Evaluation of lysosomal stability and red blood cell membrane fragility in mudskipper (Boleophthalmus dussumieri) as a biomarker of poly aromatic hydrocarbons

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Abstract

This research was carried out to study some physiological responses of mudskipper (i.e.,
Boleophthalmus dussumieri) as a biomarker Poly Aromatic Hydrocarbons (PAHs). Fish
specimens were obtained 5 stations (Arvand, Jafari, Zangi, Samayeli, Bahrakan) along north
western coast of the Persian Gulf (Khuzestan Coast). PAHs concentration was measured by
HPLC method. Lysosomal membrane change was measured by NRR time method and
stability of red blood cell membrane was evaluated by EOF test. Total PAH concentrations in
the sediments and the liver tissue ranged between 113.50-3384.34 ng g-1dw, 3.99-46.64ng g-1
dw, respectively. Highest PAHs pollution was found at Jafari while the lowest was detected at
Bahrakan, with significant between these 2 stations. Values of mean RT of the dye ranged
from 34 (for the blood samples of mudskipper collected from Jafari site) to 78 minutes (for
the blood samples of mudskipper collected from Bahrakan). Preliminary results showed a
significant difference among stations except between Arvand and Zangi. Osmotic fragility
curves indicated that erythrocytes collected from mudskippers at Jafari were the most fragile
followed by Zangi> Arvand> Samayeli> and Bahrakan. The results suggest that lysosomal
membrane change and red blood cell membrane stability in B. dussumieri could be extended
as a biomarker of oil pollution in marine biomonitoring programs.

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Iranian Scientific Fisheries Journal
Volume 22, Issue 2 - Serial Number 83
November and December 2013
Pages 65-76

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