AN INVESTIGATION ON THE EFFECTS OF ANIONIC DETERGENT ON LIVER GLYCOGEN AND GLUCOSE IN COMMON CARP (CYPRINUS CARPIO)

Document Type : Research Paper

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Abstract

Water pollution due to chemical substances as anionic detergents causes various disorders in aquatic ecosystems. We studied the effects of detergent on common carp using 6 treatments containing 0.5, 1,2,4, 6 and 8mg/l and one control. We used aquariums at a capacity of 150 liters and in each aquarium 8 pieces of carp 10 to 12cm long weighing 35 to 40mg each was introduced. Physical factors were measured with digital multi-meter unit CS535. Temperature and hardness of water were 23-24oC and 196mg/l, respectively. Air pump was used during the experiment for providing oxygen. Sampling was carried out to assay liver glucose and blood glucose every 24 hour during 7 days. Liver glucose concentration was determined by glucose oxidize enzyme and spectro-photometric method at 546nm length. Liver glycogen was determined after separation and hydrolysis with the same method as for glucose. Results showed that blood glucose was increased as a result of exposure to detergent, but liver glycogen was decreased when detergent concentration was higher than 2mg/l. A reverse correlation coefficient between the two factors affirmed the findings (P<0.001, r=-0.96).

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Iranian Scientific Fisheries Journal
Volume 17, Issue 3
January and February 2008
Pages 161-164

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