Effect of Dietary Protein and Energy Levels on the Growth and Body Composition of Juvenile Orange-spotted Grouper, Epinephelus coioides

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Abstract

To evaluate the protein and energy requirement of orange-spotted grouper, Epinephelus
coioides fingerlings, nine experimental diets including 3 levels of crude protein (40%, 50%
and 60%) and 3 levels of digestible energy (DE, 14, 16 and 18KJ.g-1), were tested. Triplicate
300 L circular tanks provided with flow-through water system (2L. min-1) and air stone and
each stocked with 20 juvenile of grouper fish (16.79±0.4g) were applied to adopt an
experimental test for 8-weeks rearing period. The stocked fishes were fed experimental diets
to approximate satiation, twice a day. Results showed that the survival rate, hepatosomatic
index (HSI) and condition factor (CF) were independent of the dietary treatments, but final
weight, weight gain, specific growth rate(SGR), feed efficiency (FE), feed conversion ratio
(FCR), protein efficiency ratio (PER) and daily feed intake (DFI) were significantly affected
by protein and digestible energy levels (P0.05) between the treatments. viscerosomatic index(VSI) increased
significantly with the increasing of diet energy level. Body lipid, ash and moisture contents
were affected by dietary protein and energy levels. Body lipid content increased with
increasing dietary energy levels, but carcass ash and moisture decreased. Body protein content
was not affected by dietary energy level, but significantly increased when protein increased
from 40% to 50% at same energy levels (P

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Iranian Scientific Fisheries Journal
Volume 21, Issue 4 - Serial Number 81
May and June 2013
Pages 41-56

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