Effect of dietary Lactobacillus casei and different levels of immunogen on the activities of immunological and hematological factors of Cyprinus carpio

The aim of this study was to evaluate the effects of synbiotic Lactobacillus casei PTCC 1608 and immunogen prebiotic on immunological and hematological factors of Cyprinus carpio. 300 fish (with the average weight of 40 g) were fed for 75 days with various diets including the diets containing 0.5 (A), 1 (B) and 1.5% (C) immunogen and L.casei probiotic with the concentration of 5×10⁷ CFU/g, treatment D contained only L.casei probiotic with the concentration of 5×10⁷ CFU/g and the control treatment without synbiotic (E). Results showed that the levels of white blood cells in the treatment A, B and C on day 60 were significantly different as compared to day 0 and the control group (P<0.05). The levels of red blood cells in the treatment A, B, C and D on day 30 were at the highest level and were significantly different as compared to day 0 and the control group (P<0.05). The levels of lysozyme in the treatment C and D until day 30 and in other treatments until day 60 were significantly different from the control group (P<0.05). The administration of synbiotic in the treatment C and D on respiratory burst activities on day 30 and in other treatments until day 60 and the level of complement on day 60 in the treatment A were significantly different as compared to those on day 0 and the control group (P<0.05). The results suggested that dietary administration of synbiotic can improve some immunological and hematological parameters and the best level of immunogen was 1% with synbiotic.