Biotechnic of brood stocking, artificial propagation and some physiological indices in farmed Acipenser nudiventris

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Abstract

This study was conducted on farmed Acipenser nudiventris at the International Sturgeon
Research Institute from the winter of 2007 till summer 2010. A total of 11 farmed
A. nudiventris specimens (7 male and 4 female) were stocked in groups of 4 fish in eight
fiberglass tanks (4 tons capacity) on the basis of their sex. Males were fed with a diet with no
soybean but containing vitamins. Females were fed with a diet containing soybean and
vitamins with 38-40% protein, 13-15% fat, 19.5-20Mj/kg energy, respectively. GnRH was
used for artificial propagation of Acipenser nudiventris brood stocks twice for females (dose
10μg/kg with 80:20 ration) and during one occasion for males with a dose of 20 μg/kg. The
results of the study indicated that food composition (soybean and vitamins C and E) played a
significant and positive role in the reproduction system for females. No significant effect of
treatment was observed in Testosterone levels between mature (Mean ±SD: 60.6±8.07ng/ml)
and immature (24.42±4.87ng/ml) males, Likewise, no significant differences were found
in17α-Hydroxy Progestrone for female (Mean ±SD: 0.106±0.019ng/ml) and for immature
fishes (0.031±0.006ng/ml) significant differences was detected in the concentration of
Albomine (1.54±0.05mg/dl for females and 1.35±0.07mg/dl for immature females), but there
were no significant differences in other biochemical parameters including Glocose, Cortisol,
Chlosterole and Triglisride in males and females. There were no significant differences in
total weight and length, WBC, RBC, Hb, Hct, MCH, MCHC and MCV parameters in males
and females. This study is a step forward towards the development of artificial breeding and
rearing sturgeon fish in the country.

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