Isolation, replication and transmission of VP28 gene of shrimp white spot virus into Chlorella vulgaris microalgae using electroporation

Document Type : Research Paper

Authors

10.22092/isfj.2022.127536

Abstract

White spot disease (WSD) is one of the most important diseases in shrimp farming, which has caused a decrease in production and severe economic damage all over the world, including Iran. Mortality caused by this severe disease is very high and reaches 100% within 3 to 10 days from the onset of the disease. According to global statistics, about 300,000 tons of shrimp are lost due to this disease every year in the world. For example, within the years 2004, 2006, 2007, 2015, 2016 and 2019, the outbreak of this disease caused millions of dollar losses to the producers in Iran. Since the VP28 viral protein gene is one of the most important white spot disease virus envelope proteins and plays a role in sticking to and penetrating shrimp cells, and since microalgae is the most important source of food for shrimp in the larval stage, So it can be a suitable host for making  a vaccine. So in this study, samples of shrimp (Penaeus indicus) suspected of white spot disease from Abadan (Khuzestan province) breeding ponds were transferred to the laboratory of Razi Vaccine and Serum Institute of Arak branch and the genomic DNA of the virus was extracted using proteinase K and phenol/chloroform methods. The VP28 gene was proliferated by PCR method, inserted into pCAMBIA1304 vector and finally transferred to Chlorella vulgaris microalgae using electroporation method.

Keywords

Iranian Scientific Fisheries Journal
Volume 31, Issue 3
September and October 2022
Pages 59-69

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