بررسی مقایسه‎ای نگهداری برخی جلبک‏های آب شیرین به روش‎های انجماد خشک و محافظت سرمایی

نوع مقاله : مقاله پژوهشی

نویسندگان

چکیده

این تحقیق به منظور بررسی امکان نگهداری جلبک‏های آب شیرین به روش انجماد خشک و محافظت سرمایی و مقایسه قابلیت رشد آنها انجام شد. 3 گونه جلبک آزمایشی شامل Chlamydomonas moewusii، Scenedesmus obliquusو Chlorococcum olefaciens پس از تهیه محیط کشت زایندر در 6 ارلن به مدت 20 روز کشت داده شدند. سپس هر گونه با نه تکرار (سه تکرار برای هر یک از روزهای اول، پنجم و دهم) به روش‏های انجماد خشک و محافظت سرمایی برای نگهداری آماده گردید. جلبک‏ها در ادامه، کشت مجدد شده و رشد آنها با سنجش کلروفیل a طی روزهای اول، پنجم و دهم پس از خروج از شرایط حفاظتی بررسی گردید. تعداد 9 ارلن نیز برای هر گونه جلبکی تنها با افزودن محیط کشت اختصاصی جلبک به عنوان تیمار شاهد آماده گردید تا مقیاس مشابهی برای بررسی رشد جلبک‏ها در روزهای یکسان فراهم آید. نتایج نشان داد که بیشترین رشد هر سه گونه در انجماد خشک پس از گذشت ده روز از کشت مجدد به دست آمد که به شکل معنی‏داری (p<0.05) بالاتر از تیمار شاهد بود. با این وجود تفاوت معنی‏داری در میزان رشد ScenedesmusobliquusوChlorococcumolefaciensپس از محافظت سرمایی و انجماد خشک به دست نیامد (p>0.05). یافته‏های پژوهش حاضر نشان داد که هر دو روش حفاظتی برای نگهداری سه گونه جلبک سبز مطالعاتی مناسب است، گرچه روش انجماد خشک ‏در گونه Chlamydomonasmoewusii نتایج بهتری را ارایه نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of some freshwater green algae conservation using the of freeze-drying and cryopreservation methods

نویسندگان [English]

  • E Fayazi Atdotan
  • H Rajabi Islami
چکیده [English]

This study was conducted to evaluate the possible conservation of freshwater green algae using the freeze-drying and cryopreservation methods and comparative of their growth potential. Three experimental algae including Chlamydomonas moewusii, Scenedesmus obliquus, and Chlorococcum olefaciens were cultured for 20 days in 6 Erlenmeyer flasks after preparation of Zinder growth medium. Each algal strain was preserved according to freeze-drying and cryopreservation protocols with 9 replications (3 replications for the days of 1, 5, and 10). Algae were then re-incubated and their growth was evaluated in 1, 5, and 10 days after preservation. Another 9 Erlenmeyer flasks for each algal species were prepared as control treatment by adding the respective growth medium to consider the growth of algae in the same days with similar scale. Highest growth of all experimental algae was obtained after 10 days which was significantly higher than the corresponding control treatments (p<0.05). However, no significant difference was observed in the growth of Scenedesmus obliquus andand Chlorococcum olefaciens after freeze-drying and cryopreservation (p>0.05). Results of the present study illustrated that both preservative methods are suitable for maintenance of experimental algae, although freeze-drying provided better results in Chlamydomonasmoewusii.

کلیدواژه‌ها [English]

  • Chlamydomonas moewusii
  • Freeze- drying
  • SCENEDESMUS OBLIQUUS
  • Chlorococcum olefaciens
  • Freeze- drying
  • Cryopreservation

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مجله علمی شیلات ایران
دوره 24، شماره 2 - شماره پیاپی 2
آذر و دی 1394
صفحه 103-114

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