<0.05). In terms of color parameters, the lightness (<em>L</em>*) and redness (<em>a</em>*) of both types of sausages were significantly increased after incubation, whereas the yellowness (<em>b</em>*) was significantly decreased (P<0.05). It can be concluded that fermented sausages inoculated with mixed starter cultures had more favorable physiochemical characteristics and textural properties as compared to the control group. <br /> Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Impact of stocking density on whiteleg shrimp (Litopenaeus vannamei, Boone, 1931) culture in brackish water of the Caspian SeaImpact of stocking density on whiteleg shrimp (Litopenaeus vannamei, Boone, 1931) culture in brackish water of the Caspian Sea657511352310.22092/isfj.2017.113523FAS.M.Vahid FarabiIFRO, CSERCA MatinfarIFROA.A SalehiIFRO, CSERCM SharifianifroJournal Article20171105Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Effect of partial substitution of fishmeal by red algae Gracilaria pygmaea on the growth performance, hematology and serum biochemistry parameters of Asian Seabass Lates calcarifer (Bloch, 1790)Effect of partial substitution of fishmeal by red algae Gracilaria pygmaea on the growth performance, hematology and serum biochemistry parameters of Asian Seabass Lates calcarifer (Bloch, 1790)778911352410.22092/isfj.2017.113524FAA FarhoudiI SourinejadM Nafisi BahabadiM.M SajjadiJournal Article20171105Effects of dietary supplementation of red macroalgae <em>Gracilaria pygmea</em> on the growth performance, hematology and serum biochemistry parameters of Asian Seabass <em>Lates calcarifer</em> with initial fish weight of 28±5.9 g were investigated in a 6-week experiment. The experimental diets were composed of the positive control diet (containing fishmeal), the negative control diet (containing fishmeal-soy protein) and three diets which supplemented with varying leves of red algae (3, 6 and 9%). Results indicated that daily growth rate, feed conversion ratio and final weight of fish not only were not negatively affected by different substitution levels of red algae, but also the final weight of fish fed the diet containing 6% red algae was higher than the other experimental diets. Condition factor of the negative control group and the specific growth rate of the treatment containing 6% red algae were significantly higher than those of the other experimental diets (P<0.05). Results of hemoglobin, hematocrit, White Blood Cell (WBC) count and WBC differential count were not significantly different among various treatments (P>0.05). Among the examined serum biochemistry parameters of Asian Seabass, only glucose was not significantly affected by various dietary treatments (P>0.05). The cholesterol levels of the negative control group and fish fed the diet containing 9% red algae were similar with each other and were significantly higher than the other treatments (P<0.05). The lowest level of albumin was observed in the treatment containing 6% red algae, whearese the lowest amounts of total protein, triglyceride and cholesterol were observed in the treatment containing 3% red algae. Overall, it seems that partial substitution of expensive fishmeal by red algae <em>Gracilaria pygmaea</em> in the diet of carnivorous Asian Seabass exerted no negative effects on the growth and immunity parameters. Moreover, some growth parameters, hematological and serum biochemical parameters of blood were improved by the diet containing 6% red algae.Effects of dietary supplementation of red macroalgae <em>Gracilaria pygmea</em> on the growth performance, hematology and serum biochemistry parameters of Asian Seabass <em>Lates calcarifer</em> with initial fish weight of 28±5.9 g were investigated in a 6-week experiment. The experimental diets were composed of the positive control diet (containing fishmeal), the negative control diet (containing fishmeal-soy protein) and three diets which supplemented with varying leves of red algae (3, 6 and 9%). Results indicated that daily growth rate, feed conversion ratio and final weight of fish not only were not negatively affected by different substitution levels of red algae, but also the final weight of fish fed the diet containing 6% red algae was higher than the other experimental diets. Condition factor of the negative control group and the specific growth rate of the treatment containing 6% red algae were significantly higher than those of the other experimental diets (P<0.05). Results of hemoglobin, hematocrit, White Blood Cell (WBC) count and WBC differential count were not significantly different among various treatments (P>0.05). Among the examined serum biochemistry parameters of Asian Seabass, only glucose was not significantly affected by various dietary treatments (P>0.05). The cholesterol levels of the negative control group and fish fed the diet containing 9% red algae were similar with each other and were significantly higher than the other treatments (P<0.05). The lowest level of albumin was observed in the treatment containing 6% red algae, whearese the lowest amounts of total protein, triglyceride and cholesterol were observed in the treatment containing 3% red algae. Overall, it seems that partial substitution of expensive fishmeal by red algae <em>Gracilaria pygmaea</em> in the diet of carnivorous Asian Seabass exerted no negative effects on the growth and immunity parameters. Moreover, some growth parameters, hematological and serum biochemical parameters of blood were improved by the diet containing 6% red algae.