Karyology study of Spirlin (Alburnoides bipunctatus) in Zabol region

Authors

Abstract

The chromosomal spread and karyotype of Spirlin (Alburnoides bipunctatus) from Zabol
region were identified using tissue squashing techniques with injection of 0.5ml/100g body
weight of 0.01% Colchicines solution in fish fingerlings. Kidney and gill tissues were
extracted and chopped in KCl 0.075M for 30 min and fixed in Carnoy solution in 3 stages.
The chromosomal spreads were stained in 20% Gimsa for 20 min. From 34 chromosomal
spread counts, the results showed diploid chromosome number of this species 2n=50.
Karyotype composed of 9 metacentric, 14 submetacentric and 2 acrocentric or subtelocentric
chromosome pairs, and the number of chromosome arms (NF) was determined as NF=96

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