Isolation of Aeromonas hydrophila effective phage from wastewater and using it in order to control the bacterial growth in minced meat of common carp (Cyprinus carpio)

Document Type : Research Paper

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Abstract

In this study is to isolate and identify the molecular identity of effective phage on Aeromonas hydrophila in Urmia Urban Sewage System and control the experimental contamination in common minced carp fillet. For to isolate bacteriophage in the treated water, the existing bacteria were destroyed by pure chloroform in order to release the existing phage. Also the standard strains of Aeromonas hydrophila were used to evaluate the antibacterial assay of isolated bacteriophage (via CODE PTCC1310). Likewise, fish samples were prepared as fresh and minced. Then minced meat was contaminated with 108 CFU/g of Aeromonas hydrophila and four levels of effective bacteriophage, 108, 107, 106 and 105 PFU/g respectively, per one gram of meat were added and mixed. Meat samples containing negative and positive (2ppm of nisin) control and four levels of bacteriophage (108, 107, 106 and 105 PFU/g) were stored in refrigerator for a week. During the storage, sampling was done for bacterial count and bacteriophage titration. Results showed that effective phage on Aeromonas hydrophila isolated from Urmia sewage system could prevent the growth of bacteria. Also, findings showed that the levels of 10000 and 100000 PFU/g  could significantly prevent the growth of Aeromonas hydrophila in common minced carp meat. It can be concluded that the phage effective on Aeromonas hydrophila in Urmia sewage system can suitably be used for biological control in minced meat of common carp (Cyprinus carpio).

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Iranian Scientific Fisheries Journal
Volume 30, Issue 4
November and December 2021
Pages 13-25

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