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Reproductive Biology of Kingsoldier Bream (Argyrops spinifer) in the Persian GulfReproductive Biology of Kingsoldier Bream (Argyrops spinifer) in the Persian Gulf9110411352510.22092/isfj.2017.113525FAS HamzehY KeivanyN Mahboobi SoofianiKh Aein JamshidJournal Article20171105Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823The interaction effect between methionine and L-carnitine on growth parameters, carcass composition and blood indices in rainbow trout Oncorhynchus mykissThe interaction effect between methionine and L-carnitine on growth parameters, carcass composition and blood indices in rainbow trout Oncorhynchus mykiss10511811352610.22092/isfj.2017.113526FAA KeramatA AbolfazliJournal Article20171105The main goal of the current study is to investigate the effects of L-carnitine supplement mixed with methionine on fat metabolism efficacy and consequently growth performance improvement in rainbow trout. In order to study the interaction effects between methionine and L-carnitine, three levels of methionine (0.5, 1 and 1.5%) and three levels of L-carnitine (0.5, 1 and 1.5 g/kg) were added to a an experimental diet. This led to nine experimental diets. The results showed that growth parameters such as final weight, feed conversion rate and specific growth rate were influenced by addition methionine (p<0.05). However, L-carnitine supplementation did not change growth parameters in rainbow trout, except final weight (p>0.05). The result also revealed that in general fish fed diets containing methionine and L-carnitine showed a better growth performance in comparison to control diet (without methionine and L-carnitine). Inclusion of both methionine and L-carnitine reduced blood glucose concentration compared with control diet (P< 0.05). Carcass protein level increase by addition of both methionine and L-carnitine compared to control diet (p< 0.05). Dislike to protein, administration of both methionine and L-carnitine reduced fat content of carcass compared to control diet (p< 0.05). In conclusion, administration of L-carnitine along with methionine at low level had a larger positive impact on growth and musscle composition in rainbow trout.
<strong> </strong>The main goal of the current study is to investigate the effects of L-carnitine supplement mixed with methionine on fat metabolism efficacy and consequently growth performance improvement in rainbow trout. In order to study the interaction effects between methionine and L-carnitine, three levels of methionine (0.5, 1 and 1.5%) and three levels of L-carnitine (0.5, 1 and 1.5 g/kg) were added to a an experimental diet. This led to nine experimental diets. The results showed that growth parameters such as final weight, feed conversion rate and specific growth rate were influenced by addition methionine (p<0.05). However, L-carnitine supplementation did not change growth parameters in rainbow trout, except final weight (p>0.05). The result also revealed that in general fish fed diets containing methionine and L-carnitine showed a better growth performance in comparison to control diet (without methionine and L-carnitine). Inclusion of both methionine and L-carnitine reduced blood glucose concentration compared with control diet (P< 0.05). Carcass protein level increase by addition of both methionine and L-carnitine compared to control diet (p< 0.05). Dislike to protein, administration of both methionine and L-carnitine reduced fat content of carcass compared to control diet (p< 0.05). In conclusion, administration of L-carnitine along with methionine at low level had a larger positive impact on growth and musscle composition in rainbow trout.
<strong> </strong>Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Investigation of factors affecting fresh fish consumption in IranInvestigation of factors affecting fresh fish consumption in Iran11912711352710.22092/isfj.2017.113527FAS ZiaeeKh Samare HashemiS.A. Samare HashemiJournal Article20171105The present study has studied factors influencing the consumption of fresh fish in Iran. Information required for the study, included cost-income data of 38252 urban and rural households that has been collected in 2015 by the Statistical Center of Iran. Due to the nature of the data, first from the Tobit, Hackman and double Hurdle models based on likelihood ratio and Wong tests along with sign coefficients and their significance, double Hurdle model was selected as an appropriate model. Results showed that age, education and gender of