نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The toxic effects of water soluble fraction (WSF) of crude oil on the gills of juvenile
kutum roach, Rutilus frisii kutum, were investigated. The juvenile (1-2g) kutum roach were
collected in summer 2008 from the Kolmeh Hatchery Center, Golestan province, and
acclimatized to laboratory conditions through keeping them in a 300 liter tank with dechlorinated
water for a minimum of 8 days. Six different types of WSF concentrations were
made to obtain LC5096h including 27, 29.25, 31.5, 33.75, 36 and 38.25ppm. Then, 10
fingerlings of R. frisii kutum were exposed to the WSF concentrations, for 96 hours.
Considering mortality rate of the juveniles in 96 hours and using Probit value statistical
analysis, LC5096h was obtained at 33.95ppm. Two different concentrations (0.1 LC50 and
LC50) of WSF were prepared for further assessments and after 24 and 96h of exposure, 3
individual fish were collected from each aquarium for histopathological studies. The gill
sections with 3 micron thickness were prepared, stained using H & E, and examined by light
microscope. General damages after observation included: Epithelial lifting, congestion in
filament and lamellae, clubbing, fusion, hypertrophy of epithelial cells of lamellae, different
levels of hyperplasia between lamella and aneurysms. Pathological studies showed that the
WSF of crude oil causes serious damage in gills of juvenile R. frisii kutum, leads to
malfunction of this organ which will harm homeostasis of the fish.
Corresponding author
کلیدواژهها [English]
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