Production of single cell protein from stickwater of kilka fish meal factory using Lactobacillus acidophilus and Aspergillus niger

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Abstract

We investigated production of single cell protein (SCP) from stickwater of kilka fish meal
factory as medium using Lactobacillus acidophilus and Aspergillus niger. Stickwater was
used instead of the standard media of bacterium and fungus in a batch culture method.
Amount of biomass, COD, RNA and protein in the bacterium and fungus in control and
stickwater treatments were investigated. In maximum growth time, amino acids profile of the
bacterium and fungus were measured and compared between treatments. Bacterial biomass
production in the control and stickwater treatments were 3.16 and 5.12g/l, COD reduction was
33270 and 53330mg/l, the measured RNA were 15.27% and 15.04%, the amount of protein
were 71.13% and 68.37%, respectively. The difference between bacterium and fungus
biomass production was slight. We found that the amount of the fungus biomass in control
and stickwater were 6.31 and 7.28g/l, COD reduction were 47800 and 55200mg/l, RNA was
9.36% and 9.09%, the amount of protein were 51.36% and 48.66%, respectively. In both
bacterium and fungus, the maximum and minimum amount of amino acid of the control and
stickwater was glutamic acid and methionin. The amount of methionin in bacterium was not
different with fish meal and FAO reference and in fungus was a little lower than FAO
reference. According to the results, application of pure stickwater was suitable for production
of Lactobacillus acidophilus and Aspergillus niger.

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Iranian Scientific Fisheries Journal
Volume 19, Issue 4
May and June 2011
Pages 21-32

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