household head, income status, living area and distance to the sea have a significant positive impact on participation (decision to consume fresh fish) and head`s age, head`s education, wife`s education, family size and monthly income of the household and the price index for meat and poultry have positive effect and the price of fish has a significant negative impact on the consumption of fish. So more awareness and expand access centers can be effective in increasing consumption. <br /> The present study has studied factors influencing the consumption of fresh fish in Iran. Information required for the study, included cost-income data of 38252 urban and rural households that has been collected in 2015 by the Statistical Center of Iran. Due to the nature of the data, first from the Tobit, Hackman and double Hurdle models based on likelihood ratio and Wong tests along with sign coefficients and their significance, double Hurdle model was selected as an appropriate model. Results showed that age, education and gender of household head, income status, living area and distance to the sea have a significant positive impact on participation (decision to consume fresh fish) and head`s age, head`s education, wife`s education, family size and monthly income of the household and the price index for meat and poultry have positive effect and the price of fish has a significant negative impact on the consumption of fish. So more awareness and expand access centers can be effective in increasing consumption. <br /> Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Effects of organic and mineral selenium supplementation on growth performance, heamatological and immunological properties of rainbow trout (Oncorhynchus mykiss)Effects of organic and mineral selenium supplementation on growth performance, heamatological and immunological properties of rainbow trout (Oncorhynchus mykiss)12913811352810.22092/isfj.2017.113528FAK NazariM Shamsaie MehrjanN EilaI SharifporA KamaliJournal Article20171105The present study was carried out to evaluate the effects of various amounts of organic and mineral selenium supplementation on growth performance (including condition factor (CF), specific growth rate (SGR), and weight gain (WG)), heamatological indices and liver histopathology. Nine dietary treatments were prepared and supplemented with selenium at 0 (control group (T0)), 0.15 (T1), 0.3 (T2), 0.45 (T3) and 0.6 g/kg of fish diet (T4). Fish were fed with formulated diet during 60 days of experiment. The average weight gain of the T3 group that was fed with organic selenium (214±18.6 g) was significantly higher (P<0.05) than the control group (154/6±25 g). The average SGR values in T2, T3 and T4 that were fed with organic selenium and the group T2 that was fed with mineral selenium were significantly higher than the control group (P<0.05). The concentration of IgM was significantly higher (P<0.05) in T1 (55.05±11.6 mg/dL) that was fed with organic selenium as compared to control group (46.9±6.6 mg/dL). Lysozyme activity in T3 (1066.7±24.5 unit/g) that was fed with organic selenium was significantly higher (P<0.05) than the control group (838.5±11 unit/g). The highest amounts of C3 (34.8±28.4 mg/dL) and C4 (31.1±7.3 mg/dL) were observed in T4 group that was fed with organic selenium. These results indicated that dietary supplementation of organic selenium up to 0.45 mg/kg of fish diet represented an appropriate ratio of growth factors, immune responses and liver pathological properties in rainbowThe present study was carried out to evaluate the effects of various amounts of organic and mineral selenium supplementation on growth performance (including condition factor (CF), specific growth rate (SGR), and weight gain (WG)), heamatological indices and liver histopathology. Nine dietary treatments were prepared and supplemented with selenium at 0 (control group (T0)), 0.15 (T1), 0.3 (T2), 0.45 (T3) and 0.6 g/kg of fish diet (T4). Fish were fed with formulated diet during 60 days of experiment. The average weight gain of the T3 group that was fed with organic selenium (214±18.6 g) was significantly higher (P<0.05) than the control group (154/6±25 g). The average SGR values in T2, T3 and T4 that were fed with organic selenium and the group T2 that was fed with mineral selenium were significantly higher than the control group (P<0.05). The concentration of IgM was significantly higher (P<0.05) in T1 (55.05±11.6 mg/dL) that was fed with organic selenium as compared to control group (46.9±6.6 mg/dL). Lysozyme activity in T3 (1066.7±24.5 unit/g) that was fed with organic selenium was significantly higher (P<0.05) than the control group (838.5±11 unit/g). The highest amounts of C3 (34.8±28.4 mg/dL) and C4 (31.1±7.3 mg/dL) were observed in T4 group that was fed with organic selenium. These results indicated that dietary supplementation of organic selenium up to 0.45 mg/kg of fish diet represented an appropriate ratio of growth factors, immune responses and liver pathological properties in rainbowAgricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Identifying color morphotypes of the species Leptodius exaratus (Brachyuran: Xanthidae) based on the molecular and electron microscopy studiesIdentifying color morphotypes of the species Leptodius exaratus (Brachyuran: Xanthidae) based on the molecular and electron microscopy studies13914811352910.22092/isfj.2017.113529FAF ChenariS.M.B NabaviM.A. SalariA SavariH ZolgharneinJournal Article20171105According to morphological studies, <em>Leptodius exaratus</em> is one of the most common brachyuran species and the only known species of this genus which have been found in intertidal rocky shores along the Persian Gulf coast. Since the existence of hidden species among the crustacean species are very common, the aim of this study is to investigate the probability of hidden species identification among eight color morphotypes that were identified as <em>Leptodius exaratus</em> species in morphological studies. For this purpose, eight color morphotypes of <em>Leptodius exaratus</em> species were collected from rocky shores of Bushehr province. Then, the first male gonopods were separated and photographed by Scanning Electron Microscopy (SEM). For conducting molecular studies, DNA was extracted with a phenol-chloroform mixture and mitochondrial Cytochrome Oxidase subunit I (COI) gene was amplified and sequenced. The results showed that mitochondrial COI nucleotide sequences were not identical in all color morphotypes. These results were reflected in topology of evolutionary trees for both analyses (maximum likelihood, bayesian). Studying the ultrastructure of the apical segments of the first male gonopods showed fundamental differences in the apical organs of some color morphotypes. These results indicated that all samples did not belong to a single species and there were hidden species among them. Molecular evidence also showed that mitochondrial COI nucleotide sequences of eight color morphotypes of <em>Leptodius</em> <em>exaratus</em> from the Persian Gulf and the registered sequence of such species from Indonesia were in separate clusters. Identification based on the morphology of the first male gonopods using scanning electron microscopy (SEM) and the COI gene were useful research methods in the present study.According to morphological studies, <em>Leptodius exaratus</em> is one of the most common brachyuran species and the only known species of this genus which have been found in intertidal rocky shores along the Persian Gulf coast. Since the existence of hidden species among the crustacean species are very common, the aim of this study is to investigate the probability of hidden species identification among eight color morphotypes that were identified as <em>Leptodius exaratus</em> species in morphological studies. For this purpose, eight color morphotypes of <em>Leptodius exaratus</em> species were collected from rocky shores of Bushehr province. Then, the first male gonopods were separated and photographed by Scanning Electron Microscopy (SEM). For conducting molecular studies, DNA was extracted with a phenol-chloroform mixture and mitochondrial Cytochrome Oxidase subunit I (COI) gene was amplified and sequenced. The results showed that mitochondrial COI nucleotide sequences were not identical in all color morphotypes. These results were reflected in topology of evolutionary trees for both analyses (maximum likelihood, bayesian). Studying the ultrastructure of the apical segments of the first male gonopods showed fundamental differences in the apical organs of some color morphotypes. These results indicated that all samples did not belong to a single species and there were hidden species among them. Molecular evidence also showed that mitochondrial COI nucleotide sequences of eight color morphotypes of <em>Leptodius</em> <em>exaratus</em> from the Persian Gulf and the registered sequence of such species from Indonesia were in separate clusters. Identification based on the morphology of the first male gonopods using scanning electron microscopy (SEM) and the COI gene were useful research methods in the present study.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Faunistic study of Chironmidae in the Hablehrood RiverFaunistic study of Chironmidae in the Hablehrood River14915811355610.22092/isfj.2017.113556FAS ArkiaS Yousefi SiahkalroodiN KheradpirA. KaramiJournal Article20171108Chironmidae is one of the zoo-ecological unique group in the aquatic ecosystem. These insects are among the frequent larvae in shallow areas of the lakes, pools and road which has a dense cover of aquatic plants. This study was conducted to identify the Chironomidae living in Hablehrood stream. Sampling programmed seasonally for four selected pilots (Zarrindasht, Simindasht, KHomadeh and Enzaha) by dredge in three replications for each pilot in 2015. Samples were transferred in ethanol 70% to the laboratory. In the laboratory, larvae were separated from other substrate elements, after calcifying the samples, they were permanently fixed. Identification