The lethal concentration (LC50) of Zataria multiflora essential oil in fries of rainbow trout (Oncorhynchus mykiss)

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Abstract

This research was carried out to determine LC50 for Zataria multiflora essential oil in
rainbow trout (Oncorhynchus mykiss) fries at 96 hours. In the process, we used the static
O.E.C.D method for 180 rainbow trout fries weighing 5±1 grams in five treatments and one
control with three replicates. During the experiments, the mean (±SD) of water temperature
was kept at 16±1 C, dissolved oxygen (DO) was 7±0.2mg/l and pH was 8±0.4. We used 5-
25ppm of the essential oil and the results were analyzed using probit analysis procedure. The
LC50 96h of Zataria multiflora essential oil was determined as 13.6ppm for rainbow trout
(Oncorhynchus mykiss) fry. Based on the results, the useful maximum concentration of
Zataria multiflora essential oil was less than 10ppm in rainbow trout fry. The results
indicated a narrow therapeutic index for Zataria multiflore essential oil.
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Iranian Scientific Fisheries Journal
Volume 20, Issue 2
March and April 2011
Pages 89-96

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