Genetic variation of Metapenaeus affinis in Persian Gulf coastal waters using microsatellite markers

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Abstract

Genetic diversity of Metapenaeus affinis population from the northern coasts of the Persian
Gulf (Bahrakan, Lifeh-Boosiaf) was studies using microsatellite markers. During September
to October 2007, 60 samples of pleopods tissue of the shrimp were taken and genomic DNA
was extracted by acetate method. PCR was performed on microsatellite primers. To measure
fragment size, samples were run on an 8% polyacrylamid gel. For each microsatellite locus,
using genetic software, Pop Gene and Gene Alex, allele frequency, real and expected
heterozygosity, Fst and Rst and other relevant factors were measured. Of the obtained 5 paired
microsatellite primers, all were polymorphic. The mean observed and effective alleles number
was 7 and 3.67, respectively and also the mean observed and expected heterozygosis was 0.27
and 0.66, respectively. It was also seen that specimens from all regions were not in Hardy-
Weinberg Equibrium in all of the loci. Based on the analysis of molecular variance
(AMOVA) Fst, Rst and Nm were 0.107, 0.372 and 2.092, respectively. The highest genetic
distance was 0.571 and the lowest was 0.561. The present study showed that two different
populations of Metapenaeus affinis are living in the Bahrakan and Lifeh-Boosiaf region
northwest coasts of the Persian Gulf.
*Corresponding author

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Iranian Scientific Fisheries Journal
Volume 20, Issue 3
May and June 2011
Pages 45-54

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