was done by use of available keys to genus level. The result showed that three genus hironomus, Polypedilum and Stritochironomus belonged to sub-family Chironominae were collected from Hablehrood. <br /> Chironmidae is one of the zoo-ecological unique group in the aquatic ecosystem. These insects are among the frequent larvae in shallow areas of the lakes, pools and road which has a dense cover of aquatic plants. This study was conducted to identify the Chironomidae living in Hablehrood stream. Sampling programmed seasonally for four selected pilots (Zarrindasht, Simindasht, KHomadeh and Enzaha) by dredge in three replications for each pilot in 2015. Samples were transferred in ethanol 70% to the laboratory. In the laboratory, larvae were separated from other substrate elements, after calcifying the samples, they were permanently fixed. Identification was done by use of available keys to genus level. The result showed that three genus hironomus, Polypedilum and Stritochironomus belonged to sub-family Chironominae were collected from Hablehrood. <br /> Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Site selection for suitable fish cage culture in the Iranian waters of the Caspian SeaSite selection for suitable fish cage culture in the Iranian waters of the Caspian Sea15916911355710.22092/isfj.2017.113557FAGh DrayanabardS.M.V FarabiH FazliJournal Article20171108Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Effects of dietary L-carnitine on growth performance, body composition and antioxidant status of beluga (Huso huso) and comparison with Biomar dietEffects of dietary L-carnitine on growth performance, body composition and antioxidant status of beluga (Huso huso) and comparison with Biomar diet17118311355810.22092/isfj.2017.113558FAM MohseniM PourkazemiR KazemiR TaatiJournal Article20171108A 17-week feeding trial was carried out to evaluate the effects of dietary L-carnitine level in beluga, <em>Huso huso</em>. A total of fish averaging 1247 ± 15.6 g (mean ± SD) were randomly distributed into 21 fibreglass tanks, and each tank holding 10 fish was then randomly assigned to one of three replicates of seven diets with 50, 150, 350, 650, 950 and 1250 mg L-carnitine kg<sup>-1</sup> diet or control diet (Biomar). At the end of 17 weeks of feeding trial, average weight gain (WG), feed efficiency (FE), protein efficiency ratio (PER) and condition factor (CF) of fish fed 350 mg kg<sup>-1</sup> diet were significantly (P < 0.05) higher than those of fish fed 50, 150, 950 and 1250 mg kg<sup>-1</sup> diets. WG, FE, PER and CF of beluga fed 650 mg kg<sup>-1</sup> diet or control diet (Biomar) were also significantly higher than those of fish fed 50, 950 and 1250 mg kg<sup>-1 </sup>diets. Whole body and muscle protein were significantly improved by the elevation of dietary L-carnitine level up to 350 mg kg<sup>-1</sup>. Liver superoxide dismutase and glutathione peroxidase activities of fish fed 350 and 650 mg kg<sup>-1 </sup>diets were significantly higher than those of fish fed other diets. The dietary L-carnitine level of 350mg kg<sup>-1</sup> diet could improve growth performance, feed utilization, protein-sparing effects of lipid, immune response, antioxidant defence system and reproductive success.A 17-week feeding trial was carried out to evaluate the effects of dietary L-carnitine level in beluga, <em>Huso huso</em>. A total of fish averaging 1247 ± 15.6 g (mean ± SD) were randomly distributed into 21 fibreglass tanks, and each tank holding 10 fish was then randomly assigned to one of three replicates of seven diets with 50, 150, 350, 650, 950 and 1250 mg L-carnitine kg<sup>-1</sup> diet or control diet (Biomar). At the end of 17 weeks of feeding trial, average weight gain (WG), feed efficiency (FE), protein efficiency ratio (PER) and condition factor (CF) of fish fed 350 mg kg<sup>-1</sup> diet were significantly (P < 0.05) higher than those of fish fed 50, 150, 950 and 1250 mg kg<sup>-1</sup> diets. WG, FE, PER and CF of beluga fed 650 mg kg<sup>-1</sup> diet or control diet (Biomar) were also significantly higher than those of fish fed 50, 950 and 1250 mg kg<sup>-1 </sup>diets. Whole body and muscle protein were significantly improved by the elevation of dietary L-carnitine level up to 350 mg kg<sup>-1</sup>. Liver superoxide dismutase and glutathione peroxidase activities of fish fed 350 and 650 mg kg<sup>-1 </sup>diets were significantly higher than those of fish fed other diets. The dietary L-carnitine level of 350mg kg<sup>-1</sup> diet could improve growth performance, feed utilization, protein-sparing effects of lipid, immune response, antioxidant defence system and reproductive success.