a

Ellis, A.E:,1990. Lysozyme Assays:In Stolen, J.S., Fletcher, T.C., Anderson, D.P., Roberson, B.S. andVan Muiswinkel ,W.B., (ed)Techniques in :Fish Immunology,edn. Fair Haven,: SOS Publications . London, England ,pp. 101-103.
Faghani, T., Soltani, M., Shamsae, M.and Matinfar, A., 2013. The effect of dietary natural Astaxanthin(Haematococcus pluvialis) on the growth parameters, carcases and liver chemical composition injuvenile beluga (Huso huso Linnaeus, 1758). Journal of Marine Biology, 5: 69-78.
Firouzbakhsh, F.; Noori, F.; Khalesi, M.K. and JaniKhalili, K., 2011. Effects of a probiotic, protexin, on the growth performance and hematological parameters in the Oscar (Astronotus ocellatus) fingerlings. Fish. Physiology andBiochemistry, 29: 222-229. DOI: 10.1007/s10695-011-9481-4.
Ghosh, S.; Sinha, A. and Sahu, C.,2008. Dietary probioticsupplementation in growth and health of live-bearingornamental fishes. Aquaculture Nutrition, 52: 227-277. DOI: 10.1111/j.1365-2095.2007.00529.x
 
Gibson, G.R. and Roberfroid, M.B., 1995. Dietarymodulation of the human colonic microbiota: introducingthe concept of prebiotics. J. Nutr. 521:5205-5252. DOI: 10.1093/jn/125.6.1401.
 
Harikrishnan, R., Kim, J., Balasundaram, C. and Heo, M., 2012.Immunomodulatory effects of chitin and chitosan enriched diets in Epinephelus bruneus against Vibrio alginolyticus infection. Aquaculture, 326: 46-52.  DOI: 10.1016/j.aquaculture.2011.11.034.
Hoseinifar, S.H., Zoheiri, F., Dadar,M., Rufchaei,R. and Ringø, E., 2016. Dietary galactooligosaccharide elicits positive effects on non-specific immune parameters and growth performance in Caspian white fish (Rutilus frisii kutum) fry. Fish and Shellfish immunology, 56:467- 472. DOI: 10.1016/j.fsi.2016.08.001.
Hoseinifar, S.H.; Khalili, M.; Khoshbavar Rostami, H. and Esteban,  M.A., 2013. Dietary galactooligosaccharide affects intestinal microbiota, stress  resistance,  and performance of Caspian roach (Rutilus rutilus) fry. Fish andShellfish Immunology, 35:1416-1420. DOI: 10.1016/j.fsi.2013.08.007.
Hoseinifar, S.H., Mirvaghefi, A., Merrifield, D.L., Amiri, B., Yelghi, S. and Bastami, K., 2011.The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose. Fish Physiology and Biochemistry,37:91-96. DOI: 10.1007/s10695-010-9420-9.
Hoseinifar, S.H., Zare, P. and Merrifield, D.L.,2010. The effects of inulin on growth factors and survival of the Indian white shrimp larvae and postlarvae (Fenneropenaeus indicus). Aquaculture Research, 41:348-352.  DOI: 10.1111/j.1365-2109.2010.02485.x.
 
Hoseinifar, S.H.,Sharifian,M., Vesaghi,M.J., Khalili,M. and Esteban,M.A., 2014. The effectsof dietary xylooligosaccharide on mucosal parameters, intestinal microbiotaand morphology and growth performance of Caspian whitefish (Rutilus frisiikutum) fry, Fish and Shellfish Immunology, 13: 231-236.  DOI: 10.1016/j.fsi.2014.05.009.
Ibrahem, M.D., Fathi, M., Mesalhy, S.and  Abd, E.A.,2010.Effect of dietary supplementation of inulin and vitamin C on the growth, hematology, innate immunity, and resistance of Nile tilapia (Oreochromis niloticus). Fish and Shellfish Immunology,29:241-246. DOI:10.1016/j.fsi.2010.03.004.
Jenabi Haghparast, R., Meshkini, S. and Tokmehchi, A., 2013. Effects prebiotic Mannanoliosaccharide on growth and immune in Oncorhynchus mykiss. Journal of Veterinary Researches, 68: 375-382.
Kahkesh, M. and Roomiani, L., 2018. Effect of dietary Lactobacillus casei and different levels of immunogen on the activities of immunological and hematological factors of Cyprinus carpio. ranian Scientific Fisheries Journal, 26; 140-151. DOI: 10.22092/ISFJ.2018.115841
 
