Evaluation of some biofilter substrates in freshwater recirculation system

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Abstract

The nitrifying bacteria acting on various biofilter substrates including: scoria, P.V.C., marble and
zeolite were evaluated. The experiment started with manufacture of a recirculation system with 130
liter fresh water volume in 7 l.min-1 flow rate in 3 replicate for every treatment. The volume of each
biofilter was 60×40×30cm and after achievement to stable environmental conditions the nitrifying
bacteria at a concentration of 105ml-1were inoculated in each biofilters. Daily 3-10mg.l-1 of NH4Cl
was added to biofilters. The procedure was conducted by exposure of Ammonium and Nitrate
concentrations and pH in biofilters. The results showed that the Scoria can be reliable substrate as
biofilter, in which provide the best substrate for the growth and attachment of the nitrifying
bacteria which can reduce the retention time of a biofilter from 40-60 to 15 days.

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Iranian Scientific Fisheries Journal
Volume 21, Issue 3 - Serial Number 80
November and December 2012
Pages 129-136

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