Short term preservation of spermatozoa of rainbow trout (Oncorhynchus mykiss)

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Abstract

Short term preservation of spermatozoa of salmonid fishes for practical use, increasing of
reproduction potentials and reducing of expenses, has an important role in hatcheries. In this
study spermatozoan motility and fertilization rate of 29 commercially cultured rainbow trout
were examined. Semen was collected by abdominal stripping at the volume of 4.49±1
(mean±SD) ml and sperm concentration was determined by heamocytometer following 1:600
dilution with saline solution (0.9% NaCl). To find the effects of preservation time on
fertilization capacity, samples were diluted with extender (Glucose 54gL-1, Tris 0.5gL-1,
DMSO 9% , Egg yolk 20%) and held at 3 different cool conditions : room temperature
(+15°C), refrigerator (+5°C) and crashed ice (+ 1-2°C) and sperm motility followed up each
30 mins until complete ceasation. Fertilization, hatching and larval stage rate were determined
after 24, 48 and 72 hours. Results showed that the motility of sperm samples held at 15 °C
and 5°C were completely ceased after 2 and 24 hours respectively. However it lasted up to
144 hours at1-2°C.Fertilization, hatching and larval stage rates of sperm samples hold at 1-
2°C were 68.45% , 62.1% and 58.2% (after 24 hours), 53.2% , 43.2% and 40.9% (after 48
hours) and 36.6% , 31.6% and 23.7% after 72 hours, respectively, and all showed a
significant decrease compared with control (90.32%, 83.27% and 81.12% respectively).
Comparing the results showed that holding samples in test tubes at 1-2°C is the best method
for short term storage of rainbow trout spermatozoa.

Keywords

Iranian Scientific Fisheries Journal
Volume 21, Issue 4 - Serial Number 81
May and June 2013
Pages 157-164

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