Khodadadi, A., Haghighi, A., Malekinejad, H., Tukmechi, A. and Afsharnasab M., 2018. Dietary effect of celmanax® on growth factor, intestine histology and yersiniosis  resistance in rainbow trout (Oncorhynchus mykiss). Iranian Scientific Fisheries Journal, 26;126-139. DOI: 10.22092/ISFJ.2018.115694.
Kumar, S., Sahu, N.P., Pal, A.K., Choudhury, D., Yengkokpam, S. and Mukherjee, S.C.,2005. Effect of dietary carbohydrate on hematology, respiratory burst activity and histological changes in Labeo rohita juveniles. Fish. Shellfish Immun. 19: 331-344. DOI:org/10.1016/j.fsi.2005.03.001.
Liu, F., Shi, H., Guo, Q., Yu, Y., Wang, A., Lv, F. and Shen, W.,2016. Effects of astaxanthin and emodin on the growth, stress resistance and disease resistance of yellow catfish (Pelteobagrus fulvidraco). Fish and Shellfish Immunology, 51: 125-135.DOI: 10.1016/j.fsi.2016.02.020.
Mokhtari, M., Imanpour, M.R., Hajimoradloo, A.M. and Hosseinifar, H.S.,2016.Effects of probiotic Bactocel and Prebiotic galactooligosaccharide on growth, survival, blood parameters and resistance against salt stress in Trichogaster trichopterus. Journal ofAnimalEnvironment, 3:199-206.
Ravelo, C.; Magariños, B.; Herrero, M. C.; Costa, L.; Toranzo, A. E. and Romald, J. L., 2006. Use of adjuvanted vaccines to lengthen the protection agaist Lactococcusis in rainbow trout (Oncorhynchus mykiss).Aquaculture, 251: 153-158. DOI: 10.1016/j.aquaculture.2005.05.027.
Soleimani, N., Hosseinifar, S.H., Merrifield, D.L., Barati, M.  andHassan Abedi, Z.,2012. Dietary supplementation of fructoologosaccharide (FOS) improves the innate immune response, stress resistance,digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish and Shellfish immunology, 32: 316-321. DOI: 10.1016/j.fsi.2011.11.023.
Sugita, H.; Okano, R.; Suzuki, Y.; Iwai, D.; Mizukami, M.; Akiyama, N. and Matsuura, S.,2002. Antibacterial abilities of intestinal bacteria from larvae and juvenile Japanese flounder against fish pathogens.FisheriesScience,2: 1004-1011.
Wahli, T., Verlhac, V., Griling, P., Gabaudan, J. and Aebischer, C.,2003. Influence of dietary vitamin C on the wound healing process in rainbow trout (Oncorhyncus mykiss) Aquaculture, 225: 371-386.  DOI: 10.1016/S0044-8486(03)00302-8.
Zhou, Q-C.; Buentello, J.A. and Gatlin Iii,D.M., 2010. Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum (Sciaenops ocellatus). Aquaculture, 207: 212-219.  DOI: 10.1016/j.aquaculture.2010.09.003.
 
 
 
 
 
ct of  galactooligosaccharide prebiotic on growth performance, survival  and several of innate immunity parameters of rainbow trout (Oncorhynchus mykiss) larvae
Ali M¹.,Akbary P^2*., Soltanian S¹. and Gholamhosseini A^1*[1]paria.akbary@gmail.com
1. Aquatic Animal Health and Diseases group, Faculty of Veterinary Medicine, Shiraz University
2- Fisheries group, Faculty of Marine Sciences, Chabahar Maritime University
Abstract
 
Prebiotics are non-digestive food ingredients which beneficially affect the host by selectively stimulating the growth and activity of health-promoting bacteria in the intestinal tract. This experiment was conducted to evaluate the effect of dietary supplementation of galactooilgosaccharide on the growth performances, survival and several of innate immunity parameters of rainbow trout (Oncorhynchus mykiss) larvae for 60 days.In this experiment, 360 of larvae (with average weight of 0.13±0.01g) in 4 treatments and 3 replicates (n=30 in each replicate) in a completely randomized design was divided and fed with diets containing 0, 1, 2 and 3 percent galactooligosaccharide respectively.The present results showed that highest WG, SGR and PER were observed in the diet containing 2 and 3 percent of dietary galactoologosaccharide which had a significant difference compared with other treatments (p<0.05). The highest, survival, total protein, albumin and globulin levels were observed in treatments containing 2 percent of dietary galactooligosaccharide which showed a significant difference compared with other treatments (p<0.05). Finally, the present results suggest that diets containing 2 percent of dietary galactooligosaccharide could improve growth, survival and innate immunity responses of rainbow trout
 



[1] *Corresponding Author