Brachionus calyciflorus is a freshwater rotifer that is used as a live food for intensive production of freshwater fish larvae. In this research, the possibility of isolating, purifying and semi-intensive production of freshwater rotifer was investigated in laboratory condition. Sampling was done at Anzali Lagoon in autumn 2005 and the species was identified and isolated using invert microscope. For culture of the rotifer, EPA medium was used together with freshwater chlorella sp. at 1*106 cells/ml density. Rotifers were produced as batch culture. Primary density of rotifers in 11 balloons was considered to be 30 ind/mL Results showed that the maximum density of rotifer reached 453±43 ind/mlin the seventh day and mean growth rate per day was 0.50. The average of the lorica length and width was 204 and 167 microns and the average of the egg length and width was 121 and 95 microns, respectively. This experiment indicated that freshwater rotifer attains suitable growth parameters in laboratory condition in a relatively short time. Also, we successfully determined the protocol for semi-intensive artificial production of the species.
Ahmadifard, N., Abediyan, A., & Fallahi, M. (2007). ISOLATION AND SEMI-INTENSIVE CULTURE OF FRESHWATER ROTIFER BRACHIONUS CALYCIFLORUS OF ANZALI LAGOON. Iranian Scientific Fisheries Journal, 16(2), 1-10. doi: 10.22092/isfj.2007.114985
MLA
N. Ahmadifard; A. Abediyan; M. Fallahi. "ISOLATION AND SEMI-INTENSIVE CULTURE OF FRESHWATER ROTIFER BRACHIONUS CALYCIFLORUS OF ANZALI LAGOON". Iranian Scientific Fisheries Journal, 16, 2, 2007, 1-10. doi: 10.22092/isfj.2007.114985
HARVARD
Ahmadifard, N., Abediyan, A., Fallahi, M. (2007). 'ISOLATION AND SEMI-INTENSIVE CULTURE OF FRESHWATER ROTIFER BRACHIONUS CALYCIFLORUS OF ANZALI LAGOON', Iranian Scientific Fisheries Journal, 16(2), pp. 1-10. doi: 10.22092/isfj.2007.114985
VANCOUVER
Ahmadifard, N., Abediyan, A., Fallahi, M. ISOLATION AND SEMI-INTENSIVE CULTURE OF FRESHWATER ROTIFER BRACHIONUS CALYCIFLORUS OF ANZALI LAGOON. Iranian Scientific Fisheries Journal, 2007; 16(2): 1-10. doi: 10.22092/isfj.2007.114985
)