Alarm cells and their distribution in different regions of Barbus sharpeyi's skin

Document Type : Research Paper

Authors

Ahvaz

Abstract

Skin of 20 normal, mature and same size fish (27.27±3.33cm) were subjected to microscopic assessment in different regions including lip, pectoral fin, head, back, side (on lateral line) and abdomen. The skin sections were prepared using routine paraffin embedding and stained by H&E and PAS. The alarm cells distribution were counted in 100ii length of epidermis. Microscopic results showed that voluminous cells existed in the middle to deeper layers of non-keratinization stratified squamous epidermis in all regions except for lip. These cells contained large and euchromatic nucleus in the center of cytoplasm. The cytoplasm has negative reaction to PAS staining. Histo-metrical results showed that shape and number of these cells vary in different regions of skin, with the differences being significant in all regions except for the back, abdomen and pectoral fin skin (P<0.05). Based on the distribution of alarm cells, we could divide the epidermis into four regions: 1- The skin of lip regio.....