This study was carried out to determine artificial breeding normatives of Russian Sturgeon (Acipenser gueldenstaedti). In this research, which was conducted in Shahid Marjani Breeding Center in late winter, 27 female and 13 male broodstocks were used. All broodstocks were provided from Turkeman and Mianghaleh fishing ground in the Caspian Sea. After maintaining them at Kurinski ponds and determination of germinal vesicle in the oocytes, 15 female and 13 male broodstocks were injected by 60-75 and 40-55 mg of pituitary hormone, respectively. After maturity duration, 10 female and 5 male broodstocks showed response to the hormone injection. Some parameters such as maturity duration, number of oocytes per gram, oocyte diameter, weight and bodymass of oocyte, motility and density of spermatozoid, duration of reaching to 4 and 16 divisions, incubation duration, fertilization percentage in each stage, mortality rate during incubation, number of larvae resulted from each broodstock, number of larvae per gram, weight of each larvae as well as some morphometric parameteres and age for each broodstock were determined.