تاثیر جایگزینی غذای تجاری با کرم خاکی بر شاخص‌های رشد، بقاء، تعداد لاروهای حاصل و مقاومت لاروها در برابر استرس شوری در ماهی دم شمشیری (Xiphophorus helleri)

نویسندگان

1 دانشگاه ارومیه

2 دانشگاه ارومیه- دانشکده منابع طبیعی - گروه شیلات

چکیده

در مطالعه حاضر تاثیر جایگزینی کرم­خاکی Eisenia foetida با غذای تجاری بر رشد، بقاء، تعداد لاروهای حاصل و مقاومت لاروها در برابر استرس­های محیطی ماهی دم شمشیری (Xiphophorus helleri) مورد بررسی قرار گرفت. آزمایش به صورت طرح کاملاٌ تصادفی در 4 تیمار شامل، تیمار 1 (100% غذای تجاری)، تیمار 2 (25%کرم­خاکی+75%غذای تجاری)، تیمار 3 (50%کرم­خاکی+50%غذای تجاری) و تیمار 4 (75%کرم­خاکی+25% غذای تجاری) با سه تکرار طراحی شد. ماهیان دم­شمشیری به میزان 3 درصد وزن بدن تغذیه شدند. بعد از یک دوره آزمایش 60 روزه، شاخص­های رشد و بقاء ماهی دم شمشیری تغذیه شده با جیره­های مختلف غذایی تحت تاثیر نوع جیره قرار نگرفت(05/0<p) اما بطور معنی­داری تحت تاثیر جنسیت قرار گرفت (05/0>p). درصد افزایش وزن،  نرخ رشد ویژه و بقاء بطور معنی­داری در جنس ماده بیشتر از جنس نر بود. بیشترین تعداد لارو حاصل در تیمار 2 (25%کرم­خاکی) مشاهده شد که نسبت به تیمار شاهد اختلاف معنی­داری داشت (05/0>p). در تیمار 4 (75%کرم­خاکی) لاروها در برابر استرس محیطی بطور معنی داری مقاومت کمتری نسبت به سایر تیمارها نشان دادند (05/0>p). با توجه به تحقیق حاضر تعداد لاروهای حاصله در ماهیان دم شمشیری تغدیه شده با جایگزینی 25% افزایش معنی دار و چشمگیری نسبت به تیمار شاهد داشت. از اینرو استفاده از سطوح پایین جایگزینی 25 درصد پودر کرم خاکی به جای غذای تجاری در پرورش ماهیان دم شمشیری می­تواند برای تغذیه این ماهیان مناسب باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of commercial food replacement with earthworm (Eisenia foetida) on growth, survival, the number of larvae and their resistance to salinity stress in swordtail fish (Xiphophorus helleri)

نویسندگان [English]

  • N. Ahmadifard 1
  • M. Sotudeh 2
  • A. Imani 1
چکیده [English]

In this study, the effect of commercial food replacement with earthworm (Eisenia foetida) on growth, survival, the number of larvae and their resistance to larvael salinity stress in Swordtail fish (Xiphophorus helleri) was investigated. A completely randomized design experiment comprising of four treatments: T1 (100% commercial food), T2 (25% Earth worm +75% Commercial food), T3 (50% Earth worm + 50% commercial food), T4 (75% Earth worm + 25% Commercial food) were performed in triplicate. Swordtail fish were fed with 3% biomass and 3 times per day. After a 60-day experimental feeding period, growth factors and survival rate of fish fed with different diet treatments were not affected. However, the growth factors and survival were affected by sex and the weight gain, specific growth rate and survival rate of females was significantly higher than males. The highest number of born larvae was observed in T2 which was significantly more than control. (p<0.05). In T4, larvae showed the lowest resistance to salinity stress test (p<0.05). Results showed that number of born larvae significantly increased in 25% replacement than control, so the Swordtail fish could be fed with low levels (25%) of earthworm without any unfavorable effects on the growth and survival rate and their larval resistance to salinity stress test. 

