Tracking of Hepatopancreatic parvo-like virus (HPV) disease in Litopenaeus vannamei of the hatcheries in the Bushehr Province

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Abstract

Presence of hepatopancreatic parvo-like vines (HPV) disease was assessed from June until
October 2009 in Litopenaeus vannamei hatcheries and grow-out farms of the Bushehr
province. Samples were collected from 6 hatcheries and 6 grow-out farms located in coasted
areas. From each hatchery, 100 PL samples with average age PL5-PL8 and 20-30 samples
from each grow-out farm with average age 105 to 120 days were collected. The samples were
divided into three groups one used for gross sign and wet mount with Gimsa, the second
group was preserved in Davidson Fixative and used for histopathology and the third group was
fixed in ethyl alcohol 95% and used for polymerase chain Reaction (PCR). In gross sign 30%-
40% of the shrimp showed different sizes and some were smaller than the others. In the wet
mount group with Gimsa staining of hepatopancrease, the inclusion body with basophilic color
was seen. The histopathology indicated that the hepatopancreatic cell was infected and the
basophilic inclusion body observed in many samples. The PCR examined with IQ 2000 Kit
was negative. The rate of infection (ROI) was 1.1% for hatcheries and 32% for grow-out
farms.

Keywords

Iranian Scientific Fisheries Journal
Volume 19, Issue 4
May and June 2011
Pages 107-118

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