نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Rainbow trout, Oncorhynchus mykiss is the most important cold water farmed fish in
Iran. Several cultivated stocks have been imported to Iran and some fish farms have focuesd
on the culture and breeding of these stocks all around the country. In this study, the genetic
structure of Spanish and American stocks of rainbow trout was investigated using 4 pairs of
microsatellite markers and 30 specimens of fish from each stock. Allele sizes at OMY77,
OMY325, OMM1329 and OMM1332 loci were in the range of 102-178, 100-150, 122-198,
172-204 bps respectively. Average number of observed alleles in American and Spanish
stocks was 11 and 11.5, respectively. Average number of effective alleles in Spanish and
American stock was 8.65 and 8.03, respectively. The mean of observed heterozygosity for
American and Spanish stocks were calculated as 0.59 and 0.63, respectively. The mean of
expected heterozygosity for both stocks was very similar (around 0.86). The results showed a
significant deviation from Hardy-Weinberg equilibrium at seven out of eight loci à stock.
AMOVA test showed low (7%) genetic diversity between stocks, while the most (93%)
genetic diversity was observed within stocks. Genetic identity and genetic distance between
stocks were 0.831 and 0.186, respectively. Fixation index FST was calculated based on allelic
frequency between two stocks was 0.012 with no significant difference between 2 stocks. The
results of this study showed that while there was considerable genetic diversity within
Spanish and American stocks, two stocks were very similar and showed very insignificant
genetic differentiation based on 4 microsatellite studied loci.
کلیدواژهها [English]
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