EXTRACTION AND DETERMINATION OF PSP (SAXITOXIN AND DERIVATIVES) FROM SCALLOP

Document Type : Research Paper

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Abstract

Paralytic Shellfish Poison (Saxitoxin and derivatives) was extracted by HCL method from frozen scallops. The samples were collected from coastal areas of Iwate prefecture-Japan.
Toxicity of the samples was determined with mouse. Bioassay method Intra-peritoneal injection was done toddy strain 
19-21 grams male mouse, and death time was listed. In conclusion, 33.80, 38.40, 31.20 MU/g (or 777, 883, 718 STX/100gr) were obtained respectively as the toxicity of the samples of three different sampling areas. The toxicity for the areas was 9.7, 11 and 9 times more than standard level (80STX/100 g) and very dangerous for human consumption. The kind of toxin was determined by comparing HPLC graph of sample with standard saxitoxin HPLC graph.

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