نوع مقاله : مقاله پژوهشی
نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Pheromones are molecular signals of hormones or their derivatives that affect the same species in the environment outside the body. The effect of ovary extract of psyllium as a pheromone-containing substance (PGF2α) on pheromone induction and the process of final sexual maturation and changes in male sperm indices of this fish have been investigated and compared in different treatments. Male fish with an average weight of 45 ± 200 g were released in 300 liter fiberglass ponds and after adapting the fish to the environment (for 48 hours), the experiments were performed in 5 experimental groups (one control group, one prostate gland control group and 3 groups Treatment) in which each group consisted of 9 adult male fish. Different concentrations of ovarian extract were added to the water in three treatments of 5, 10 and 15 mg / l as a bath. After 24 hours, the fish were anesthetized and fish biometrics was performed by measuring length and weight. After blood sampling, fish gonads were weighed. The results showed that pheromone induction of ovarian extracts and their positive effect on increasing the rate of sperm counts. Gonadosomatic index as well as testosterone levels at 10 and 15 mg / l treatment and prostaglandin treatment (PGF2α) were significantly increased compared to the control (p <0.001). Sperm density and spermatocrit in the treatment of 15 mg / l (T3) had a significant decrease compared to the control, but the indices of sperm motility rate and sperm motility percentage compared to the control did not show a significant difference. Relative sperm volume in all treatments was affected by pheromone extract and a significant increase was observed compared to the control in T3 treatment (p <0.001). Administration of pheromone extract resulted in control of male reproductive fish reproduction in all experimental groups.
کلیدواژهها [English]
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