Effect of LHRHA2 and carp pituitary extract hormone on some spermatozoa fertility and quality indices in Barbus sharpeyi

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Abstract

Effects of LHRHA2 hormone in 2.5, 5 and 10μg/kg doses and anti-dopamine
metoclopramide on Barbus sharpeyi spermiation, spermatozoa quality and fertility indices,
chemical (Na+, K+ and Ca+2) and biochemical component (Alkaline phosphates, Glucose and
Triglyceride) of seminal plasma and its osmotic pressure were assessed in comparison to Carp
pituitary extract treatment. Results indicated that the highest incubation survival, hatching
rate, sperm volume, duration of sperm motility as well as the lowest larval deformity were
achieved by injection of LHRHA2 (10μg/kg +2.5mg/kg metoclopramide) and it was
significantly different to others groups. Application of above mentioned treatment had the
highest osmotic pressure with about (Mean ±SD) 324±7.31mOsmol kg-1 among other groups.
Also, the highest level of Na+ was assessed by injection of LHRHA2 (10μg/kg +2.5mg/kg
metoclopramide). Result showed that injection of Carp pituitary extract caused the highest
spermatocrit, K+ value and larval deformity in comparison to other treatments. In conclusion,
optimum sperm quality can be produced by injection of LHRHA2 (10μg/kg + 2.5mg/kg
metoclopramide) in males of B. sharpeyi at 8 hour following hormonal stimulation. Also,
above mentioned treatment caused increasing of incubation survival and decrease of larval
deformity in B. sharpeyi.

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Iranian Scientific Fisheries Journal
Volume 21, Issue 2 - Serial Number 79
March and April 2013
Pages 99-114

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