کلیدواژه‌ها [English]

  • Earth worm
  • Swordtail Fish
  • Salinity stress test
  • born larvae
Apolinario, M.O., Correia, E.S. and A.S. Souto., 1998. Utilization of earthen worm (Eisenia foetida) in the feeding of Penaeus vannamei post larvae. Aquaculture-Brazil, sustainable developement 26 November, 1998, Proceedings, Volume 2: Scientific papers. Recife, Brazi. Axelrod, A. H. and Wischnath, L., 1991. Swordtails and platies. T. H. F. Publication, Ink., Neptune City, NJ , USA. BOANR, 1993. Board of Agriculture and Natural Academics Press. Washington DC. 68P. Correia, E.S., Pereira, J.A., Apolinario, M.O., Horowitz, A. and Horowitz, S., 2002. Effect of pond aging on natural food availability and growth of the freshwater prawn Macrobrachium rosenbergii. Aquacultural engineering, 26(1), pp. 61-69 Dedeke, G., Owa, S., Olurin, K., Akinfe, A. and Awotedu, O., 2013. Partial replacement of fish meal by earthworm meal (Libyodrilus violaceus) in diets for African catfish, Clarias gariepinus. International Journal of Fisheries and Aquaculture. 5: 229-233. Degani, G. and Boker, R., 1992. Sensitivity to maturation inducing steroids and gonadotropin in the oocytes of blue gourami Trichogaster trichopterus (Anabantidae, Pallas, 1770). Gen. Comp. Endocrinol., 85: 430-439. Edwards, R.W. and Densen, J.W., 1980. Fish from sewage. Applied Biology. 5 (1): 70-211. Fadaee, R., 2012. A review on earthworm Esienia fetida and its applications. Annals of Biological Research. 3 (5): 2500-2506. FAO, 2004. The state of world Fisheries and aquaculture, Rom, Italy. Girri, S.S., Shoo, S.K., Sahu, B.B., Sahu, A.K., Mohanty, S.N., Mohanty, P.K. and Ayyappan, S., 2002. Larval survival and growth in walago att (Bloch and Schneider): Effects of light, photoperiod and feeding regimes. Aquaculture. 213: 157-161. Azimirad, M., Meshkini, S., Ahmadifard, N. and Hosainifar, S.H., 2016. The effects of feeding with synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (Pterophyllum scalare). Fish and Shellfish Immunology, 44: 516–522 Kim, J., Masses, K.C. and Hardy, R.W., 1996. Adult Artemia as food for first feeding coho salmon (Oncorhynchus kitsch). Aquaculture. 144: 217-226. Kostecka, J. and Paczka, G., 2006. Possible use of earthworm Eisenia fetida biomass for breeding Aquarium fish. European journal of soil Biology. 42: 231-233. Lim, L.C., Cho, Y.L., Dhert, P., Wong, C.C., Nelis, H. and Sorgeloos, P., 2002. Use of decapsulated Artemia cysts in ornamental fish culture. Aquaculture Research. 33: 575–589. Mousavi-sabet, H. and Ghasemnezhad, H., 2013. Masculinization, mortality and growth rates of Swordtail Xiphophorus hellerii (Poeciliidae) affected by methyltestosterone. Poeciliid Research. 3(1): 7-11. Nandeesha, M., Srikanth, G., Basavaraja, N., Keshavanath, P., Varghese, T., Bano, K., Ray, A. and Kale, R.D., 1988. Influence of earthworm meal on the growth and flesh quality of common carp. Biological Wastes. 26: 189-198. Ng, W-k., Liew, F-l., Ang, L-P. and Wong, K-W., 2001. Potential of mealworm (Tenebrio molitor) as an alternative protein source in practical diets for African catfish, Clarias gariepinus. Aquaculture Research. 32: 273–280 Óscar Pereira, J. and Gomes, E.F., 1995. Growth of rainbow trout fed a diet supplemented with earthworms, after chemical treatment. Aquaculture International. 