The effects of phytoestrogens Matricaria recutita on growth, maturation of oocytes in the three spot gourami (Trichogaster trichopterus)

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Abstract

Chamomile is a rich source of phytoestrogens these compounds have antioxidant, anticancer . In
this study, the effects of chamomile on growing oocytes in immature female gourami fish were
examined. Thus 60 immature female gourami fish with an average weight of 2-3 g of 6-treated
group were analyzed. Treatments in a completely randomized experimental design with a dose of
chamomile (10-20-30-50 mg/kg fish) along with control (no injection) and ethanol control (solvent
injection) and with three replicates were tested. The fish adaptation to the environment lasted 3
days. The extract injections were done for 20 days, in 10 periods, every other day. In order to
histological study ,three days after end of the experiment, the ovarian tissue was removed and
weighed, tissue processing and staining steps done with hematoxylin- eosin . At last the
histological structure of the ovaries and the average percentage of treated fish gonadal indices were
compared with the control group. According to our results of gonadal index, there was no
significant difference between the control groups with receiving Ethanol (p>0.05). Histological
results showed that increasing the dose of chamomile extract accelerate oocyte maturation in three
spot gourami . This is the fourth treatment (dose, mg / kg 50) to show its obvious. The difference
between oocyte diameter was observed with increasing doses of Chamomile and in comparison
with the control group, the difference was significant (p

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Iranian Scientific Fisheries Journal
Volume 23, Issue 1 - Serial Number 86
January and February 2015
Pages 85-94

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