EFFECTS OF CONCENTRATION AND DURATION OF ENRICHMENT OF ROTIFER BRACH IONUS PLICATILIS WITH COD OIL EMULSION WITH EMPHASIS ON FATTY ACIDS

Document Type : Research Paper

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Abstract

In this study, effects of concentration and length of enrichment of rotifer Brachionus plicatilis fatty acid composition and its population profile by cod liver-oil emulsion were tested. Rotifers pre-fed on yeast (Saccharomyces cerevisiae) were enriched with cod liver oil emulsion at three different concentrations (4, 8 and 12%). Rotifers were sampled after 0, 3, 6, 12 and 24 hours of enrichment. Considering total lipid present in rotifers as a function of the enrichment period, the increase was significant for 3 hours treatment while the same was not observed when raising the oil concentration (P<0.05). Increasing the enrichment period rather than the amount of oil present in the medium was found to be more efficient in increasing the n-3 highly unsaturated fatty acids (n-3 HUFA) level in rotifers. Rotifers showed a better incorporation of Eicosapentaenoic acid (EPA, 20:5n3) than Docosahexaenoic acid (DHA, 22:6n-3), regardless of the ratio between the two fatty acids in the emulsion.

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