نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The immunolocalization of Na+,K+-ATPase rich-cells (chloride cells) and their distribution
pattern in smolt Salmo trutta caspius fries of the Caspian Sea weighing 5, 15, 25 grams during
freshwater adaptation was studied in 2008. Gill samples were fixed in Bouin s solution, and
after hydration, the samples were paraffinaized and sectioned. Na+,K+-ATPase localization
was performed using IgG 5 antibody and immunohystoshimy technique. In order to count
cells in gill area, immunofluorescence light microscopy pictures was analyzed using Image
Tool 2.1 software. Chloride cells were found on gill arch, lamellae and filament. Filamentary
chloride cells and their total number (chloride cells in lamellae and filament) had no
significant difference in all 5, 15, 25g specimens but lamellar chloride cells in 5g fries was
significantly decreased. Also, percentage of lamellae chloride cells in 15g specimens and
those of filament chloride cells in 5g fries was higher than other weights. According to our
results, weight has important impact on osmoregulation ability in same age fishes. Fries with
higher weight can resist salinity stress after migration to Caspian Sea through production of
more chloride cells and change in their position but those which remain in freshwater for a
long time, would adapt easily to the new environment.
*Corresponding author
کلیدواژهها [English]
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