3: 36-42. Patino, R. and Thomas P., 1990. Effect of gonadotropin on ovarian intrafollicular processes during the development of oocytes maturational competence in teleost, the Atlantic coraker: evidence for two distinct stages of gonadotropic control of final oocyte maturation. Biol. Reproduction, 43: 818-827. Rezvani, A., MojaziAmiri, B., Manouchehri, H. and Abadian, R., 2011.Measurement of Gonaddal DevelOpment of Astronotus ocellatus (Cuvier, 1829) as a Result of Feeding Earthworm (Eisenia foetida). International Journal of research in Fisheries and Aquaculture. 1(1):11-13. Sabine, J. 1978. The nutritive value of earthworm meal. Cited by: Edwards, C. A. and A. Niederer. 1988. The production and processing of earthworm protein. pp. 169-180. Sigh, B.N., Sinha, V.R. and Chakraborty, D.P., 1983. Effect of protein quality and temperature on the growth of fingerlings of Rohulabeo rohita, Paper Presented at the World Symposium of Fish Nutrition and Fish Feed Technology. Hambour Germany. Stafford, E. A. and Tacon. A.G.J., 1984. Nutritive Value of the Earthworm, Dendrodrilus subrubicundus, Growth on Domestic Sewage, in Trout Diets. Agricultural Wastes 9: 249-266. Stafford, E.A. and Tacon, A.G.J., 1985. The nutritional evaluation of dried earthworm meal (Eisenia foetida, Savigny, 1826) included at low levels in production diets for rainbow trout, Salmo gairdneri Richardson. Aquaculture Research. 16: 213-222. Stafford, E. A. and Tacon. A.G. J., 1988. The use of earthworm as a food for rainbow trout Salmo gairdneri. In: Earthworms in waste and environmental management. C. A. Edwarrds and E. F. Neuhauser (eds). Academic Publishing, The Hague, the Netherlands, pp 181- 192. Stickney, R., 2000. Encyclopedia of aquaculture, John Wiley and Sons, Inc, Printed in the United State of America, 1063P. Storebakken, T., Shearer, K.D., Baeverfjord, G., Nilsen, B.G., Asgard, T. and Scotttde Laporte, A., 2000. Digestibility of macronutrients, energy, and amino acids, absorption of elements and absence of intestinal enteritis in Atlantic salmon, Salmo salar fed diets with wheat gluten. Aquaculture 184:115-132. Suzuki, K., Nagahama, Y. and Kawauchi, H., 1988. Isolation and characterization of two distinct gonado tropins from chum salmon pituitary gland. Gen. Comp. Endocrinol. 71 (1): 293-301. Tacon, A.G.J., Stafford, E.A. and Edwards, C.A., 1983. A preliminary investigation of the nutritive value of three terrestrial lumbricid worms for rainbow trout. Aquaculture 35:187-199. Townshend, T.J. and Wooton, R.J., 1984. Effects of food supply on the reproduction of the convict cichilid, Cichlasoma nigrofasciatum. J. Fish Biolo, 24: 91-104. Tuan, N.N., Pucher, J., Becker, K. and Focken, U., 2015. Earthworm powder as an alternative protein source in diets for common carp (Cyprinus carpio L.). Aquaculture Research doi:10.1111/are.12743. Tukmechi, A., Rahmati Andani, H. R., Manaffar, R. and Shikhzadeh, N., 2011. Dietary administration of beta-mercapto-ethanol treated Sacchromyces cerevisiae enhanced the growth, innate immune response and disease resistance of the rainbow trout, Oncorhynchus mykiss. Fish and Shellfish Immunology 30:923-928. Velasquez, L., Ibanez, I., Herrera, C. and Oyarzun, M., 1991. A note on the nutritional evaluation of worm meal (Eisenia feotida) in diets for rainbow trout. Animal Production. 53: 119-122. Watanabe, T., Verakunpiriya, V., Watanabe, K., Kiron, V. and Satoh, S., 1997. Feeding of Rainbow Trout with Non-Fish Meal Diets. Fisheries Seience. 63(2), 258-266.