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Bioaccumulation of heavy metals, Ni, Cd and Pb in muscle, liver and caviar of farmed Acipenser baerii from Khuozestan ProvinceBioaccumulation of heavy metals, Ni, Cd and Pb in muscle, liver and caviar of farmed Acipenser baerii from Khuozestan Province18519211355910.22092/isfj.2017.113559FAA Mohammad SalehiVelayatzadeh VelayatzadehN Karami RadJournal Article20171108The presence of heavy metals in our environment has been of great concern because of their toxicity when their concentration is more than the permissible level. These metals enter in the environment by different ways e.g. industrial activities. This study was carried out to determine and comparison of heavy metals Cd, Pb and Ni in muscle, liver and caviar of farmed <em>Acipenser baerii</em> from Khuozestan Province, 2014. 30 samples of <em>Acipenser baerii</em> were prepared randomly from five ponds of Hantoshzadeh breeding. Heavy metal levels in fish samples were analyzed by Perkin Elmer 4100 zl atomic absorption. Data analysis was performed with the software SPSS17 and comparison of the mean to T-test. The highest concentration of Ni, Cd and Pb were 1.10±0.23, 0.041±0.002 and 0.35±0.02 µ/Kg in liver of <em>Acipenser baerii</em>. The lowest concentration of this metals were obtained 0.08±0.003, 0.017±0.002 and 0.11±0.02 µ/Kg in caviar this species. Concentration of heavy metals in the liver of <em>Acipenser baerii</em> was higher than muscle and caviar. In this study concentration of Ni, Cd and Pb in muscle and caviar of <em>Acipenser baerii</em> were lower than comparison of WHO, FDA, MAFF and NHMRC standards.The presence of heavy metals in our environment has been of great concern because of their toxicity when their concentration is more than the permissible level. These metals enter in the environment by different ways e.g. industrial activities. This study was carried out to determine and comparison of heavy metals Cd, Pb and Ni in muscle, liver and caviar of farmed <em>Acipenser baerii</em> from Khuozestan Province, 2014. 30 samples of <em>Acipenser baerii</em> were prepared randomly from five ponds of Hantoshzadeh breeding. Heavy metal levels in fish samples were analyzed by Perkin Elmer 4100 zl atomic absorption. Data analysis was performed with the software SPSS17 and comparison of the mean to T-test. The highest concentration of Ni, Cd and Pb were 1.10±0.23, 0.041±0.002 and 0.35±0.02 µ/Kg in liver of <em>Acipenser baerii</em>. The lowest concentration of this metals were obtained 0.08±0.003, 0.017±0.002 and 0.11±0.02 µ/Kg in caviar this species. Concentration of heavy metals in the liver of <em>Acipenser baerii</em> was higher than muscle and caviar. In this study concentration of Ni, Cd and Pb in muscle and caviar of <em>Acipenser baerii</em> were lower than comparison of WHO, FDA, MAFF and NHMRC standards.Agricultural Research,Education and Extension OrganizationIranian Scientific Fisheries Journal1026-135426320170823Comparison of three different traps for catching oriental river prawn (Macrobrachium nipponense)Comparison of three different traps for catching oriental river prawn (Macrobrachium nipponense)19319611356010.22092/isfj.2017.113560FAA.A. KhanipourM AminiA. NooriE KamraniJournal Article20171108One of the comparison methods for evaluating fishing gears efficiencies is based on Catch per Unit Effort (CPUE). In this study, we used three different types of traps for catching oriental river prawn (<em>Macrobrachium nipponense</em>). Capture efficiencies and catch rates of cylindrical pot, opera house trap, and Hokkaido pot were compared with each other during the investigation in Anzali Lagoon. The results indicated that there is a significant difference (P˂0/05) among the amounts of CPUE of cylindrical pot (0.24±0.12 gr), opera house trap (4.17±0.84 gr) and Hokkaido pot (1.58±0.41 gr). There was a significant difference between the results of length frequencies of captured prawns that was trapped by cylindrical pot and opera house trap (P<0.05). Hokkaido, cylindrical and opera house traps were not significantly different from each other for length frequencies. The results indicated that opera house trap was an appropriate fishing gear for catching oriental river prawn because it had higher catch rates and caught prawns with higher length frequencies.One of the comparison methods for evaluating fishing gears efficiencies is based on Catch per Unit Effort (CPUE). In this study, we used three different types of traps for catching oriental river prawn (<em>Macrobrachium nipponense</em>). Capture efficiencies and catch rates of cylindrical pot, opera house trap, and Hokkaido pot were compared with each other during the investigation in Anzali Lagoon. The results indicated that there is a significant difference (P˂0/05) among the amounts of CPUE of cylindrical pot (0.24±0.12 gr), opera house trap (4.17±0.84 gr) and Hokkaido pot (1.58±0.41 gr). There was a significant difference between the results of length frequencies of captured prawns that was trapped by cylindrical pot and opera house trap (P<0.05). Hokkaido, cylindrical and opera house traps were not significantly different from each other for length frequencies. The results indicated that opera house trap was an appropriate fishing gear for catching oriental river prawn because it had higher catch rates and caught prawns with higher length frequencies.