Daphnia pulex through physical and chemical factors

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Abstract

Regarding the key role and importance of Daphnia in aquaculture, particularly for survival
and adaptation of fishes in larval stages, a comprehensive study on hatching rate of Daphnia
ephippial eggs was implemented to obtain an optimum technique. The effects of abiotic
factors was investigated on the hatching response of Daphnia pulex diapausing eggs collected
from live food ponds in Cultivation and Breeding Centre of Gorgan. After isolation, the eggs
were kept in dry and wet conditions at 4°C for 2 months in darkness. Following the preincubation
period, the eggs in each group were subdivided into 2 parts and treated with 1%
NaOCl and distilled water. The effect of temperature levels (15 C, 20 C, 25 C) and
photoperiod levels (12L: 12D, 24L: 0D) were identified on the hatching of eggs in AdaM
medium during 15 days. Results indicated that the exposure of wet-kept eggs to 12-hour
photoperiod and 25 C without soaking in 1% NaOCl, caused the maximum rate and speed of
hatching.

Keywords

Iranian Scientific Fisheries Journal
Volume 19, Issue 4
May and June 2011
Pages 67-76

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